StarBright™ Blue 700 Fluorescent Secondary Antibodies

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StarBright Fluorescent Secondary Antibodies

Overview

Triplex Blot Example
Triplex blot image
Simultaneous detection of multiple proteins (ATG7, AKR1C2, and tubulin) on a single blot using fluorescent antibodies.
Target Protein Color 1º Antibody 2º Antibody Exposure Time (seconds)
ATG7 Blue ATG7 Rabbit Anti-Human
(78 kDa)
StarBright™ Blue 700 Goat Anti-Rabbit 6.9
AKR1C2 Red AKR1C2 Mouse Anti-Human (36 kDa) Goat Anti-Mouse IgG DyLight 800 300.0
Tubulin Green hFAB™ Rhodamine Anti-Tubulin
(50 kDa)
141.9


Detection of Post-Translational Modifications
Composite blot image
Composite blot image of triplex western blotting showing post-translational phosphorylation of a target protein.
Target Protein Color 1° Antibody 2° Antibody
Phospho-ERK 1/2
Red StarBright™ Blue 700 Goat Anti-Rabbit
Total ERK 1/2
Green Goat Anti-Mouse IgG DyLight 800
GAPDH Blue hFAB™ Rhodamine Anti-GAPDH
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StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.

StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

Schematic representation of a StarBright Blue 700 Label conjugated to an antibody. The light-absorbing monomer is excited at 470 nm and, via an efficient intramolecular transfer process, the energy is transmitted to a light-emitting monomer, which emits at 700 nm. An antibody is conjugated to linker monomers.

Features and Benefits

  • Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot
  • Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
  • High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm)
  • Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

Applications and Uses

StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores as described in the table below. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.

Note: StarBright Antibodies are not suitable for stripping and reprobing.

Recommended Fluorophore Combinations
Targets Primary Target
(Fluorophore A)
Second Target
(Fluorophore B)
Third Target
(Fluorophore C)
1 StarBright Blue 700
2 StarBright Blue 700 hFAB Rhodamine HKP* Antibody (α-actin/tubulin/GAPDH)
3 StarBright Blue 700 DyLight 800 hFAB Rhodamine HKP Antibody (α-actin/tubulin/GAPDH)

* Stain-free gels can be used for total protein normalization in place of or in addition to housekeeping proteins (HKP).

Related Products

Related Categories

Supporting Documents

  • hFAB Rhodamine Housekeeping Antibodies Instruction Manual
  • Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies Selection Guide
  • StarBright Blue 700 Secondary Antibodies Flier

Online Resources

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StarBright™ Blue 700 Goat Anti-Mouse IgG, 80 µl

