Supported Nitrocellulose Membrane, 0.45 µm

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Overview

Available Formats

Blotting membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Supported Nitrocellulose Membranes

Ideal for:

  • Protein blotting
  • DNA/RNA blotting
  • Dot/slot blot screening

 

Bio-Rad's 0.45 µm pore-size supported nitrocellulose membrane is dense 100% nitrocellulose cast on both sides of an inert polyester support. It is easy to handle, wets readily, and does not have the brittleness often associated with unsupported nitrocellulose.

This supported 0.45 µm nitrocellulose has an excellent signal-to-noise ratio and is recommended for analytical blotting, including most protein, DNA, and RNA transfers. For low molecular weight proteins and nucleic acids, use our 0.2 µm supported nitrocellulose membrane.

Features and Benefits

  • Compatible with all standard immunoblotting and nucleic acid detection methods
  • Provides superior binding capacity without background interference
  • Pore size produces excellent signal-to-noise blotting results
  • Available in in packs of precut membrane or an economical roll

Applications

  • Transfer of protein from SDS-PAGE gels to membrane in preparation for western blotting
  • Transfer of a wide range of protein molecular weights and nucleic acids >500 bp
  • Transfer of nucleic acids in preparation for Northern or Southern blotting
  • Immunodetection of target proteins and probe hybridization of nucleic acids

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

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Supported Nitrocellulose Membrane

1620094
Pkg of 1 roll, 0.45 μm, 30 cm x 3 m, bulk blotting membrane for proteins and nucleic acids

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$315.00
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(Discontinued) Supported Nitrocellulose Membranes

1620093
Pkg of 10, 0.45 μm, 20 x 20 cm, precut blotting membrane for proteins and nucleic acids

This product is no longer available. Please contact technical support for more information on replacement product.
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Supported Nitrocellulose Membranes

1620090
Pkg of 10, 0.45 μm, 7 x 8.4 cm, precut blotting membrane for proteins and nucleic acids

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$61.00
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Supported Nitrocellulose Membranes

1620070
Pkg of 10, 0.45 μm, 8.5 x 13.5 cm, precut blotting membrane for proteins and nucleic acids

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$94.00
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(Discontinued) Supported Nitrocellulose Membranes

1620254
Pkg of 10, 0.45 μm, 26.5 x 28 cm, precut blotting membrane for proteins and nucleic acids

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]