Transfer Buffer Reagents

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Dithiothreitol DTT, EDTA, Gelatin, Glycine, Iodoacetamide IAA, 2 Mercapotethanol, Tributylphosphine TBP, Tricine, Tris, and Urea  – Transfer Buffer Reagents

Overview

Specifications
DTT, Cleland's Reagent
MW 154.3
A283 ≤0.04 (20 mM solution)
White crystalline appearance
Solid melting point: ≥39°C
Iodoacetamide
>98%
Melting point ≥90°C
MW 185
Formula ICH2CONH2
Tris Base (Tris[hydroxymethyl] aminomethane or Trimethamine)
≥99.8%
MW 121.14
Heavy metals (as Pb) ≤2 ppm
A280 <0.02 (10% aqueous solution)
White crystalline appearance
EDTA (Disodium Ethylenediaminetetraacetate Dihydrate)
≥99%
MW 372.26
Heavy metals (as Pb) ≤10 ppm
Fe ≤0.01%
Insolubles ≤0.005%
pH of a 5% solution = 4.0–6.0
2-Mercaptoethanol (β-Mercaptoethanol, BME) ≥98%
MW 78.11
14.2 M
Water ≤0.5%
Thiodiglycol ≤0.5%
bis-(2-hydroxyethyl)disulfide ≤0.5%
Urea (Carbamide)
MW 60.06
A280 ≤0.05 (5.0 M solution)
Conductivity ≤15 μS
Heavy metals (as Pb) ≤5 ppm
Fe ≤1 ppm
Cyanate ≤0.002%
Biuret test negative
Turbidity ≤2.0 NTU
(last 2 tests in 5.0 M solution)
Glycine (Aminoacetic Acid)
MW 75.07
A280 <0.15 (1.0 M solution)
Heavy metals (as Pb) ≤20 ppm
NH4 ≤75 ppm
Turbidity ≤2.0 NTU
Insolubles ≤0.005%
(last 3 tests in 10% aqueous solution)
Tributylphosphine
>98%
OD ≥0.5 or ≥177 mM
MW 202.3
Formula C12H27P
Gelatin (Type A From Porcine Skin)
75–100 bloom
Tricine (N-Tris[hydroxymethyl] methylglycine)
MW 179.17
A280 ≤0.02 (0.1 M solution)

Note: All electrophoresis reagents meet specifications and have a 1 year guaranteed shelf life. Bulk and custom packaging are available for all reagents.

Bio-Rad offers a complete line of reagents for preparation of transfer buffers:

  • Dithiothreitol (DTT, Cleland's Reagent)
  • Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA)
  • Gelatin (type A from porcine skin)
  • Glycine (aminoacetic acid)
  • Iodoacetamide
  • 2-Mercaptoethanol (β-mercaptoethanol, BME)
  • Tributylphosphine (TBP)
  • Tricine (N-[Tris(hydroxymethyl)methyl]glycine)
  • Tris base (Tris[hydroxymethyl]aminomethane or trimethamine)
  • Urea (carbamide)
Dithiothreitol (DTT)

161-0610
1 g, dithiothreitol (DTT) reducing agent

List Price:   $26.00
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Dithiothreitol (DTT)

161-0611
5 g, dithiothreitol (DTT) reducing agent

List Price:   $76.00
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EDTA

161-0729
500 g, ≥99% pure EDTA (disodium ethylenediaminetetraacetate dihydrate) powder

List Price:   $67.00
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Gelatin

170-6537
200 g, enzyme immunoassay grade gelatin

List Price:   $53.00
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Glycine

161-0717
250 g, glycine (aminoacetic acid) powder

List Price:   $23.00
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Glycine

161-0718
1 kg, glycine (aminoacetic acid) powder

List Price:   $50.00
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Glycine

161-0724
2 kg, glycine (aminoacetic acid) powder

List Price:   $99.00
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Iodoacetamide

163-2109
30 g, alkylating agent for use in protein sample preparation applications

List Price:   $133.00
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Hazardous  
2-Mercaptoethanol

161-0710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

List Price:   $22.00
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Hazardous  
Tributylphosphine (TBP)

163-2101
0.6 ml, 200 mM, reducing agent for use in protein sample preparation applications

List Price:   $41.00
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Tricine

161-0713
500 g, Tricine (N-Tris[hydroxymethyl]methylglycine) powder

List Price:   $189.00
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Tris

161-0716
500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

List Price:   $32.00
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Tris

161-0719
1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

List Price:   $79.00
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Urea

161-0730
250 g, urea (carbamide)

List Price:   $26.00
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Urea

161-0731
1 kg, urea (carbamide)

List Price:   $71.00
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Number Description Options
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]