Profinity™ IMAC Resin, uncharged, 10 ml #1560121

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Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

1560121 [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [54.224.234.8, 10.232.19.29] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml] [x-amzn-trace-id] = [Root=1-5ae2e42e-90c57f5fabb6917ca9a2909d] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1560121] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-us/sku/1560121-profinity-imac-resin-uncharged-10-ml] [connection] = [Keep-Alive] [accept-encoding] = [x-gzip, gzip, deflate] [user-agent] = [CCBot/2.0 (http://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1560121-profinity-imac-resin-uncharged-10-ml LSR en US en-us 07/30/13 03:25 AM en_US true internet/generic 2018-04-02 20:17:12 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1518242136325 2018-04-02 20:17:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 15 years, Bio-Rad has made science education a major priority. 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Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.</p> <ul> <li>Chelating ligand is iminodiacetic acid (IDA)</li> <li>Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>Works under native or denaturing conditions</li> <li>Can be used for single-step purification from crude samples or contaminant removal</li> </ul> <p>Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.</p> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="325" align="left" valign="top"><strong>Resin Form</strong></td> <td width="175" align="center" valign="top"><strong>Package Size </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560131-profinity-imac-resin-ni-charged-10-ml?ID=1560131">1560131</a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">25 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560133-profinity-imac-resin-ni-charged-25-ml?ID=1560133">1560133</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">100 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560135-profinity-imac-resin-ni-charged-100-ml?ID=1560135">1560135 </a></td> </tr> <tr> <td align="left" valign="top">Ni-charged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560137-profinity-imac-resin-ni-charged-500-ml?ID=1560137">1560137 </a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">10 ml</td> <td align="center" valign="top">1560121</td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">50 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560123-profinity-imac-resin-uncharged-50-ml?ID=1560123">1560123</a></td> </tr> <tr class="pd_colorbackground"> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">500 ml</td> <td align="center" valign="top"><a href="/en-us/sku/1560125-profinity-imac-resin-uncharged-500-ml?ID=1560125">1560125 </a></td> </tr> <tr> <td align="left" valign="top">Uncharged</td> <td align="center" valign="top">1 L</td> <td align="center" valign="top"><a href="/en-us/sku/1560127-profinity-imac-uncharged-resin">1560127</a></td> </tr> </tbody> </table> <p>Prepacked <a href="/en-us/product/bio-scale-mini-profinity-imac-cartridges">Bio-Scale&trade; Mini Profinity&trade; IMAC Cartridges</a> with uncharged or Ni-charged IDA, and <a href="/en-us/product/bio-scale-mini-nuvia-imac-cartridges">Bio-Scale&trade; Mini Nuvia&trade; IMAC Cartridges</a> with Ni-charged NTA are available.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Native IMAC Buffer Kit (<a href="/en-us/sku/6200239-native-imac-buffer-kit">6200239</a>) includes IMAC lysis, wash, and elution buffers</li> <li>Native IMAC Purification Kits, 1 ml (<a href="/en-us/sku/6200241-native-imac-purification-kit-1-ml">6200241</a>) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (<a href="/en-us/sku/6200242-native-imac-purification-kit-5-ml">6200242</a>) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge </li> <li><a href="/en-us/product/nuvia-imac-resin">Nuvia IMAC Resin</a>, Ni-charged nitrilotriacetic acid (NTA)</li> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> </a><a href="/en-us/product/poly-prep-chromatography-columns">Chromatography</a><a href="/en-us/product/poly-prep-chromatography-columns"> Columns</a>, hold up to 2 ml support</li> <li>His Antibody (<a href="/en-us/sku/6200203-his-antibody">6200203</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/transfection">Transfection</a></li> <li><a href="/en-us/category/spectrophotometry">Spectrophotometry</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity IMAC Resins <a href="/webroot/web/pdf/lsr/literature/10001677B.PDF">Instruction Manual</a></li> <li>Profinity IMAC Resin Product <a href="/webroot/web/pdf/lsr/literature/Bulletin_3193.pdf">Information Sheet</a></li> <li>Profinity IMAC Ni-Charged Resin <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5283.pdf" target="_blank">Flier</a></li> <li>Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5456.pdf" target="_blank">Tech Note </a></li> <li>Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/10005455.pdf" target="_blank">Instruction Manual</a></li> <li>Bio-Scale Mini Profinity IMAC Cartridge <a class="no-target-style" title="Link opens in new window" href="/webroot/web/pdf/lsr/literature/Bulletin_5444A.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/introduction-transfection">Introduction to Transfection</a></li> <li><a href="/en-us/applications-technologies/histidine-tagged-recombinant-protein-purification-column-refolding?ID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-imac-resin-uncharged-10ml-1560121.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-imac-resin-uncharged-10ml-1560121.html Ligand 1775 Iminodiacetic acid (IDA) 1 0 Form 1659 50% slurry in 20% ethanol, uncharged 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Capacity 1404 12–30 µmol Cu<sup>2+</sup>/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein) 1 0 Maximum operating pressure 1817 109 psi (7.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature in 20% ethanol 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560121&CartQuote=QT&quantity= false true false $87.00 false null 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Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.

  • Chelating ligand is iminodiacetic acid (IDA)
  • Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
  • Stable across a wide pH range (1–14)
  • Works under native or denaturing conditions
  • Can be used for single-step purification from crude samples or contaminant removal

Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.

Product Options

Resin Form Package Size Catalog Number
Ni-charged 10 ml 1560131
Ni-charged 25 ml 1560133
Ni-charged 100 ml 1560135
Ni-charged 500 ml 1560137
Uncharged 10 ml 1560121
Uncharged 50 ml 1560123
Uncharged 500 ml 1560125
Uncharged 1 L 1560127

Prepacked Bio-Scale™ Mini Profinity™ IMAC Cartridges with uncharged or Ni-charged IDA, and Bio-Scale™ Mini Nuvia™ IMAC Cartridges with Ni-charged NTA are available.

Related Products

  • Native IMAC Buffer Kit (6200239) includes IMAC lysis, wash, and elution buffers
  • Native IMAC Purification Kits, 1 ml (6200241) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (6200242) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge
  • Nuvia IMAC Resin, Ni-charged nitrilotriacetic acid (NTA)
  • Empty Bio-Scale Mini Cartridges, 1 ml (7324660) and 5 ml (7324661)
  • Poly-Prep® Chromatography Columns, hold up to 2 ml support
  • His Antibody (6200203)

Related Categories

Supporting Documents

Online Resources

Ligand Iminodiacetic acid (IDA)
Form 50% slurry in 20% ethanol, uncharged
Particle size 45–90 μm (mean 60 μm)
Capacity 12–30 µmol Cu2+/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein)
Maximum operating pressure 109 psi (7.5 bar)
Recommended flow rate ≤600 cm/hr at 25°C
Operating temperature range 4–40°C
Autoclavability (121°C, 30 min) Yes (in 0.1 M sodium acetate)
Storage 4°C or ambient
Shelf life ≥1 year at ambient temperature in 20% ethanol
Available Translation
Profinity™ IMAC Uncharged Resin  (SDS)gene

Bulletin# 156-0121-MSDS

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