12004159
Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 80 µl

12004159 /templatedata/internet/product_SKU/data/LSR/Antibodies/12004159-_1486421843.xml 12004159 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jav5wozsjav5wozt" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jav5wozsjav5wozt"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004159-_1486421843.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzi" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzi"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004159-_1486421843.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzo" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzo"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004159-_1486421843.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Node> </Deployments> 02/06/17 02:51 PM en_US jav5worw 2017-11-14 07:07:41 true true internet/product_SKU LSR N en 02/06/27 02:56 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:11:16 12004159-starbright-blue-700-goat-anti-mouse-igg-80-ul 12004159 Goat Anti-Mouse IgG SB Blue 700 80ul Kit StarBright&trade; Blue 700 Goat Anti-Mouse IgG, 80 &micro;l Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 80 &micro;l /webroot/web/images/lsr/products/antibody/sku_thumb/global/starbright-mouse-80ul-12004159-tn.jpg /webroot/web/images/lsr/products/antibody/sku_view/global/starbright-mouse-80ul-12004159-view.jpg Goat Anti-Mouse IgG StarBright&trade; Blue 700, 80 &micro;l preview Karen Moss StarBright™ Blue 700 Goat Anti-Mouse IgG, 80 µl Pkg of 1, 80 µl tube of lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 fluorophore western blot, immunodetection, fluorophore, 470 nm, 700 nm, 470/700, blue fluorescent, goat, anti-mouse, igg 02/06/17 02:51 PM 02/06/27 02:56 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Antibodies N 0 <p>Use StarBright Blue 700 Goat Anti-Mouse IgG for ultra-sensitive detection of proteins in fluorescence western blotting. This secondary antibody is ideal for use in multiplex blotting, eliminating the need for stripping and reprobing, or cutting blots. Goat Anti-Mouse IgG Starbright Blue 700 is optimized for use with the ChemiDoc&trade; MP Imaging System, permitting detection of up to three proteins in a single blot.</p> <p>The StarBright Fluorophore is composed of multiple monomers that each absorb and emit light, giving a higher quantum yield than other fluorophores and creating a very strong signal. Additionally, the large separation between Ex<sub>max</sub> and Em<sub>max</sub> (470/700 nm) results in minimal spectral overlap between emission and excitation wavelengths. <br /></p> <p><img src="/webroot/web/images/lsr/products/antibody/sku_view/global/schematic-representation-of-starbright2.jpg" alt="" width="600" height="200" /></p> <p class="caption"><strong>Schematic representation of a StarBright Label conjugated to an antibody. </strong>The light-absorbing monomer is excited at 470 nm, and via an efficient intra-molecular energy transfer process, the energy is transmitted to a light-emitting monomer which emits at 700 nm. The linker monomer is used for antibody conjugation.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li><strong>Easy multiplexing &mdash; </strong>can be used with IRDye 800 Antibodies or other common fluorophores to detect multiple proteins on the same blot</li> <li> <strong>Shorter exposure time &mdash; </strong>the exceptional brightness of the StarBright Fluorophore leads to exposure times orders of magnitude lower than traditional fluorescent dyes: seconds vs. minutes </li> <li> <strong>High signal-to-noise ratio &mdash; </strong>emits in the infrared range of the spectrum, where there is minimal background (excitation/emission: 470/700 nm)</li> <li> <strong>Low nonspecific binding &mdash; </strong>conjugated to highly cross-adsorbed antibodies</li> </ul> <h2 class="descHeader">Product Options</h2> <p>StarBright Blue 700 Secondary Antibodies can be used for <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/triplex-blot-image-desc.html&quot;);">multiplex fluorescent western blotting</a> with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/detection-post-translational-modifications-desc.html&quot;);">detect post-translational modifications</a> like phosphorylation of a target protein.</p> <p><strong>Note:</strong> StarBright Antibodies are not suitable for stripping and reprobing.<br /></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"> <p><strong>Products</strong><strong> </strong></p> </td> <td align="center" valign="top"> <p><strong>Catalog Number</strong><strong> </strong></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p>12004159</p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004158">12004158</a></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004157">12004157</a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004162">12004162</a></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004161">12004161</a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004160">12004160</a></p> </td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/hfab-rhodamine-housekeeping-protein-fluorescent-primary-antibodies?ID=OKNWY3KG4">hFAB&trade; Rhodamine Housekeeping Protein Fluorescent Primary Antibodies</a></li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Primary Antibodies</a></li> <li><a href="/en-us/product/prestained-protein-standards">Precision Plus Protein&trade; Prestained Protein Standards</a></li> <li><a href="/en-us/product/chemidoc-mp-imaging-system">ChemiDoc MP Imaging System</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-ca/category/fluorescent-western-blotting-antibodies">Fluorescent Western Blotting Antibodies</a></li> <li><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade; Precast Gels</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>StarBright Blue 700 Fluorescent Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000066837.pdf" target="_blank">Instruction Manual</a></li> <li>Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000068973.pdf" target="_blank">Selection Guide</a></li> <li> StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/Bulletin_6911.pdf" target="_blank">Flier</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="http://www.bioradiations.com/the-fluorescent-way-why-start-fluorescent-western-blotting-now" target="_blank">The Fluorescent Way: Why Start Fluorescent Western Blotting Now?</a></li> <li><a href="http://www.bioradiations.com/?s=Helpful+Tips+for+Optimizing+Your+Protocol" target="_blank">Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol</a></li> </ul> /webroot/web/html/lsr/products/antibody/sku_view/description/global goat-anti-mouse-igg-starbright-blue-700-80-desc.html /webroot/web/html/lsr/products/antibody/sku_view/description/global/goat-anti-mouse-igg-starbright-blue-700-80-desc.html Shelf life 2149 1 year at &ndash;20&deg;C lyophilized; 6 months at 4&deg;C after resuspension 1 0 Recommended dilution 3036 1:2,500&ndash;1:5,000 1 0 Cross-adsorbed species 3038 Bovine, goat, human, rabbit, and rat 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004159&CartQuote=QT&quantity= false true false $52.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004159&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004159&quantity= 12004159
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StarBright™ Blue 700 Goat Anti-Mouse IgG, 400 µl

