PureBlu™ DAPI Nuclear Staining Dye #1351303

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Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder

1351303 [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [66.160.140.183, 10.232.18.50] [seourl] = [/en-us/sku/1351303-pureblu-dapi-nuclear-staining-dye] [x-amzn-trace-id] = [Root=1-5c4834cf-707d1cd59b7ba8eac96b0802] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1351303] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/sku/1351303-pureblu-dapi-nuclear-staining-dye] [from] = [the.knowledge.ai@gmail.com] [connection] = [Keep-Alive] [accept-encoding] = [gzip,deflate] [user-agent] = [The Knowledge AI] Catalog/ProductSKU pageStyleKey 1351303-pureblu-dapi-nuclear-staining-dye LSR en US en-us /prd 07/30/13 03:25 AM en_US true internet/generic 2018-04-02 20:17:12 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1518242136325 2018-04-02 20:17:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 15 years, Bio-Rad has made science education a major priority. 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PureBlu DAPI Dye is a highly pure formulation of DAPI in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.</p> <p>DAPI (4',6-diamidino-2-phenylindole) is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. DAPI permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).</p> <p><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/molecular-binding-dapi.jpg" alt="DAPI binding dsDNA" /></p> <p class="caption"><strong>Fig. 1. DAPI binding dsDNA.</strong><br />&nbsp;</p> <p>When bound to dsDNA, PureBlu DAPI Dye has a maximum excitation wavelength in the ultraviolet range (359 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for DAPI, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.</p> <p><br /><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/dapi-emission-spectrum.gif" alt="DAPI emission spectrum" /></p> <p class="caption"><strong>Fig. 2. DAPI excitation and emission spectra.</strong><br />&nbsp;</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imaging System</a></li> <li><a href="/en-us/product/tc20-automated-cell-counter?ID=M7FBG34VY">TC20&trade; Automated Cell Counter</a></li> </ul> <h2 class="descHeader">More Information</h2> <p><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10043282.pdf" target="_blank" rel="noopener noreferrer">PureBlu&trade; DAPI Nuclear Staining Dye Product Insert</a></p> /webroot/web/html/lsr/products/SKUs 135-1303.html /webroot/web/html/lsr/products/SKUs/135-1303.html Molecular formula 2963 C<sub>16</sub>H<sub>15</sub>N<sub>5</sub>·2HCl 1 0 Molecular weight 2964 350.25 1 0 Excitation/emission wavelengths 2897 359/461 nm 1 0 CAS Number 2961 28718-90-3 1 0 Purity 2036 >95% (HPLC purified) 1 0 Solubility 2962 Soluble in water and dimethyl sulfoxide (DMSO) 1 0 Storage and use 2877 Long-term storage at –20ºC; protect from light 1 0 Temperature stability 2251 Stable for 2 years at –20°C <br />After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C 1 0 1351304 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml 1450031 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/ZOE-Fluorescent-Cell-Imager/145-0031_1403030204.xml false /en-us/life-science-research/promotions/20-off-nuclear-staining-dyes false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351303&CartQuote=QT&quantity= 1108 false true false $116.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351303&AddToDefaultWishList=true&quantity= true false true 12171500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351303&quantity= 135-1303-MSDS PureBlu™ DAPI Nuclear Staining Dye Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_1351303.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_1351303.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_1351303.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_1351303.pdf Mexico Spanish /webroot/web/pdf/WWMSDS/LSGC/MEX/MEX_ES_1351303.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_1351303.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_1351303.pdf Russian Federation Russian /webroot/web/pdf/WWMSDS/LSGC/RUS/RUS_RUS_1351303.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_1351303.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_1351303.pdf Brazil Portuguese /webroot/web/pdf/WWMSDS/LSGC/BRA/BRA_PT_1351303.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_1351303.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_1351303.pdf United Arab Emirates English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_1351303.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_1351303.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_1351303.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_1351303.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_1351303.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_1351303.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_1351303.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_1351303.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_1351303.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_1351303.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_1351303.pdf true China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_1351303.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_1351303.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_1351303.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_1351303.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_1351303.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_1351303.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_1351303.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_1351303.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_1351303.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_1351303.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_1351303.pdf Italy English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_1351303.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_1351303.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_1351303.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_1351303.pdf Finland Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_1351303.pdf Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_1351303.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_1351303.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_1351303.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_1351303.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_1351303.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_1351303.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_1351303.pdf
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Use PureBlu DAPI Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu DAPI Dye is a highly pure formulation of DAPI in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.

DAPI (4',6-diamidino-2-phenylindole) is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. DAPI permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).

DAPI binding dsDNA

Fig. 1. DAPI binding dsDNA.
 

When bound to dsDNA, PureBlu DAPI Dye has a maximum excitation wavelength in the ultraviolet range (359 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for DAPI, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.


DAPI emission spectrum

Fig. 2. DAPI excitation and emission spectra.
 

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PureBlu™ DAPI Nuclear Staining Dye Product Insert

Molecular formula C16H15N5·2HCl
Molecular weight 350.25
Excitation/emission wavelengths 359/461 nm
CAS Number 28718-90-3
Purity >95% (HPLC purified)
Solubility Soluble in water and dimethyl sulfoxide (DMSO)
Storage and use Long-term storage at –20ºC; protect from light
Temperature stability Stable for 2 years at –20°C
After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C
Number Description Options
Available Translation
PureBlu™ DAPI Nuclear Staining Dye  (SDS)gene

Bulletin# 135-1303-MSDS

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