V3 Western Workflow™

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V3 Western Workflow - Western Blotting

Overview

V3 Western Workflow™
Tutorial: V3 Western Workflow™ with Stain-Free Imaging

Bio-Rad's V3 Western Workflow provides researchers with more confidence in the blotting process and in their results. This video demonstrates the fast, convenient, and transparent V3 Western Workflow process, how Image Lab™ software performs data normalization using total protein loading controls, and how stain-free gel technology is used to yield more reliable western blot results.


 
Webinar: Can We Trust Western Blots?

In this 45 min presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.


 
Using Image Lab™ Software for Total Protein Normalization on Western Blots
Webinar: Single-Cell Western Blot and Stain-Free Total Protein Loading Control

Robyn Murphy, Associate Professor at the School of Life Sciences of Latrobe University, has developed a unique protocol to conduct western blotting on muscles at single-cell levels to examine fiber type-specific protein expression patterns. Find out how Bio-Rad's stain-free gel technology helps ensure quantitative analysis in this unique procedure.


 
Mini-PROTEAN® TGX Stain-Free™ Precast Gel Ordering Information
  Mini-PROTEAN TGX Stain-Free Precast Gels
  8+1-Well 30 µl 10-Well 30 µl 10-Well 50 µl
12-Well 20 µl
15-Well 15 µl
IPG/Prep Well 7 cm IPG Strip 450 µl
 
Resolving
Gel %
8+1-Well
30 µl
10-Well
30 µl
10-Well
50 µl
12-Well
20 µl
15-Well
15 µl
IPG/Prep Well
7 cm IPG Strip
450 µl
 
7.5%
4568029 4568023 4568024 4568025 4568026 4568021
10%
4568039 4568033 4568034 4568035 4568036 4568031
12%
4568049 4568043 4568044 4568045 4568046 4568041
4–15% 4568089 4568083 4568084 4568085 4568086 4568081
4–20% 4568099 4568093 4568094 4568095 4568096 4568091
8–16% 4568109 4568103 4568104 4568105 4568106 4568101
Any kD™
4568129 4568123 4568124 4568125 4568126 4568121
 
Criterion™ TGX Stain-Free™ Precast Gel Ordering Information
  Criterion TGX Stain-Free Gels
  12+2-Well 45 µl 18-Well 30 µl 26-Well 15 µl Prep+2-Well 800 µl IPG+1-Well 11 cm IPG Strip
 
Resolving Gel % 12+2-Well
45 µl
18-Well
30 µl
26-Well
15 µl
Prep+2-Well
800 µl
IPG+1-Well
11 cm IPG Strip
 
7.5% 5678023 5678024 5678025
10% 5678033 5678034 5678035
12% 5678043 5678044 5678045
18% 5678073 5678074 5678075 5678072 5678071
4–15% 5678083 5678084 5678085 5678082 5678081
4–20% 5678093 5678094 5678095 5678092 5678091
8–16% 5678103 5678104 5678105 5678102 5678101
10–20% 5678113 5678114 5678115 5678112 5678111
Any kD™ 5678123 5678124 5678125 5678122 5678121
 


Set up a live demo with a Bio-Rad Imaging Specialist

V3 Workflow
 
What Is Stain-Free Technology?
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Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantitating proteins.

The complete V3 Western Workflow incorporates traditional blotting techniques with innovative tools, such as TGX Stain-Free™ Precast Gels, the Trans-Blot® Turbo™ Transfer System, and the ChemiDoc™ Touch Imaging System, to quickly check electrophoresis results and blot transfer quality prior to western blotting. This simplifies total protein quantitation analysis post-immunodetection and obtains results faster.

5 Steps

  1. Separate proteinsMini-PROTEAN® TGX Stain-Free™ Precast Gels and Criterion™ TGX Stain-Free™ Precast Gels offer superior protein separation with fast run times and use standard Tris/glycine running buffers. TGX Stain-Free Precast Gels contain a unique technology formulated into the gel chemistry that enables rapid, high-quality separation of proteins in as little as 15 minutes.
  2. Visualize proteins — protein separation can be visualized and confirmed using stain-free technology, which covalently binds proteins, after a 1-minute activation on a ChemiDoc Touch or ChemiDoc™ MP Imager. This allows the immediate visualization of proteins across the whole gel without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow.
  3. Transfer proteins — the Trans-Blot Turbo System is a rapid protein transfer apparatus that can reduce transfer protocols to as little as 3 minutes while maintaining high-efficiency protein transfers across a wide range of molecular weights.
  4. Verify protein transfer — the ChemiDoc Touch and ChemiDoc MP Imagers, paired with stain-free technology, enable instant verification of protein transfer.
  5. Validate and quantitate — the fifth step, available only with a ChemiDoc Touch or ChemiDoc MP Imager and Image Lab™ Software, may be used to validate western blotting data via total protein normalization. When normalizing to total protein, stripping and reprobing is no longer necessary. Image Lab Software gives quantitative results instead of relative normalization.

V3 Western Workflow

The V3 Western Workflow is available in six different configurations:

V3 Western Workflow Complete System Configuration Guide
 
ChemiDoc Touch Imaging System
ChemiDoc MP System
Gel Doc EZ System
Imager ChemiDoc Touch Imaging System ChemiDoc MP System Gel Doc™ EZ System
Detection type Chemiluminescence and routine protein and DNA gel imaging Chemiluminescence, multiplex fluorescence, and routine protein and DNA gel imaging Routine protein and DNA gel imaging
Western blot sensitivity +++++ ++++ N/A
Stain-free technology Yes Yes Yes
Touch screen/ embedded computer Yes No No
Gel format Mini precast gels Midi precast gels Mini precast gels Midi precast gels Mini precast gels Midi precast gels
Catalog number 170-8381 170-8382 171-8292 171-8293 170-8302 170-8303

More Information

View the Imaging System Selection Guide to find the best imager for your lab.