12004158
Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 400 µl

12004158 /templatedata/internet/product_SKU/data/LSR/Antibodies/12004158-_1486421410.xml 12004158 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jav5wozsjav5wozt" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jav5wozsjav5wozt"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004158-_1486421410.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzi" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzi"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004158-_1486421410.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzo" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzo"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004158-_1486421410.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Node> </Deployments> 02/06/17 01:57 PM en_US jav5wosw 2017-11-14 07:07:41 true true internet/product_SKU LSR N en 02/06/27 02:49 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:11:16 12004158-starbright-blue-700-goat-anti-mouse-igg-400-ul 12004158 Goat Anti-MouseIgG SB Blue 700 400ul Kit StarBright&trade; Blue 700 Goat Anti-Mouse IgG, 400 &micro;l Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 400 &micro;l /webroot/web/images/lsr/products/antibody/sku_thumb/global/starbright-mouse-400ul-12004158-tn.jpg /webroot/web/images/lsr/products/antibody/sku_view/global/starbright-mouse-400ul-12004158-view.jpg StarBright&trade; Blue 700 Goat Anti-Mouse IgG, 400 &micro;l preview Karen Moss StarBright™ Blue 700 Goat Anti-Mouse IgG, 400 µl Pkg of 1, 400 µl tube of lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 Fluorophore 12004158, starbright, star bright, fluorescent, secondary antibody, fluorescent antibody, antibody conjugate, western blotting, western blot, immunodetection, fluorophore, 470 nm, 700 nm, 470/700, blue fluorescent, goat, anti-mouse, igg 02/06/17 01:57 PM 02/06/27 02:49 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Antibodies N 0 <p>Use StarBright Blue 700 Goat Anti-Mouse IgG for ultra-sensitive detection of proteins in fluorescence western blotting. This secondary antibody is ideal for use in multiplex blotting, eliminating the need for stripping and reprobing, or cutting blots. StarBright Blue 700 Goat Anti-Mouse IgG is optimized for use with the ChemiDoc&trade; MP Imaging System, permitting detection of up to three proteins in a single blot.</p> <p>The StarBright Fluorophore is composed of multiple monomers that each absorb and emit light, giving a higher quantum yield than other fluorophores and creating a very strong signal. Additionally, the large separation between Ex<sub>max</sub> and Em<sub>max</sub> (470/700 nm) results in minimal spectral overlap between emission and excitation wavelengths.<br /></p> <p><img src="/webroot/web/images/lsr/products/antibody/sku_view/global/schematic-representation-of-starbright2.jpg" alt="" width="600" height="200&quot;" /></p> <p class="caption"><strong>Schematic representation of a StarBright Label conjugated to an antibody.&nbsp;</strong> The light-absorbing monomer is excited at 470 nm and, via an efficient intramolecular transfer process, the energy is transmitted to a light-emitting monomer, which emits at 700 nm. An antibody is conjugated to linker monomers.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li><strong>Easy multiplexing &mdash; </strong>can be used with IRDye 800 Antibodies or other common fluorophores to detect multiple proteins on the same blot</li> <li> <strong>Shorter exposure time &mdash; </strong>the exceptional brightness of the StarBright Fluorophore leads to exposure times orders of magnitude lower than traditional fluorescent dyes: seconds vs. minutes </li> <li> <strong>High signal-to-noise ratio &mdash; </strong>emits in the infrared range of the spectrum, where there is minimal background (excitation/emission: 470/700 nm)</li> <li> <strong>Low nonspecific binding &mdash; </strong>conjugated to highly cross-adsorbed antibodies</li> </ul> <h2 class="descHeader">Product Options</h2> <p>StarBright Blue 700 Secondary Antibodies can be used for <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/triplex-blot-image-desc.html&quot;);">multiplex fluorescent western blotting</a> with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/detection-post-translational-modifications-desc.html&quot;);">detect post-translational modifications</a> like phosphorylation of a target protein.</p> <p><strong>Note:</strong> StarBright Antibodies are not suitable for stripping and reprobing.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"> <p><strong>Products</strong><strong> </strong></p> </td> <td align="center" valign="top"> <p><strong>Catalog Number</strong><strong> </strong></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004159">12004159 </a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p>12004158</p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004157">12004157</a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004162">12004162</a></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004161">12004161</a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004160">12004160</a></p> </td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/hfab-rhodamine-housekeeping-protein-fluorescent-primary-antibodies?ID=OKNWY3KG4">hFAB&trade; Rhodamine Housekeeping Protein Fluorescent Primary Antibodies</a></li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Primary Antibodies</a></li> <li><a href="/en-us/product/prestained-protein-standards">Precision Plus Protein&trade; Prestained Protein Standards</a></li> <li><a href="/en-us/product/chemidoc-mp-imaging-system">ChemiDoc MP Imaging System</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-ca/category/fluorescent-western-blotting-antibodies">Fluorescent Western Blotting Antibodies</a></li> <li><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade; Precast Gels</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>StarBright Blue 700 Fluorescent Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000066837.pdf" target="_blank">Instruction Manual</a><a href="/webroot/web/pdf/lsr/literature/10000078050.pdf" target="_blank"></a></li> <li>Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000068973.pdf" target="_blank">Selection Guide</a></li> <li>StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/Bulletin_6911.pdf" target="_blank">Flier</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="http://www.bioradiations.com/the-fluorescent-way-why-start-fluorescent-western-blotting-now" target="_blank">The Fluorescent Way: Why Start Fluorescent Western Blotting Now?</a></li> <li><a href="http://www.bioradiations.com/?s=Helpful+Tips+for+Optimizing+Your+Protocol" target="_blank">Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol</a></li> </ul> /webroot/web/html/lsr/products/antibody/sku_view/description/global starbright-blue 700-goat-anti-mouse-igg-400-12004158-desc.html /webroot/web/html/lsr/products/antibody/sku_view/description/global/starbright-blue 700-goat-anti-mouse-igg-400-12004158-desc.html Shelf life 2149 1 year at &ndash;20&deg;C lyophilized; 6 months at 4&deg;C after reconstitution 1 0 Recommended dilution 3036 1:2,500&ndash;1:5,000 1 0 Cross-adsorbed species 3038 Bovine, goat, human, rabbit, and rat 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004158&CartQuote=QT&quantity= false true false $103.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004158&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004158&quantity= 12004158
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StarBright™ Blue 700 Goat Anti-Mouse IgG, 5 x 400 µl

