Precision Plus Protein™ WesternC™ Blotting Standards, 250 μl #1610376

Precision Plus Protein™ WesternC™ Standards

250 μl, 10 blue-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

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MW size range, kD 10–250
Stain type Combination of stained Dual Color and unstained
Volume, µl 250
Total number of applications 50
Shelf life at –20°C, months 12
Loading volume on mini gels, µl for chemiluminescent detection, 5. Precision Protein™ StrepTactin-HRP or -AP conjugate needed.
Loading volume on mini gels, µl for fluorescent blot detection, 5 of 1:10 dilution
Application Type  
Monitoring electrophoretic separation for SDS-PAGE gels Yes
Molecular weight sizing on SDS-PAGE gels Yes
Monitoring blot transfer efficiency Yes
Fluorescence blots Yes
Molecular weight sizing on Blots
Loading Volume on Mini Gels  
For Coomassie staining, µl N/A
For stain-free gels, µl N/A
For silver staining, µl N/A
For SYPRO Ruby staining, µl N/A
For blot development, µl N/A
StrepTactin conjugate working dilutions for colorimetric blot development N/A
StrepTactin conjugate working dilutions for chemiluminescence blot development N/A
Number Description Options
161-0376-MSDS Precision Plus™ Western C Protein Standards English

Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.

Applications and Uses

  • Monitoring electrophoretic separation
  • Monitoring blot transfer efficiency with easy orientation via multicolor standards
  • Molecular weight sizing of proteins after blot development
  • Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range

Packaging Options

Number of Applications (Volume)
Catalog Number
50 (250 μl) 1610376
250 (5 x 250 μl)

More Information

Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue.

Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.

Related Products

  • Precision Protein StrepTactin-HRP Conjugate, 300 µl (1610380)
  • Precision Protein StrepTactin-HRP Conjugate, 125 µl (1610381)
  • StrepTactin-AP Conjugate, 300 µl (1610382)

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