Zeta-Probe Membranes

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Overview

Specifications
Membrane Binding Capacity (µg/cm2) Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot PVDF 150–160 Immunoblotting
Sequi-Blot PVDF 170–200 Protein sequencing
Zeta-Probe membrane 150 DNA/RNA hybridization
Zeta-Probe GT membrane 150 DNA/RNA hybridization
C/P Lift membrane 150 Colony/plaque screening
     
Guide to Nucleic Acid Blotting Membranes

To fulfill multiple blotting requirements and experimental designs, Bio-Rad offers a comprehensive line of nucleic acid blotting matrices, sold in rolls or in precut sheets to fit the Model 785 vacuum blotter.

Membrane Binding Capacity (µg/cm2) Detection Capability (pg) Major Applications
Supported nitrocellulose 80–100 5–50 DNA/RNA hybridization
Zeta-Probe membrane 150.0 1.0 DNA/RNA hybridization
Zeta-Probe GT membrane 150.0 1.0 DNA/RNA hybridization
C/P Lift membrane 150.0 1.0 Colony and plaque screening
 
Electrophoresis Apparatus Blotting Apparatus Precut Membranes Precut Filter Paper
Sub-Cell GT cell Vacuum blotter 15 x 20 cm
15 x 15 cm
15 x 9.2 cm
15 x 20 cm
Wide Mini-Sub GT cell Vacuum blotter 10 x 15 cm 14 x 16 cm

Zeta-Probe® membranes are high-tensile-strength cationized nylon membranes that carry a high-density quaternary amine charge. These pliable, flexible membranes are resistant to shrinking, tearing, and becoming brittle during transfer, hybridization, or reprobing procedures.

Zeta-Probe membranes bind nucleic acids independently of buffer pH, so they can be used in traditional Southern blots, rapid alkaline Southern or northern blotting techniques, and electrophoretic transfer of nucleic acids from agarose and polyacrylamide gels.

Zeta-Probe membranes can be repeatedly hybridized and stripped at 100°C in 0.5% SDS, 0.1x SSC as many as 20 times for DNA (Li et al. 1987) and 6 times for RNA (Gatti et al. 1984). Oligonucleotides as short as 6 bases will bind to the membrane, and oligonucleiotides ≥ 20 bases long will be retained after repeated hybridization and washing.

References

Gatti RA et al. (1984). Multiple uses of Southern blots. Biotechniques 2, 148-155.

Li JK et al. (1987). Rapid alkaline blot-transfer of viral dsRNAs. Anal Biochem 163, 210-218.

Zeta-Probe Membrane

162-0153
Pkg of 15, 9 x 12 cm, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $158.00
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Zeta-Probe Membrane

162-0154
Pkg of 15, 10 x 15 cm, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $184.00
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Zeta-Probe Membrane

162-0155
Pkg of 15, 15 x 15 cm, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $205.00
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Zeta-Probe Membrane

162-0156
Pkg of 15, 15 x 20 cm, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $276.00
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Zeta-Probe Membrane

162-0157
Pkg of 15, 20 x 20 cm, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $363.00
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Zeta-Probe Membrane

162-0158
Pkg of 3, 20 x 25 cm, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $117.00
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Zeta-Probe Membrane

162-0159
Pkg of 1 roll, 30 cm x 3.3 m, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $399.00
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Zeta-Probe Membrane

162-0165
Pkg of 1 roll, 20 cm x 3.3 m, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $297.00
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Zeta-Probe Membrane

162-0206
Pkg of 15, 7 x 10 cm, high-strength nylon membrane positively charged with quaternary amine groups

List Price:   $143.00
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Number Description Options
1110 Zeta-Probe Blotting Membranes for Northern, Southern, Alkaline Southern, and Western Blotting Bulletin, Rev A Click to download
LIT234 Instruction Manual, Zeta-Probe Blotting Membranes, Rev C Click to download