UNOsphere™ Rapid S Media

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Overview

Stability of UNOsphere Rapid S

Stability of UNOsphere Rapid S media. Results from an accelerated storage study (in 0.1 M NaOH) show no loss in dynamic binding capacity in an equivalent-to-5-year test. DBC, dynamic binding capacity.

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Pressure/Flow Performance of UNOsphere Rapid S

UNOsphere Rapid S media performance. Pressure/flow performance for a 20 cm diameter x 20 cm bed height column packed to 17% axial compression.

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UNOsphere Rapid S Media Dynamic Binding Capacity

Dynamic binding capacity (DBC) vs. flow velocity. A column 1.1 cm in diameter was packed to a 20 cm bed height with UNOsphere Rapid S media; 2 mg/ml human IgG in 50 mM sodium acetate was loaded until 10% breakthrough was observed.

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An example of separation. A, chromatogram of IgG purification from calf serum (green trace) and purified bovine IgG (blue trace) using the the same binding and elution conditions. B, chromatography conditions.

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UNOsphere Rapid S strong cation exchange media have been optimized to deliver speed and throughput in capture and intermediate purification steps. The support combines high binding capacity, fast mass transfer, excellent flow characteristics, and robust physical properties to make it an exceptional choice for process chromatography.

UNOsphere Rapid S support:

  • Stands up to sanitization and cleaning — The performance of UNOsphere Rapid S support is not compromised by exposure to NaOH in commonly used sanitization and clean-in-place protocols
  • Exhibits high flow at low backpressure — The macroporous structure of the UNOsphere matrix affords the use of high flow rates while maintaining very low backpressures. With typical buffers and running conditions, it is possible to achieve linear velocities in excess of 1,000 cm/hr with backpressures below 2 bar
  • Is designed for reproducibility — UNOsphere media are based on a unique polymerization process, which yields macroporous, hydrophilic beads that are ideal for biomolecule separations. Incorporation of the sulfonic acid ligand directly into the matrix during the polymerization process ensures consistent batch-to-batch and run-to-run reproducibility

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

For more information, request bulletin 5734.

Type of ion exchanger Strong cation
Functional group -SO3-
Total ionic capacity 280 ± 30 µeq/ml
Dynamic binding capacity*  
150 cm/hr 60 mg IgG/ml
1,200 cm/hr 30 mg IgG/ml
Median particle size 80 µm
Recommended linear flow rate range** 50–1,200 cm/hr
Chemical stability  
1.0 M NaOH (20°C) Up to 2,000 hr
1.0 M HCl (20°C) Up to 200 hr
pH stability range pH 1–14
Regeneration conditions 1–2 M NaCl
Storage conditions 20% Ethanol
0.1 N NaOH for 30 days
* 10% breakthrough capacity determined with 4.5 mg/ml human IgG in a 1.1 x 10 cm column.  
** UNOsphere S support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.  
UNOsphere Rapid S Media #156-0211
UNOsphere Rapid S Media

156-0211
25 ml, strong cation exchange chromatography media, 100 μm particle size

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UNOsphere Rapid S Media #156-0213
UNOsphere Rapid S Media

156-0213
100 ml, strong cation exchange chromatography media, 100 μm particle size

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UNOsphere Rapid S Media #156-0215
UNOsphere Rapid S Media

156-0215
500 ml, strong cation exchange chromatography media, 100 μm particle size

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UNOsphere Rapid S Media #156-0217
UNOsphere Rapid S Media

156-0217
10 L, strong cation exchange chromatography media, 100 μm particle size

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Foresight™ UNOsphere™ Rapid S Plates

732-4712
2 x 96-well plates, 20 μl, filter plate prepacked with UNOsphere Rapid S strong cation exchange chromatography media

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Foresight™ UNOsphere™ Rapid S RoboColumn Unit

732-4816
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with UNOsphere Rapid S strong cation exchange chromatography media

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Foresight™ UNOsphere™ Rapid S RoboColumn Unit

732-4817
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with UNOsphere Rapid S strong cation exchange chromatography media

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Foresight™ UNOsphere™ Rapid S Column

732-4731
Pkg of 1, 1 ml, column prepacked with UNOsphere Rapid S strong cation exchange chromatography media

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Foresight™ UNOsphere™ Rapid S Column

732-4751
Pkg of 1, 5 ml, column prepacked with UNOsphere Rapid S strong cation exchange chromatography media

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Number Description Options
5734 UNOsphere Rapid S Media Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5735 Purification of a Monoclonal Antibody Using a Combination of UNOsphere Rapid S and UNOsphere Q Media Ion Exchange Chromatography After Protein A Capture, Rev A Click to download [ Add to Cart (Free) ]
10010339 Instruction Manual, UNOsphere Rapid S Media, Rev A Click to download
10005454 Instruction Manual, Bio-Scale Mini UNOsphere Q & S Cartridges, 1 and 5 ml, Rev C Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download