Native Sample Buffer for Protein Gels, 30 ml #1610738

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161-0738-MSDS Native Sample Buffer English

Use Native Sample Buffer to retain native protein structure and mass-to-charge ratios during protein electrophoresis. This sample buffer is nondenaturing, containing no SDS, and has no reducing agent. The mobility of proteins in native gels depends on a number of factors in addition to molecular weight, including protein shape and charge.

  • The biological activity of most proteins is retained
  • Some protein-protein interactions may be maintained
  • Native gel electrophoresis cannot be used for molecular weight estimation
  • Since migration is influenced by structure, proteins of the same size can often be easily resolved
  • Dry samples can be dissolved directly into the sample buffer

Precast gels that can be used for native electrophoresis

Precast Gels Size Gel Chemistry
Mini-PROTEAN® TGX™ Mini Laemmli-like, long shelf life, fast separation with high resolution
Criterion™ TGX™ Midi Laemmli-like, long shelf life, fast separation with high resolution
Mini-PROTEAN® TGX Stain-Free™ Mini Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection
Criterion™ TGX Stain-Free™ Midi Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection
Criterion™ Tris-HCl Midi Standard Laemmli
Criterion Stain Free™ Tris-HCl Midi Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection
Criterion XT Tris-Acetate Midi Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins


Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) are of interest, change the polarity of the electrodes, since they have a net positive charge.

 

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