Flamingo™ Fluorescent Protein Gel Stain, 500 ml #1610492

Flamingo™ Fluorescent Protein Gel Stain

500 ml, 10x Flamingo Fluorescent Gel Stain solution

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Compatible laser/emission filter Blue laser 473 nm/530 nm
Blue laser 488 nm/560 nm LP
Green laser 532 nm/555 nm LP
UV transilluminator Excitation centered on 300 nm
Storage Stable for at least 6 months when stored at 2–8°C, protected from light
Number Description Options
161-0490-MSDS Flamingo™ Fluorescent Gel Stain, 10X English

Choose economical, sensitive Flamingo Fluorescent Protein Gel Stain for the detection of denatured proteins in gels including first-dimension isoelectric focusing (IEF) gels. The dye is non-fluorescent in solution and becomes strongly fluorescent on binding to protein.

  • Linear response over 3 orders of magnitude, with a lower limit of 0.25–0.5 ng
  • No destaining required, and gels cannot be overstained
  • Low background; readily distinguish spots and speckles
  • Stain may be exposed to typical room light for up to 8 hours without affecting the fluorescence intensity of stained protein bands or spots
  • Stained gels may be stored in the dark at 2–8°C for up to 6 months without significant loss of imaging sensitivity

Flamingo Fluorescent Protein Gel Stain can be visualized with laser-based gel imaging systems or UV detectors (Ex 512 nm, Em 535 nm, secondary Ex 271 nm; see Specifications tab for laser/emission filter compatibility). The staining procedure is a simple two-step protocol that can be completed in as little as 5 hours. The steps are not time-sensitive. Stained proteins are compatible with mass spectrometry and enzymatic digestion.

Packaging Options

Flamingo Fluorescent Protein Gel Stain, Volume Catalog Number
20 ml 1610490
100 ml 1610491
500 ml 1610492

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Supporting Documentation

Online Resources

Staining and Visualization of Proteins After 2-D Electrophoresis