Flamingo™ Fluorescent Protein Gel Stain, 100 ml #1610491

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Overview

100 ml, 10x Flamingo Fluorescent Gel Stain solution
Safety Data Sheet

Safety Data Sheet

Description

Description

Choose economical, sensitive Flamingo Fluorescent Protein Gel Stain for the detection of denatured proteins in gels including first-dimension isoelectric focusing (IEF) gels. The dye is non-fluorescent in solution and becomes strongly fluorescent on binding to protein.

  • Linear response over 3 orders of magnitude, with a lower limit of 0.25–0.5 ng
  • No destaining required, and gels cannot be overstained
  • Low background; readily distinguish spots and speckles
  • Stain may be exposed to typical room light for up to 8 hours without affecting the fluorescence intensity of stained protein bands or spots
  • Stained gels may be stored in the dark at 2–8°C for up to 6 months without significant loss of imaging sensitivity

Flamingo Fluorescent Protein Gel Stain can be visualized with laser-based gel imaging systems or UV detectors (Ex 512 nm, Em 535 nm, secondary Ex 271 nm; see Specifications tab for laser/emission filter compatibility). The staining procedure is a simple two-step protocol that can be completed in as little as 5 hours. The steps are not time-sensitive. Stained proteins are compatible with mass spectrometry and enzymatic digestion.

Packaging Options

Flamingo Fluorescent Protein Gel Stain, Volume Catalog Number
20 ml 1610490
100 ml 1610491
500 ml 1610492

Related Products

Related Categories

Supporting Documentation

Online Resources

Staining and Visualization of Proteins After 2-D Electrophoresis