12004157
Pkg of 5, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 400 µl

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StarBright™ Blue 700 Goat Anti-Rabbit IgG, 80 µl

12004162
Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 80 µl

12004162 /templatedata/internet/product_SKU/data/LSR/Antibodies/12004162_1486422383.xml 12004162 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jav5wozsjav5wozt" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jav5wozsjav5wozt"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004162_1486422383.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzi" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzi"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004162_1486422383.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzo" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzo"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004162_1486422383.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Node> </Deployments> 02/06/17 03:03 PM en_US jav5worx 2017-11-14 07:07:41 true true internet/product_SKU LSR N en 02/06/27 03:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:11:16 12004162-starbright-blue-700-goat-anti-rabbit-igg-80-ul 12004162 Goat anti-Rabbit IgG StarBright Blue 700 40 microl StarBright&trade; Blue 700 Goat Anti-Rabbit IgG, 80 &micro;l Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 80 &micro;l /webroot/web/images/lsr/products/antibody/sku_thumb/global/starbright-rabbit-80ul-12004162-tn.jpg /webroot/web/images/lsr/products/antibody/sku_view/global/starbright-rabbit-80ul-12004162-view.jpg StarBright™ Blue 700 Goat Anti-Rabbit IgG, 80 µl preview Karen Moss StarBright™ Blue 700 Goat Anti-Rabbit IgG, 80 µl Pkg of 1, 80 µl tube of lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 fluorophore 12004162, starbright, star bright, fluorescent, secondary antibody, fluorescent antibody, antibody conjugate, western blotting, western blot, immunodetection, fluorophore, 470 nm, 700 nm, 470/700, blue fluorescent, goat, anti-rabbit, igg 02/06/17 03:03 PM 02/06/27 03:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Antibodies N 0 <p>Use StarBright Blue 700 Goat Anti-Rabbit IgG for ultra-sensitive detection of proteins in fluorescence western blotting. This secondary antibody is ideal for use in multiplex blotting, eliminating the need for stripping and reprobing, or cutting blots. StarBright Blue 700 Goat Anti-Rabbit IgG is optimized for use with the ChemiDoc&trade; MP Imaging System, permitting detection of up to three proteins in a single blot.</p> <p>The StarBright Fluorophore is composed of multiple monomers that each absorb and emit light, giving a higher quantum yield than other fluorophores and creating a very strong signal. Additionally, the large separation between Ex<sub>max</sub> and Em<sub>max</sub> (470/700 nm) results in minimal spectral overlap between emission and excitation wavelengths. <br /></p> <p><img src="/webroot/web/images/lsr/products/antibody/sku_view/global/schematic-representation-of-starbright2.jpg" alt="" width="600" height="200" /></p> <p class="caption"><strong>Schematic representation of a StarBright Label conjugated to an antibody. </strong>The light-absorbing monomer is excited at 470 nm and, via an efficient intra-molecular energy transfer process, the energy is transmitted to a light-emitting monomer which emits at 700 nm. The linker monomer is used for antibody conjugation.