ProteoMiner™ Sequential Elution Reagents #1633003

ProteoMiner™ Sequential Elution Reagents

10 preps, protein preparation reagents for eluting proteins into multiple fractions, columns not included, for use with #1633006 and #1633007

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Number Description Options
163-3003-MSDS ProteoMiner Sequential Elution Kit English

Use the ProteoMiner Sequential Elution Reagents with the ProteoMiner Protein Enrichment Small-Capacity or ProteoMiner Protein Enrichment Large-Capacity Kits for successive elution of populations of proteins with different characteristics during the enrichment of proteins in your sample.

ProteoMiner Protein Enrichment Kits are designed to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins in 10 or 50 mg of sample. The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away. After washing, the ProteoMiner Sequential Elution Reagents can then be used to fractionate the retained proteins.

  • Four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics
  • Compatible with a wide range of input sample types
  • Can be used for differential expression analysis
  • Sequential elution has been optimized for samples for SELDI; eluted fractions are not compatible with direct use of fractions in 2-D gel electrophoresis

Kit Contents

  • Elution Reagent 1, 1 M sodium chloride, 20 mM HEPES, pH 7.4, 5 ml
  • Elution Reagent 2, 200 mM glycine, pH 2.4, 5 ml
  • Elution Reagent 3, 60% ethylene glycol, 5 ml
  • Elution Reagent 4, 33.3% 2-propanol, 16.7% acetonitrile, 0.1% trifluoroacetic acid, 5 ml
  • Plasma Preparation Buffer, 1 M sodium citrate, 20 mM HEPES, pH 7.4, 1 ml
  • Capped 2 ml centrifuge tubes, 30

Product Options

ProteoMiner Protein Enrichment and Elution Kits Capacity Number of Samples Catalog Number
Small-Capacity Enrichment Kit ≥10 mg 10 1633006
Introductory Small-Capacity Enrichment Kit ≥10 mg 2 1633008
Large-Capacity Enrichment Kit ≥50 mg 10 1633007
Introductory Large-Capacity Enrichment Kit ≥50 mg 2 1633009
Sequential Elution Reagents   10 1633003
Small-Capacity Elution Kit (combines 1633006 and 2633003) ≥10 mg 10 1633010
Large-Capacity Elution Kit (combines 1633007 and 2633003) ≥50 mg 10 1633011

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Supporting Documentation

  • ProteoMiner Protein Enrichment and Sequential Kit Protocols
  • ProteoMiner Sequential Elution Kit Protocol
  • ProteoMiner Protein Enrichment Kits Instruction Manual
  • ProteoMiner Protein Enrichment Technology Brochure
  • Enrichment of Medium- and Low-Abundance Proteins in Sample Types Using ProteoMiner Technology Application Note