T100™ Thermal Cycler



T100™ Thermal Cycler — User Reviews

Hear researchers from around the world describe their unique experiences using the T100 thermal cycler. The T100 thermal cycler’s intuitive touch screen makes running PCR easier than ever before. The performance, features, and ease of use are efficiently streamlined into a compact footprint that fits in any laboratory. With the T100 thermal cycler you can:

  • Save time programming with the intuitive touch screen
  • Get superior results by using the thermal gradient to optimize your assays in a single run
  • Save valuable benchspace with the compact design
  • Be confident in your results with the reliability you expect from Bio-Rad
T100™ Thermal Cycler System Tour
Intuitive Programming

The 5.7'' high-resolution touch screen makes it easy to create a protocol, start a run, or manage files, so you spend less time programming.


  • Save time with streamlined graphical programming
  • Stay organized with personalized folders
  • Effortlessly transfer protocols among instruments using a USB flash drive
  • Get started quicker with the built-in library of standard protocols for long PCR, fast PCR, reverse transcription PCR, and more
Home screen.   New Protocol screen.


Thermal Gradient 

Optimize Reactions in a Single Run

Thermal gradient technology allows you to quickly optimize your PCR assays in a single run by simultaneously testing eight different temperatures across a range of up to 25°C. The optimal annealing temperature for a PCR assay maximizes yield while preventing the formation of nonspecific products. The dynamic ramping technology adjusts the cycler ramp rate, ensuring that the incubation times across the eight rows during a gradient step are identical.

Optimization of an assay results in better yields and specificity. Results show that assays A and B can be run at an annealing temperature of 57.9°C on the same plate. Higher temperatures result in a reduced yield in assay A while lower temperatures result in nonspecific products in assay B. M, markers.

Thermal Cycler Selection Chart
C1000 Touch™


Peltier-effect technology
Upgrade to real-time PCR
Interchangeable sample blocks
Number of wells 96, 96 deep, dual 48, or 384 96, 96 deep, dual 48, or 384 96
Gradient capability
Graphical user interface
Fast PCR protocol templates
Choice of temperature control mode
Power failure restore
Programmable ramp rates
Networking capability
Remote command set for automation
Reports on cycler use and performance
Heated lid Adjustable Adjustable Fixed
Full-skirted plate compatibility

PCR Plastics Compatibility Chart

Compatibility of PCR Plastic Consumables with Bio-Rad Thermal Cyclers


Thermal Cyclers
MJ Mini™ Gradient Thermal Cycler

T100™ Thermal Cycler

DNA Engine® Thermal Cycler Family

C1000 Touch™/S1000™ Thermal Cyclers
0.5 ml individual tubes   Deep well
module only
0.2 ml individual tubes
High-profile tube strips
Low-profile tube strips  
48- and 96-Well Plates
Hard-Shell® low-profile 96-well semi-skirted PCR plates      
Hard-Shell high-profile 96-well semi-skirted PCR plates  
Hard-Shell low-profile 96-well skirted PCR plates    
Multiplate™ high-profile unskirted PCR plates
Multiplate low-profile unskirted PCR plates  
iQ™ 96-well semi-skirted PCR plates  
384-Well Plates
Hard-Shell 384-well skirted PCR plates    
Domed cap strips
Optical flat cap strips
Adhesive Seals
Microseal® 'A' film  
Microseal 'B' adhesive seals  
Microseal ꞌCꞌ optical seals  
Microseal 'F' foil  
Heat Sealer and Heat Sealing Films and Foils
PX1™ PCR plate sealer  
Optically clear heat seal  
Pierceable foil heat seal  
Peelable foil heat seal  

The T100 thermal cycler is a small thermal cycler offering a comprehensive set of convenient features in a small footprint. This compact thermal cycler features an intuitive touch-screen user interface to make running PCR easy. Thermal gradient technology allows you to quickly optimize your reaction in a single run. With its robust design, the T100 system is a reliable personal thermal cycler that delivers exceptional performance for years — test drive the T100 thermal cycler today

Features and Benefits of the T100 Thermal Cycler

  • Intuitive touch screeneasy programming using a large color touch screen and intuitive interface
  • Consistent results — robust design ensures effective and consistent performance run to run
  • Easy optimization — this gradient thermal cycler allows fast PCR optimization using a unique thermal gradient
  • Easy protocol management — protocols can be organized using personalized folders or a USB flash drive
  • Small space-saving footprint — the T100 is a compact thermal cycler that fits in any laboratory

Watch this video highlighting how researchers around the world use the T100 thermal cycler.

Applications and Uses for the T100 Thermal Cycler

More Information

Download the latest T100 firmware upgrade.

Find the perfect thermal cycler — compare the complete line of Bio-Rad PCR systems in the Thermal Cycler Selection Chart.

 View the PCR Plastics Compatibility Chart to find the right consumables for your instrument.

Thermal Cycler  
Sample capacity 96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate
Maximum ramp rate, °C/sec 4
Average ramp rate, °C/sec 2.5
Temperature range 4–100°C
Temperature accuracy ±0.5°C of programmed target
Temperature uniformity ±0.5°C well-to-well within 30 sec of arrival at target temperature
Input power 100–150 VAC, 50–60 Hz; 220–240 VAC, 50–60 Hz; 700 W maximum
Display 5.7'' VGA color touch screen
Port 1 USB A
Memory 500 typical programs; unlimited with USB flash drive expansion
Dimensions (W x D x H) 26 x 47 x 23 cm (10 x 18 x 9'')
Weight 9 kg (20 lb)
Gradient range 30–100°C
Temperature differential range 1–25°C

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T100™ Thermal Cycler

Thermal cycler system, includes 96-well thermal cycler, power cord, tube support ring

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Touch-Screen Protector

Pkg of 2, optional clear plastic cover for the T100™ thermal cycler that shields the touch-screen surface from dust, reagents, and scratches

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Tube Support Ring

Pkg of 2, extra tube support ring for use with individual tubes in the T100™ thermal cycler

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Number Description Options
10020524 T100 Thermal Cycler Quick Guide, Rev B Click to download [ Add to Cart (Free) ]
10021426 Amplification Plastics and Reagents Selection Guide for the T100™ Thermal Cycler, Rev B Click to download
5277 Bio-Rad Thermal Cyclers Brochure, Rev G Click to download [ Add to Cart (Free) ]
10020523 Instruction Manual, T100 Thermal Cycler, Rev B Click to download
6777 Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR, Ver A Click to download