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li><strong>Easy multiplexing &mdash; </strong>can be used with IRDye 800 Antibodies or other common fluorophores to detect multiple proteins on the same blot</li> <li> <strong>Shorter exposure time &mdash; </strong>the exceptional brightness of the StarBright Fluorophore leads to exposure times orders of magnitude lower than traditional fluorescent dyes: seconds vs. minutes</li> <li> <strong>High signal-to-noise ratio &mdash; </strong>emits in the infrared range of the spectrum, where there is minimal background (excitation/emission: 470/700 nm)</li> <li> <strong>Low nonspecific binding &mdash; </strong>conjugated to highly cross-adsorbed antibodies</li> </ul> <h2 class="descHeader">Product Options</h2> <p>StarBright Blue 700 Secondary Antibodies can be used for <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/triplex-blot-image-desc.html&quot;);">multiplex fluorescent western blotting</a> with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/detection-post-translational-modifications-desc.html&quot;);">detect post-translational modifications</a> like phosphorylation of a target protein.</p> <p><strong>Note:</strong> StarBright Antibodies are not suitable for stripping and reprobing.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"> <p><strong>Products</strong><strong> </strong></p> </td> <td align="center" valign="top"> <p><strong>Catalog Number</strong><strong> </strong></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004159">12004159 </a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004158">12004158</a></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004157">12004157</a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p>12004162</p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004161">12004161</a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004160">12004160</a></p> </td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/hfab-rhodamine-housekeeping-protein-fluorescent-primary-antibodies?ID=OKNWY3KG4">hFAB&trade; Rhodamine Housekeeping Protein Fluorescent Primary Antibodies</a></li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Primary Antibodies</a></li> <li><a href="/en-us/product/prestained-protein-standards">Precision Plus Protein&trade; Prestained Protein Standards</a></li> <li><a href="/en-us/product/chemidoc-mp-imaging-system">ChemiDoc MP Imaging System</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-ca/category/fluorescent-western-blotting-antibodies">Fluorescent Western Blotting Antibodies</a></li> <li><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade; Precast Gels</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>StarBright Blue 700 Fluorescent Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000066837.pdf" target="_blank">Instruction Manual</a><a href="/webroot/web/pdf/lsr/literature/10000078050.pdf" target="_blank"></a></li> <li>Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000068973.pdf" target="_blank">Selection Guide</a></li> <li>StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/Bulletin_6911.pdf" target="_blank">Flier</a><a href="/webroot/web/pdf/lsr/literature/Bulletin_6911.pdf" target="_blank"></a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="http://www.bioradiations.com/the-fluorescent-way-why-start-fluorescent-western-blotting-now" target="_blank">The Fluorescent Way: Why Start Fluorescent Western Blotting Now?</a></li> <li><a href="http://www.bioradiations.com/?s=Helpful+Tips+for+Optimizing+Your+Protocol" target="_blank">Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol</a></li> </ul> /webroot/web/html/lsr/products/antibody/sku_view/description/global 12004162.html /webroot/web/html/lsr/products/antibody/sku_view/description/global/12004162.html Shelf life 2149 1 year at &ndash;20&deg;C lyophilized; 6 months at 4&deg;C after resuspension 1 0 Recommended dilution 3036 1:2,500&ndash;1:5,000 1 0 Cross-adsorbed species 3038 Bovine, goat, human, mouse, and rat 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004162&CartQuote=QT&quantity= false true false $52.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004162&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004162&quantity= 12004162
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StarBright™ Blue 700 Goat Anti-Rabbit IgG, 400 µl