Contact a specialist to discuss your imaging needs.

Related Products

Browse western blotting products and protein gels.

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ChemiDoc Touch V3 Western Workflow for Mini Gels
ChemiDoc™ Touch V3 Western Workflow™ for Mini Gels

1708381
ChemiDoc Touch Imaging System, Image Lab™ Touch Software, Chemi/UV/Stain-Free Sample Tray, 50 Any kD™ Mini-PROTEAN® TGX Stain-Free™ Precast Gels, SDS-PAGE accessories, Clarity™ Substrate, Precision Plus Protein™ Standards, Mini-PROTEAN Tetra Cell, Trans-Blot® Turbo™ Transfer System Starter Kit, 50 PVDF transfer packs for mini gels

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ChemiDoc Touch V3 Western Workflow for Midi Gels
ChemiDoc™ Touch V3 Western Workflow™ for Midi Gels

1708382
ChemiDoc Touch Imaging System, Image Lab™ Touch Software, Chemi/UV/Stain-Free Sample Tray, 50 4–20% Criterion™ TGX Stain-Free™ Precast Gels, SDS-PAGE accessories, Clarity™ Substrate, Precision Plus Protein™ Standards, Criterion™ Cell, Trans-Blot® Turbo™ Transfer System Starter Kit, 50 PVDF transfer packs for midi gels

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V3 Western Workflow™ Complete System for Mini Gels

1708292
ChemiDoc™ MP Imager, Image Lab™ Software, 50 Any kD Mini-PROTEAN® TGX Stain-Free™ Precast Gels, SDS-PAGE accessories, Mini-PROTEAN Tetra cell, Trans-Blot® Turbo™ Transfer System Starter Kit, 50 PVDF transfer packs for mini gels

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V3 Western Workflow™ Complete System for Midi Gels

1708293
ChemiDoc™ MP Imager, Image Lab™ Software, 50 4–20% Criterion™ TGX Stain-Free™ Precast Gels, SDS-PAGE accessories, Clarity™ Substrate, Precision Plus Protein™ Standards, Criterion Cell, Trans-Blot® Turbo™ Transfer System Starter Kit, 50 PVDF transfer packs for midi gels

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V3 Western Workflow™ with Gel Doc™ EZ System for Mini Gels

1708302
Gel Doc EZ Imager with sample tray, Image Lab™ Software, 20 4–20% Mini-PROTEAN® TGX Stain-Free™ Precast Gels, SDS-PAGE accessories, Mini-PROTEAN Tetra Cell, Trans-Blot® Turbo™ Transfer System Starter Kit, 20 PVDF transfer packs for mini gels

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V3 Western Workflow™ with Gel Doc™ EZ System for Midi Gels

1708303
Gel Doc EZ Imager with sample tray, Image Lab™ Software, 20 4–20% Criterion™ TGX Stain-Free™ Precast Gels, SDS-PAGE accessories, Criterion Cell, Trans-Blot® Turbo™ Transfer System Starter Kit, 20 PVDF transfer packs for midi gels

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Number Description Options
6517 ChemiDoc™ Touch Imaging System Brochure, Ver D Click to download [ Add to Cart (Free) ]
6570 Experimental Protocol for Multiplex Fluorescent Blotting Using the ChemiDoc™ MP Imaging System, Rev A Click to download
6216 Detection Buffer Formulations Protocol, Rev A Click to download
6218 Blot Stripping and Reprobing Protocol, Rev A Click to download
6427 Bio-Rad V3 Western Workflow, Rev B Click to download [ Add to Cart (Free) ]
6434 Western Blot Normalization Using Image Lab™ Software, Quick Start Guide, Rev B Click to download [ Add to Cart (Free) ]
6253 V3 Western Workflow™ with ChemiDoc™ MP Imager Brochure, Rev C Click to download [ Add to Cart (Free) ]
6088 V3 Western Workflow with Gel Doc EZ Imager Brochure, Rev B Click to download
6264 V3 Western Workflow Protocol Customer Sample, Rev B Click to download [ Add to Cart (Free) ]
RP0051 Stain-Free Approach for Western Blotting Click to download
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
6133 ChemiDoc™ MP Brochure, Rev C Click to download [ Add to Cart (Free) ]
6039 Trans-Blot® Turbo™ Transfer System Brochure, Rev E Click to download [ Add to Cart (Free) ]
6166 Multiplex Fluorescent Blot Detection: A Troubleshooting Guide, Rev A Click to download [ Add to Cart (Free) ]
6349 Total Protein Normalization Publication List, Rev B Click to download
6350 Housekeeping Protein Normalization Publications, Rev A Click to download
6351 Publication List: Total Protein Normalization in Western Blotting Using Stain-Free Technology, Rev F Click to download
6359 Avoiding Housekeeping Protein Detection Saturation Protocol, Rev A Click to download
6361 Determining the Appropriate Film Exposure Time Protocol, Rev A Click to download
6362 Determining the Appropriate Sample Load for Western Blots Protocol, Rev A Click to download
6363 Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow Protocol, Rev A Click to download
6366 Validating the Expression Consistency of a Housekeeping Protein Protocol, Rev A Click to download
6376 General Protocol for Western Blotting, Rev B Click to download
6356 Traditional vs. V3 Western Workflow Click to download
6360 A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein Quantitation, Rev B Click to download
6390 General V3 Western Workflow Blotting Protocol, Rev A Click to download
RP0058 A Defined Methodology for Reliable Quantification of Western Blot Data Click to download
5781 Evaluation of the Criterion Stain Free Gel Imaging System for Use in Western Blotting Applications, Rev A Click to download