Zeta-Probe GT Membranes

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Overview

Specifications
Membrane Binding Capacity (µg/cm2) Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot PVDF 150–160 Immunoblotting
Sequi-Blot PVDF 170–200 Protein sequencing
Zeta-Probe membrane 150 DNA/RNA hybridization
Zeta-Probe GT membrane 150 DNA/RNA hybridization
C/P Lift membrane 150 Colony/plaque screening
     
Dilution Series

Dilution series with a standard Zeta-Probe GT control membrane. Human DNA (≥50 kb) was digested to completion with BglI. Samples of 7.5, 5, 2.5, 1, 0.75, 0.5, and 0.25 µg DNA from the enzyme digest were electrophoresed in a 1% agarose TBE gel, then transferred onto Zeta-Probe GT membrane. Following hybridization with a probe (provided by the Genetics Institute of Boston) to factor VIII gene, the blotted membrane was washed and exposed overnight to Kodak XAR film.

Guide to Nucleic Acid Blotting Membranes

To fulfill multiple blotting requirements and experimental designs, Bio-Rad offers a comprehensive line of nucleic acid blotting matrices, sold in rolls or in precut sheets to fit the Model 785 vacuum blotter.

Membrane Binding Capacity (µg/cm2) Detection Capability (pg) Major Applications
Supported nitrocellulose 80–100 5–50 DNA/RNA hybridization
Zeta-Probe membrane 150.0 1.0 DNA/RNA hybridization
Zeta-Probe GT membrane 150.0 1.0 DNA/RNA hybridization
C/P Lift membrane 150.0 1.0 Colony and plaque screening
 
Electrophoresis Apparatus Blotting Apparatus Precut Membranes Precut Filter Paper
Sub-Cell GT cell Vacuum blotter 15 x 20 cm
15 x 15 cm
15 x 9.2 cm
15 x 20 cm
Wide Mini-Sub GT cell Vacuum blotter 10 x 15 cm 14 x 16 cm

Zeta-Probe GT (genomic DNA-tested) membranes are designed for sensitive genomic applications. Like the original Zeta-Probe membranes, these are high-tensile-strength cationized nylon membranes that carry a high-density quaternary amine charge. These pliable, flexible membranes are resistant to shrinking, tearing, and becoming brittle during transfer, hybridization, or reprobing procedures.

Zeta-Probe GT membranes bind nucleic acids independently of buffer pH, so they can be used in rapid alkaline Southern or northern blotting techniques as well as in traditional Southern blots. The membranes bind nucleic acids in low-ionic-strength buffers, making electrophoretic transfer of nucleic acids from agarose and acrylamide gels possible. Oligonucleotides as short as 6 bases will bind the membrane through spot application, and those at least 20 bases long will be retained after repetitive hybridization and washing steps.

Every lot of Zeta-Probe GT membranes is functionally tested to ensure that 3 pg of target single-copy factor VIII human DNA can be detected in 5 µg total genomic DNA using a radiolabeled probe. The blots are produced in two different ways prior to hybridization: a standard high-salt (10x SSC) Southern transfer, and a 4 hour alkaline transfer (0.4 N NaOH). In the quality control procedure, 5 and 10 µg of digested human genomic DNA are probed with a factor VIII cDNA clone (single-copy gene probe). This corresponds to actual target sizes of only 1.5 and 3.0 pg of DNA, respectively, which are visualized in an overnight exposure. Bio-Rad quality control procedures also include a 3 day exposure to check for nonspecific background. Zeta-Probe GT membranes are accompanied by a performance certificate that illustrates the lot-specific results and includes an outline of the experiments and a photograph of the original autoradiogram.

References

Gatti RA et al. (1984). Multiple uses of Southern blots. Biotechniques 2, 148-155.

Li JK et al. (1987). Rapid alkaline blot-transfer of viral dsRNAs. Anal Biochem 163, 210-218.

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Zeta-Probe GT Membrane

1620190
Pkg of 15, 9 x 12 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$190.00
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Zeta-Probe GT Membrane

1620191
Pkg of 15, 10 x 15 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$215.00
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Zeta-Probe GT Membrane

1620192
Pkg of 15, 15 x 15 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$305.00
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Zeta-Probe GT Membrane

1620193
Pkg of 15, 15 x 20 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$375.00
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Zeta-Probe GT Membrane

1620194
Pkg of 15, 20 x 20 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$470.00
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Zeta-Probe GT Membrane

1620195
20 x 20 cm, 3 sheets, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$150.00
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Zeta-Probe GT Membrane

1620196
Pkg of 1 roll, 30 cm x 3.3 m, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$485.00
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Zeta-Probe GT Membrane

1620197
Pkg of 1 roll, 20 cm x 3.3 m, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

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$390.00
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(Discontinued) Zeta-Probe GT Membrane

1620208
Pkg of 15, 7 x 10 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups

This product is no longer available. Please contact technical support for more information on replacement product.
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1645 Zeta-Probe® GT Membranes Product Information Sheet Click to download