qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.

print

Jan Hellemans, Geert Mortier, Anne De Paepe, Frank Speleman, Jo Vandesompele

Genome biology

2007-01-01

Abstract:

Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.



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