Overview
50 preps, includes 50 ml signal protein extraction buffer 1 (S1), 50 ml signal protein extraction buffer 2 (S2), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, instruction manual; for 50 extractions of 25–50 mg of cells or tissue
Safety Data Sheet
Description
Description
Choose the ReadyPrep Protein Extraction Kit (Signal) to enrich for signaling proteins. This kit uses differential solubilization at 4°C in the presence of Triton X-100 to isolate proteins associated with lipid rafts.
- Isolate proteins involved in intracellular membrane trafficking and signaling pathways
- After fractionation, proteins are easily solubilized in the supplied buffer
- Solubilized proteins can be used immediately in IEF, SDS-PAGE and other applications
Many proteins located within lipid rafts are resistant to solubilization at 4°C by Triton X-100 and other nonionic detergents. The ReadyPrep Protein Extraction Kit (Signal) utilizes a proprietary mixture of nonionic detergents to extract and remove hydrophilic and soluble proteins, leaving behind the signal proteins in a detergent-insoluble fraction.
Proteins that can be isolated using this kit include
- Gycosylphosphatidylinositol (GPI)-anchored proteins
- Caveolin and other caveolae-associated proteins
- Acylated tyrosine kinases
- Heterotrimeric G-proteins
- Some proteins with transmembrane domains
Kit Components
For 50 extractions of 25–50 mg of cells or tissue:
- Signal protein extraction buffer 1 (S1), 50 ml
- Signal protein extraction buffer 2 (S2), 50 ml
- Protein solubilization buffer (PSB), 25 g
- PSB diluent, 30 ml
- Instruction manual
Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.
| ReadyPrep Kit for Fractionation by Cellular Location | Proteins Targeted | Catalog Number |
| ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) | Cytoplasmic and nuclear | 1632089 |
| ReadyPrep Protein Extraction Kit (Membrane I) | Membrane-anchored, with one or two transmembrane domains, and hydrophobic | 1632088 |
| ReadyPrep Protein Extraction Kit (Membrane II) | More than two transmembrane domains and/or proteins resistant to Membrane I Kit | 1632084 |
| ReadyPrep Protein Extraction Kit (Signal) | Associated with lipid rafts | 1632087 |
Related Products
- ReadyPrep Kits for Fractionation by Solubility
- Aurum™ Ion Exchange Mini Kits for Fractionation by Charge
- Rotofor® Liquid-Phase IEF Cells for Fractionation by pI
- Model 491 Prep Cell and Mini Prep Cell for Fractionation by Solubility
- ReadyPrep™ 2-D Starter Kit
- PROTEAN® i12™ IEF System
- ReadyStrip™ IPG Strips
- ReadyPrep Reduction-Alkylation Kit (1632090)
- PowerPac™ HV High-Voltage Power Supply
Related Categories
- Protein Gels
- Electrophoresis Chambers
- 2-D Electrophoresis Buffers and Reagents
- Ampholytes
- Tube Gel IEF Systems
- Gel Imaging Systems
- 2-D Electrophoresis Workflow
Supporting Documentation
- ReadyPrep Protein Extraction Kit (Signal) Instruction Manual
- Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure
- ReadyStrip IPG Strip Instruction Manual
- PROTEAN i12 IEF System Brochure
- Host Cell Protein (HCP) Workflow Quick Guide