ReadyPrep™ Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps #1632089

ReadyPrep™ Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps

50 preps, includes 50 ml cytoplasmic protein extraction buffer (CPEB), 50 ml protein solubilization buffer, 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 50 mg of cells or tissue

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Number Description Options
163-2089-MSDS ReadyPrep™ Protein Extraction Kit English

Use the ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) to enrich for both cytoplasmic and nuclear proteins. The kit separates cytoplasmic proteins from intact nuclei and then solubilizes nuclei.

  • Employs a specially formulated buffer and differential centrifugation to isolate intact nuclei
  • Contains a strongly chaotropic extraction buffer to efficiently solubilize nuclear proteins
  • Includes the components of the ReadyPrep 2-D Cleanup Kit to remove extraction buffer that may interfere with IEF
  • Extracted proteins are suitable for IEF, SDS-PAGE, and western blotting

The ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) uses a buffer/differential centrifugation protocol to generate enriched cytoplasmic fractions and intact nuclei (Dignam et al. 1983, Zerivitz and Akusjarvi 1989). The nuclei are treated with a strongly chaotropic extraction buffer to efficiently solubilize the nuclear proteins. The cytoplasmic fraction is processed with the included ReadyPrep 2-D Cleanup Kit to remove IEF-inhibiting compounds.  Although this kit is directed towards the preparation of nuclear and cytoplasmic protein samples for 2-D gel electrophoresis, the fractions are suitable for SDS-PAGE, western blotting, and other applications.

Kit Components

For 50 extractions of 50 mg of cells or tissue:

  • Cytoplasmic protein extraction buffer (CPEB), 50 ml
  • Protein solubilization buffer (PSB), 25 g
  • 3PSB diluent, 30 ml
  • ReadyPrep 2-D Cleanup Kit (1632130)
  • Instruction manual

Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.

ReadyPrep Kit for Fractionation by Cellular Location Proteins Targeted Catalog Number
ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) Cytoplasmic and nuclear 1632089
ReadyPrep Protein Extraction Kit (Membrane I) Membrane-anchored, with one or two transmembrane domains, and hydrophobic 1632088
ReadyPrep Protein Extraction Kit (Membrane II) More than two transmembrane domains and/or proteins resistant to Membrane I Kit 1632084
ReadyPrep Protein Extraction Kit (Signal) Associated with lipid rafts 1632087

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Supporting Documentation


Dignam JD et al. (1983). Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acid Res 11, 1475–1489. PMID: 6828386
Zerivitz K and Akusjarvi G (1989). An improved nuclear extract preparation method. Gene Anal Tech 6, 101–109. PMID: 2555292