2x TBE-Urea Sample Buffer, 30 ml #1610768


30 ml, 2x TBE-urea sample loading buffer, premixed, for denaturing ssDNA and RNA, pH 8.0

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Storage temperature 4°C
Shelf life 6 months at 4°C
Number Description Options
161-0768-MSDS TBE-Urea Sample Buffer English

Use 2x TBE-Urea Sample Buffer for denaturing electrophoresis of nucleic acids.

1x formulation: 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.0, 12% ficoll, 0.01% bromophenol blue, 0.02% xylene cyanole FF, 7 M urea

Instructions for use: Dilute sample 1:1 with sample buffer. Dry samples can be dissolved directly into the buffer. Heat at 70 °C for 4 min prior to loading. Temperatures up to 90 °C, may be required to completely denature some samples.

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