12004161
Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 400 µl

12004161 /templatedata/internet/product_SKU/data/LSR/Antibodies/12004161-_1486422827.xml 12004161 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jav5wozsjav5wozt" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jav5wozsjav5wozt"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004161-_1486422827.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="6" DayOfWeek="3" Year="2017" Hour="22" Minute="5" Second="10" ZoneOffsetMillis="0" Time="1512597910184">Wed Dec 06 22:05:10 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzi" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzi"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004161-_1486422827.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="20" Second="57" ZoneOffsetMillis="0" Time="1512609657194">Thu Dec 07 01:20:57 UTC 2017</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.javqbdzhjavqbdzo" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="javqbdzhjavqbdzo"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Antibodies/12004161-_1486422827.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="7" DayOfWeek="4" Year="2017" Hour="1" Minute="24" Second="13" ZoneOffsetMillis="0" Time="1512609853699">Thu Dec 07 01:24:13 UTC 2017</LastExecutionTime> </Node> </Deployments> 02/06/17 03:07 PM en_US jav5wosf 2017-11-14 07:07:41 true true internet/product_SKU LSR N en 02/06/27 03:13 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:11:16 12004161-starbright-blue-700-goat-anti-rabbit-igg-400-ul 12004161 Goat Anti-RabbitIgG SB Blue700 400ul Kit StarBright&trade; Blue 700 Goat Anti-Rabbit IgG, 400 &micro;l Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 400 &micro;l /webroot/web/images/lsr/products/antibody/sku_thumb/global/starbright-rabbit-400ul-12004161-tn.jpg /webroot/web/images/lsr/products/antibody/sku_view/global/starbright-rabbit-400ul-12004161-view.jpg StarBright™ Blue 700 Goat Anti-Rabbit IgG, 400 µl preview Karen Moss StarBright™ Blue 700 Goat Anti-Rabbit IgG, 400 µl Pkg of 1, 400 µl tube of lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 fluorophore 12004161, starbright, star bright, fluorescent, secondary antibody, fluorescent antibody, antibody conjugate, western blotting, western blot, immunodetection, fluorophore, 470 nm, 700 nm, 470/700, blue fluorescent, goat, anti-rabbit, igg 02/06/17 03:07 PM 02/06/27 03:13 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Antibodies N 0 <p>Use StarBright Blue 700 Goat Anti-Rabbit IgG for ultra-sensitive detection of proteins in fluorescence western blotting. This secondary antibody is ideal for use in multiplex blotting, eliminating the need for stripping and reprobing, or cutting blots. StarBright Blue 700 Goat Anti-Rabbit IgG is optimized for use with the ChemiDoc&trade; MP Imaging System, permitting detection of up to three proteins in a single blot.</p> <p>The StarBright Fluorophore is composed of multiple monomers that each absorb and emit light, giving a higher quantum yield than other fluorophores and creating a very strong signal. Additionally, the large separation between Ex<sub>max</sub> and Em<sub>max</sub> (470/700 nm) results in minimal spectral overlap between emission and excitation wavelengths.</p> <p><img src="/webroot/web/images/lsr/products/antibody/sku_view/global/schematic-representation-of-starbright2.jpg" alt="" width="600" height="200" /></p> <p class="caption"><strong>Schematic representation of a StarBright Label conjugated to an antibody. </strong>The light-absorbing monomer is excited at 470 nm and, via an efficient intra-molecular energy transfer process, the energy is transmitted to a light-emitting monomer which emits at 700 nm. The linker monomer is used for antibody conjugation.