High-resolution melting curve analysis to establish CYP2C19â2 single nucleotide polymorphism: Comparison with hydrolysis SNP analysis.
Manna M TemesvÃ¡ri, JÃ³zsef J Paulik, LÃ¡szlÃ³ L KÃ³bori, Katalin K Monostory
Molecular and Cellular Probes
Clinically significant consequences of CYP2C19 polymorphism in drug metabolism require reliable genotyping methods for assaying large numbers of blood samples. Hydrolysis single nucleotide polymorphism analysis and high-resolution melting curve (HRM) analysis successfully genotyped the 114 liver donors for CYP2C19â2. However, the HRM analysis was found to be more efficient, rapid, user- and cost-friendly method.