UNOsphere Anion Exchange Resin

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UNOsphere Anion Exchange Resin

Overview

Specifications
  UNOsphere Q UNOsphere S
Type of ion exchanger Strong anion Strong cation
Functional group -N+(CH3)3 -SO3-
Dynamic binding capacity*    
150 cm/hr 180 mg BSA/ml 60 mg IgG/ml
600 cm/hr 125 mg BSA/ml 30 mg IgG/ml
Shipping counterion Cl- Na+
Median particle size 120 µm 80µm
Recommended linear flow rate range** 50–1,200 cm/hr 50–1,200 cm/hr
Chemical stability    
1.0 M NaOH (20°C) Up to 10,000 hr Up to 10,000 hr
1.0 M HCl (20°C) Up to 200 hr Up to 200 hr
Volume changes**    
pH 4 – 10 <5% <5%
0.01 – 1.0 M NaCl <5% <5%
pH stability 1 – 14 1 – 14
Autoclavability Yes Yes
Antimicrobial agent 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol or 0,1 M NaOH 20% ethanol or 0,1 M NaOH

* 10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere S) and 2.0 mg/ml BSA (UNOsphere Q) in a 1.1 x 20 cm column.
** UNOshpere packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.

Product Selection by Technique
Chromatography Product Selection by Technique*
 
   
Scale
Packaging Format
     
Characteristics Product Type Analytical Pilot/Preparative Process Prepacked Bottled
 
Ion Exchange Chromatography
Separation by charge UNOsphere media     UNOsphere Q, S, rapid S
General biomolecules purification Macro-Prep media     Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications Macro-Prep 25 media     Macro-Prep 25 Q, 25 S
UNO columns   UNO Q, S  
Bio-Scale Mini Cartridges     UNOsphere Q, S  
High capacity         Macro-Prep High Q, S  
Good Capture technique AG resins   AG
Chelex resins Chelex
Bio-Rex resins Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique Selectivity CHT 80 µm support     CHT, Types I and II
Alternative to protein A for antibody purification CHT 40 µm support     CHT, Types I and II
CHT 20 µm support   CHT, Types I and II
CFT 40 µm support CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA Bio-Gel HT and HTP media     Bio-Gel HT, HTP
Bio-Scale columns   CHT Type I (10 µm)  
CHT Type II (20 µm)  
Affinity Chromatography
Purification of His- and GST- tagged proteins Profinity IMAC and GST media   Bio-Scale Mini Profinity IMAC
Separation by biological interaction Affi-Gel resins     Affi-Gel Protein A**
Affi-Gel Blue**
DEAE Affi-Gel Blue**
CM Affi-Gel Blue**
High selectivity           Affi-Gel boronate, heparin**
High purity in a single step           Affi-Gel Hz, 10, 15***
Affi-Gel 102***
Excellent capture technique Profinity epoxide media     Profinity epoxide***
Affi-Prep media   Affi-Prep protein A**
Affi-Prep polymyxin**
Bio-Scale Mini cartridges   Profinity IMAC and GST  
Econo-Pac columns   Affi-Gel Blue  
DEAE Affi-Gel Blue
Affi-Gel protein A
Size Exclusion Chromatography
Separation by size Bio-Gel P media     Bio-Gel P
MW determination Bio-Beeds S-X media     Bio-Beads S-X
Applicable to all antibody purifications Bio-Sil and Bio-Silect columns     Bio-Sil, Bio-Silect  
Desalting or buffer exchange Bio-Scale Mini cartridges     Bio-Gel P-6  
High resolution Econo-Pac columns     Econo-Pac 10DG  
Hydrophobic Interaction Chromatography
Separation by hydrophobicity Macro-Prep HIC media     Macro-Prep t-butly
Macro-Prep methyl
 

** Ready to use.
*** Activated.

UNOsphere Q support
Binding and backpressure properties of UNOsphere mediaBinding and backpressure properties of UNOsphere media. Column size, 1.1 x 20 cm. UNOsphere Q media loaded with 5mg/ml BSA in 10 mM Tris, pH 8.5. Backpressure (orange); 10% breakthrough capacity (blue).
Plasmid DNA

Plasmid DNA separation using UNOsphere media.
Analysis of DNA

Analysis of fractions generated from DNA purification using UNOsphere media.
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UNOsphere Q support is used for strong anion exchange chromatography. UNOsphere ion exchange resins are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic resin. UNOsphere, a hydrophilic polymeric support, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

Key Features

  • Efficient capture of biopharmaceutical molecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads engineered for high mechanical stability and low backpressures
  • Robust polymer designed to withstand repetitive clean-in-place cycles
  • Optimized to operate under 2 bar backpressure at velocities of 1,200 cm/hr
  • Stable to 1 M NaOH exposure to 10,000 hr
  • Availability in biopharmaceutical quantities
  • Fully supported for regulatory submissions

 

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UNOsphere Q Support

1560101
25 ml, strong anion exchange chromatography media, 120 μm particle size

0 1560101 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0101_0921120649799.xml 1560101 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1560101-unosphere-q-support 1560101 UNOsphere Q Support UNOsphere Q Support 25 ml, strong anion exchange chromatography media, 120 μm particle size 25 ml, strong anion exchange chromatography media, 120 μm particle size 25 ml, strong anion exchange chromatography media, 120 μm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/156-0101_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/156-0101_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/156-0101_feature.jpg RepEx 156-0101 25 ml, strong anion exchange chromatography media, 120 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1560101, unosphere, q, support, strong, anion, exchange, iex, ion, chromatography, media, medium, resin, protein, separation, purification, 120um, 120, 20, particle, size false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560101&CartQuote=QT&quantity= false true false $99.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560101&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560101&quantity= 1560101
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UNOsphere Q Support

1560103
100 ml, strong anion exchange chromatography media, 120 μm particle size

1 1560103 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0103_0921120649749.xml 1560103 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1560103-unosphere-q-support 1560103 UNOsphere Q Support UNOsphere Q Support 100 ml, strong anion exchange chromatography media, 120 μm particle size 100 ml, strong anion exchange chromatography media, 120 μm particle size 100 ml, strong anion exchange chromatography media, 120 μm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/156-0103_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/156-0103_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/156-0103_feature.jpg RepEx 156-0103 100 ml, strong anion exchange chromatography media, 120 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1560103, unosphere, q, support, strong, anion, exchange, iex, ion, chromatography, media, medium, resin, protein, separation, purification, 120um, 120, 20, particle, size false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560103&CartQuote=QT&quantity= false true false $310.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560103&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560103&quantity= 1560103
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UNOsphere Q Support

156-0105
500 ml, strong anion exchange chromatography media, 120 μm particle size

2 1560105 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0105_0921120649822.xml 1560105 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1560105-unosphere-q-support 1560105 UNOsphere Q Support UNOsphere Q Support 500 ml, strong anion exchange chromatography media, 120 μm particle size 500 ml, strong anion exchange chromatography media, 120 μm particle size 500 ml, strong anion exchange chromatography media, 120 μm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/156-0105_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/156-0105_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/156-0105_feature.jpg RepEx 156-0105 500 ml, strong anion exchange chromatography media, 120 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1560105, unosphere, q, support, strong, anion, exchange, iex, ion, chromatography, media, medium, resin, protein, separation, purification, 120um, 120, 20, particle, size false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560105&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560105&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560105&quantity= 156-0105
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UNOsphere Q Support

156-0107
10 L, strong anion exchange chromatography media, 120 μm particle size

3 1560107 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0107_0921120649775.xml 1560107 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1560107-unosphere-q-support 1560107 UNOsphere Q Support UNOsphere Q Support 10 L, strong anion exchange chromatography media, 120 μm particle size 10 L, strong anion exchange chromatography media, 120 μm particle size 10 L, strong anion exchange chromatography media, 120 μm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/156-0107_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/156-0107_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/156-0107_feature.jpg RepEx 156-0107 10 L, strong anion exchange chromatography media, 120 μm particle size 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1560107, unosphere, q, support, strong, anion, exchange, iex, ion, chromatography, media, medium, resin, protein, separation, purification, 120um, 120, particle, size, analytical, semi-preparative, process, scale false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560107&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560107&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560107&quantity= 156-0107
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Number Description Options
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
2729 UNOsphere™ Q Support Technical Data, Rev A Click to download [ Add to Cart (Free) ]
2724 UNOsphere™ Q Anion Exchange Resin Product Information Sheet, Ver E Click to download [ Add to Cart (Free) ]
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6786 Monoclonal Antibody Purification Resin Selection Card, Ver A Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6791 Virus Purification Resin Selection Card, Ver A Click to download
6867 UNOsphere™ Chromatography Resins Publications List, Ver A Click to download
6862 Monoclonal Antibody Purification: Intermediate Purification Resins Brochure, Ver A Click to download
6886 Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A Click to download

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