Precision Plus Protein™ Unstained Standards #161-0363

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Precision Plus Protein™ Unstained Standards

1 ml, unstained mixture of 10 Strep-tagged recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

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MW size range, kD 10–250
Stain type Unstained
Volume, µl 1,000
Total number of applications 100
Shelf life at –20°C, months 12
Application Type  
Monitoring electrophoretic separation for SDS-PAGE gels N/A
Molecular weight sizing on SDS-PAGE gels Yes
Monitoring blot transfer efficiency N/A
Fluorescence blots N/A
Molecular weight sizing on Stain-free gels, blots
Loading Volume on Mini Gels  
For Coomassie staining, µl 10
For stain-free gels, µl 10
For silver staining, µl 1–3
For SYPRO Ruby staining, µl 1–3
For blot development, µl 1–10
StrepTactin conjugate working dilutions for colorimetric blot development N/A
StrepTactin conjugate working dilutions for chemiluminescence blot development N/A
Number Description Options
161-0363-MSDS Precision Plus Protein™ Unstained Standards English

The Precision Plus Protein™ Unstained standards are a mixture of ten Strep-tagged recombinant proteins (10–250 kD), including three reference bands (25, 50, and 75 kD). For blot development, Precision Protein™ StrepTactin-HRP conjugate (#161-0380) or Precision Protein StrepTactin-AP conjugate (#161-0382) should also be ordered.

Applications and Uses

Choose Precision Plus Protein Unstained standards for:

  • Molecular weight sizing on SDS-PAGE gels
  • Molecular weight sizing on stain-free gels
  • Molecular weight sizing on blots

More Information

Loading buffer:
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue