ReadiDrop™ Propidium Iodide #135-1101

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9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion

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Use ReadiDrop propidium iodide, a ready-to-use cell viability dye, for excluding dead cells in flow cytometry experiments. As a cell-impermeable assay, ReadiDrop propidium iodide enters a compromised cell and binds to double-stranded DNA/RNA by intercalating between base pairs.

Easily combine this labeling dye with your GFP experiment to exclude dead cells. Detect the results in FL3 (585/25) of the 488/561 S3™ cell sorter system.

Key Benefits of ReadiDrop Propidium Iodide Cell Viability Assay

  • Ideal for cell sorting — fluorescent molecules are suspended in PBS without preservatives
  • Stable at room temperature — keep a bottle of ReadiDrop propidium iodide cell viability assay by your cell sorter or cell analyzer for easy access
  • No more weighing, pipetting, or diluting — ready-to-use solution to gives 1 µg/ml as the final concentration

Features of the ReadiDrop Propidium Iodide Cell Viability Dye

  • Fluorophore Properties
    • Excitation maximum: 538 nm
    • Emission maximum: 617 nm
    • Optimal excitation laser: 561 nm or 488 nm
  • Fluorescent Spectrum

Fluorescent Spectrum

  • Molecular weight: 668.39 g/mol
  • Molecular formula: C27H34I2N4
  • Chemical structure

Chemical Structure

  • Specifications
    • Store at 15–30°C
    • Do not freeze

Related Products

Design your custom flow cytometric experiment with our available fixable viability dyes:

VivaFix™ Cell Viability Assays Excitation Maximum, nm Emission Maximum, nm Optimal Excitation Laser, nm Optimal Filter Set for S3™ 488/561 nm Cell Sorter Catalog Number
VivaFix 353/442 353 442 355 135-1111
VivaFix 410/450 410 450 405 135-1112
VivaFix 408/512 408 512 405 135-1113
VivaFix 398/550 398 550 405 135-1114
VivaFix 498/521 498 521 488 FL1 (525/30) 135-1115
VivaFix 547/573 547 573 561 FL2 (586/25) 135-1116
VivaFix 583/603 583 603 561 FL3 (615/25) 135-1117
VivaFix 649/660 649 660 640 135-1118
 
ReadiDrop Cell Viability Assays Excitation Maximum, nm Emission Maximum, nm Optimal Excitation Laser, nm Optimal Filter Set for S3 488/561 nm Cell Sorter Catalog Number
ReadiDrop propidium iodide 538 617 488 FL3 (585/25) 135-1101
ReadiDrop 7-AAD 548 648 488 FL4 (655LP) 135-1102

Additional Products for Flow Cytometry

Bio-Rad also offers reagents and consumables to complete each step of flow cytometry experiments:

  • 7-AAD solution — assess cell health with no weighing, pipetting, and dilution steps
  • Cell proliferation assays — easily incorporate 1 of 5 cell proliferation dyes into your multicolor experiment to monitor cell health or cell division
  • Antibody labeling kits — label your antibody of interest in 2 easy steps without a purification column
  • Primary antibodies — use high-quality monoclonal and polyclonal primary antibodies for applications ranging from stem cell, immunology, cell biology to veterinary research
  • Fixable viability dyes — easily introduce a viability assay in your multicolor experiments

More Information

Learn more about ReadiDrop™ propidium iodide solution  and the features of the S3™ cell sorter.