ReadiDrop™ 7-AAD #135-1102

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Use ReadiDrop 7-AAD (7-aminoactinomycin D), a ready-to-use cell viability dye, for excluding dead cells in flow cytometric experiments. As a cell-impermeable assay, ReadiDrop 7-AAD enters a compromised cell and binds to double-stranded DNA with a high-affinity intercalation between GC base pairs.

The assay removes the traditional manual weighing, pipetting, and dilution steps when using commonly available cell viability dyes in flow cytometry experiments. Simply add 1 or 2 drops of the cell viability assay to assess the health of your cells.

Key Benefits of the ReadiDrop 7-AAD Cell Viability Dye

  • Ideal for cell sorting — fluorescent molecules are suspended in PBS without preservatives
  • Stable at room temperature — keep a bottle of any ReadiDrop 7-AAD cell viability assay by your cell sorter or cell analyzer for easy access
  • No more weighing, pipetting, and diluting — ready-to-use solution gives 1 µg/ml as the final concentration

Features of the ReadiDrop 7-AAD Cell Viability Dye

  • Fluorophore Properties
    • Excitation maximum: 548 nm
    • Emission maximum: 648 nm
    • Optimal excitation laser: 561 nm or 488 nm
  • Fluorescent Spectrum

 Fluorescent Spectrum

  • Molecular weight: 1270.43 g/mol
  • Molecular formula: C62H87N13O16
  • Chemical structure

Chemical Structure

  • Specifications
    • Store at 15–30°C
    • Do not freeze

Related Products

Design your custom flow cytometric experiment with our available fixable viability dyes:

VivaFix™ Cell Viability Assays Excitation Maximum, nm Emission Maximum, nm Optimal Excitation Laser, nm Optimal Filter Set for S3™ 488/561 nm Cell Sorter Catalog Number
VivaFix 353/442 353 442 355 135-1111
VivaFix 410/450 410 450 405 135-1112
VivaFix 408/512 408 512 405 135-1113
VivaFix 398/550 398 550 405 135-1114
VivaFix 498/521 498 521 488 FL1 (525/30) 135-1115
VivaFix 547/573 547 573 561 FL2 (586/25) 135-1116
VivaFix 583/603 583 603 561 FL3 (615/25) 135-1117
VivaFix 649/660 649 660 640 135-1118
 
ReadiDrop Cell Viability Assays Excitation Maximum, nm Emission Maximum, nm Optimal Excitation Laser, nm Optimal Filter Set for S3 488/561 nm Cell Sorter Catalog Number
ReadiDrop propidium iodide 538 617 488 FL3 (585/25) 135-1101
ReadiDrop 7-AAD 548 648 488 FL4 (655LP) 135-1102

Additional Products for Flow Cytometry

Bio-Rad also offers reagents and consumables to complete each step of flow cytometry experiments:

  • Propidium iodide solution — assess cell health with no weighing, pipetting, or dilution steps
  • Cell proliferation assays — easily incorporate 1 of 5 cell proliferation dyes into your multicolor experiment to monitor cell health or cell division
  • Antibody labeling kits — label your antibody of interest in 2 easy steps without a purification column
  • Primary antibodies — use high-quality monoclonal and polyclonal primary antibodies for applications ranging from stem cell, immunology, and cell biology to veterinary research
  • Fixable viability dyes — easily introduce a viability assay in your multicolor experiments

More Information

Learn more about ReadiDrop™ 7-AAD solution  and the features of the S3™ cell sorter.