ReadyPrep™ 2-D Cleanup Kit for IEF, 50 preps #1632130

ReadyPrep™ 2-D Cleanup Kit for IEF, 50 preps

50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein

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Number Description Options
163-2130-MSDS ReadyPrep™ 2-D Cleanup Kit English

Use the ReadyPrep 2-D Cleanup Kit to remove contaminants and concentrate protein samples for isoelectric focusing (IEF), 2-D gel electrophoresis, and any other application where protein sample cleanup or concentration is needed. The kit improves 2D resolution and reproducibility.

  • Remove contaminants such as salts, lipids, detergents, nucleic acids and phenolic compounds
  • Concentrate proteins in dilute samples
  • Reduce streaking, background staining, and other artifacts
  • Clean up protein samples in any form; precipitation is unaffected by the presence of chaotropes, detergents, common buffers, salts and other reagents commonly used in protein preparations
  • Resuspend protein in any IEF/2DE-compatible or other rehydration/sample buffer

The TCA-like precipitation removes ionic contaminants from samples. Proteins are quantitatively precipitated and concentrated, with substances that can interfere with IEF or other applications being washed away.  Dilute samples can be concentrated for higher protein loads and enhanced spot detection. The isoelectric points of the proteins in the purified sample remain unaltered.

Product Contents

For 50 samples, each 100 µl containing up to 500 µg protein

  • Precipitation agent 1, 15 ml
  • Precipitation agent 2, 15 ml
  • Wash reagent 1, 2 ml
  • Wash reagent 2, 50 ml
  • Wash 2 additive, 0.25 ml
  • Instruction manual

Packaging Options

Number of Preps Catalog Number
5 1632140
50 1632130

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Supporting Documentation

Online Resources

2-D Electrophoresis and Analysis