Precision Plus Protein™ Unstained Standards #161-0363

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Precision Plus Protein™ Unstained Standards

1 ml, unstained mixture of 10 Strep-tagged recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

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MW size range, kD 10–250
Stain type Unstained
Volume, µl 1,000
Total number of applications 100
Storage   Store at –20ºC until expiration date (24 months from date of manufacture)
Application Type  
Monitoring electrophoretic separation for SDS-PAGE gels N/A
Molecular weight sizing on SDS-PAGE gels Yes
Monitoring blot transfer efficiency N/A
Fluorescence blots N/A
Molecular weight sizing on Stain-free gels, blots
Loading Volume on Mini Gels  
For Coomassie staining, µl 10
For stain-free gels, µl 10
For silver staining, µl 1–3
For SYPRO Ruby staining, µl 1–3
For blot development, µl 1–10
StrepTactin conjugate working dilutions for colorimetric blot development N/A
StrepTactin conjugate working dilutions for chemiluminescence blot development N/A
Number Description Options
161-0363-MSDS Precision Plus Protein™ Unstained Standards English

The Precision Plus Protein™ Unstained standards are a mixture of ten Strep-tagged recombinant proteins (10–250 kD), including three reference bands (25, 50, and 75 kD). For blot development, Precision Protein™ StrepTactin-HRP conjugate (#161-0380) or Precision Protein StrepTactin-AP conjugate (#161-0382) should also be ordered.

Applications and Uses

Choose Precision Plus Protein Unstained standards for:

  • Molecular weight sizing on SDS-PAGE gels
  • Molecular weight sizing on stain-free gels
  • Molecular weight sizing on blots

More Information

Loading buffer:
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue

Store at –20ºC until expiration date (24 months from date of manufacture)