Profinity™ IMAC Resin, uncharged, 10 ml #1560121EDU


Ligand Iminodiacetic acid (IDA)
Form 50% slurry in 20% ethanol, uncharged
Particle size 45–90 μm (mean 60 μm)
Capacity 12–30 µmol Cu2+/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein)
Maximum operating pressure 109 psi (7.5 bar)
Recommended flow rate ≤600 cm/hr at 25°C
Operating temperature range 4–40°C
Autoclavability (121°C, 30 min) Yes (in 0.1 M sodium acetate)
Storage 4°C or ambient
Shelf life ≥1 year at ambient temperature in 20% ethanol

Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. This resin can be used as part of the Protein Expression and Purification Series .

  • Chelating ligand is iminodiacetic acid (IDA)
  • Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
  • Stable across a wide pH range (1–14)
  • Works under native or denaturing conditions
  • Can be used for single-step purification from crude samples or contaminant removal

Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.

Related Products

  • Centrifugation Purification Module (1665041EDU) used in the third module purification option in Protein Expression and Purification Series Classroom Study Kit
  • Prepacked Bio-Scale™ Mini Profinity™ IMAC Cartridges with Ni-charged IDA, 5 x 1 ml (7324610EDU), 1 x 5 ml (7324612EDU), and 5 x 5 ml (7324614EDU)
  • Poly-Prep® Chromatography Columns (7311550EDU) hold up to 2ml support

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