UNOsphere™ Cation Exchange Resin

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UNOsphere Cation Exchange Resin

Overview

Specifications
  UNOsphere™ Q UNOsphere S
Type of ion exchanger Strong anion Strong cation
Functional group -N+(CH3)3 -SO3-
Total ionic capacity 120 µeq/ml 280 µeq/ml
Dynamic binding capacity*    
   150 cm/hr 180 mg BSA/ml 60 mg IgG/ml
   600 cm/hr 125 mg BSA/ml 30 mg IgG/ml
Shipping counterion Cl- Na+
Median particle size 120 µm 80 µm
Recommended linear flow rate range** 50–1,200 cm/hr 50–1,200 cm/hr
Chemical stability    
   1.0 M NaOH (20°C) Up to 10,000 hr Up to 10,000 hr
   1.0 M HCl (20°C) Up to 200 hr Up to 200 hr
Volume changes**    
   pH 4–10 <5% <5%
   0.01–1.0 M NaCl <5% <5%
pH stability 1–14 1–14
Autoclavability Yes Yes
Antimicrobial agent 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol or 0.1 M NaOH 20% ethanol or 0.1 M NaOH

* 10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere S) and 2.0 mg/ml BSA (UNOsphere Q) in a 1.1 x 20 cm column.
** UNOshpere packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.

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UNOsphere ion exchange resins are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic resin. UNOsphere, a hydrophilic polymeric support, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

UNOsphere S support is used for strong cation exchange chromatography. UNOsphere Rapid S resin builds on the properties and features of the UNOsphere family of supports with enhanced chemistries that overcome the pH shift that occurs with conductivity transitions.

Key Features

  • Efficient capture of biopharmaceutical molecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads engineered for high mechanical stability and low backpressures
  • Robust polymer designed to withstand repetitive clean-in-place cycles
  • Optimized to operate under 2 bar backpressure at velocities of 1,200 cm/hr
  • Stable to 1 M NaoH exposure to 10,000 hr
  • Availability in biopharmaceutical quantities
  • Fully supported for regulatory submissions

 

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UNOsphere S Support

1560111
25 ml, strong cation exchange chromatography media, 80 μm particle size

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UNOsphere S Support

1560113
100 ml, strong cation exchange chromatography media, 80 μm particle size

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UNOsphere S Support

156-0115
500 ml, strong cation exchange chromatography media, 80 μm particle size

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UNOsphere S Support

156-0117
10 L, strong cation exchange chromatography media, 80 μm particle size

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(Discontinued) UNOsphere™ Rapid S Media

1560211
UNOSphere Rapid S Media is no longer available. Please use UNOsphere S or Nuvia™ HR-S Media as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) UNOsphere™ Rapid S Media

1560213
UNOSphere Rapid S Media is no longer available. Please use UNOsphere S or Nuvia™ HR-S Media as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) UNOsphere™ Rapid S Media

156-0215
UNOSphere Rapid S Media is no longer available. Please use UNOsphere S or Nuvia™ HR-S Media as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) UNOsphere™ Rapid S Media

156-0217
UNOSphere Rapid S Media is no longer available. Please use UNOsphere S or Nuvia™ HR-S Media as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
10010339 Instruction Manual, UNOsphere Rapid S Media, Rev A Click to download
7026 Enhanced Purification of Similarly Charged Proteins with Self-Generated On-Column Resin pH Gradients, Rev A Click to download
2678 UNOsphere™ S Resin Technical Data, Ver D Click to download [ Add to Cart (Free) ]
2669 UNOsphere™ S Cation Exchange Resin Product Information Sheet, Ver D Click to download
5734 UNOsphere Rapid S Media Product Information Sheet, Rev B Click to download
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6867 UNOsphere™ Chromatography Resins Publications List, Ver A Click to download
2774 Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A Click to download
2780 Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A Click to download

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