Examination of AKAP12 promoter methylation in skin cancer using methylation-sensitive high-resolution melting analysis.
W W Wu, J J Zhang, H H Yang, Y Y Shao, B B Yu
Clinical and Experimental Dermatology
A kinase anchor protein 12 (AKAP12/gravin) belongs to a family of scaffold proteins and organizes protein kinase (PK)A and PKC. DNA hypermethylation in the AKAP12 promoter region has been reported in a variety of human cancers with the exception of skin cancer. Methylation-specific high-resolution melting (MS-HRM) analysis is a novel tool for analysis of promoter methylation.