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li><strong>Easy multiplexing &mdash; </strong>can be used with IRDye 800 Antibodies or other common fluorophores to detect multiple proteins on the same blot</li> <li><strong>Shorter exposure time &mdash;</strong> the exceptional brightness of the StarBright Fluorophore leads to exposure times orders of magnitude lower than traditional fluorescent dyes: seconds vs. minutes</li> <li> <strong>High signal-to-noise ratio &mdash; </strong>emits in the infrared range of the spectrum, where there is minimal background (excitation/emission: 470/700 nm)</li> <li> <strong>Low nonspecific binding &mdash; </strong>conjugated to highly cross-adsorbed antibodies</li> </ul> <h2 class="descHeader">Product Options</h2> <p>StarBright Blue 700 Secondary Antibodies can be used for <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/triplex-blot-image-desc.html&quot;);">multiplex fluorescent western blotting</a> with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/antibody/product_detail/description/global/detection-post-translational-modifications-desc.html&quot;);">detect post-translational modifications</a> like phosphorylation of a target protein.</p> <p><strong>Note:</strong> StarBright Antibodies are not suitable for stripping and reprobing.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"> <p><strong>Products</strong><strong> </strong></p> </td> <td align="center" valign="top"> <p><strong>Catalog Number</strong><strong> </strong></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004159">12004159 </a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004158">12004158</a></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Mouse IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004157">12004157</a></p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 80 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004162">12004162</a></p> </td> </tr> <tr class="pd_colorbackground"> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 400 &micro;l</p> </td> <td align="center" valign="top"> <p>12004161</p> </td> </tr> <tr> <td valign="top"> <p>StarBright Blue 700 Goat Anti-Rabbit IgG, 5 x 400 &micro;l</p> </td> <td align="center" valign="top"> <p><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=12004160">12004160</a></p> </td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/hfab-rhodamine-housekeeping-protein-fluorescent-primary-antibodies?ID=OKNWY3KG4">hFAB&trade; Rhodamine Housekeeping Protein Fluorescent Primary Antibodies</a></li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Primary Antibodies</a></li> <li><a href="/en-us/product/prestained-protein-standards">Precision Plus Protein&trade; Prestained Protein Standards</a></li> <li><a href="/en-us/product/chemidoc-mp-imaging-system">ChemiDoc MP Imaging System</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-ca/category/fluorescent-western-blotting-antibodies">Fluorescent Western Blotting Antibodies</a></li> <li><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade; Precast Gels</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>StarBright Blue 700 Fluorescent Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000066837.pdf" target="_blank">Instruction Manual</a><a href="/webroot/web/pdf/lsr/literature/10000078050.pdf" target="_blank"></a></li> <li>Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/10000068973.pdf" target="_blank">Selection Guide</a></li> <li>StarBright Blue 700 Secondary Antibodies <a href="/webroot/web/pdf/lsr/literature/Bulletin_6911.pdf" target="_blank">Flier</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="http://www.bioradiations.com/the-fluorescent-way-why-start-fluorescent-western-blotting-now" target="_blank">The Fluorescent Way: Why Start Fluorescent Western Blotting Now?</a></li> <li><a href="http://www.bioradiations.com/?s=Helpful+Tips+for+Optimizing+Your+Protocol" target="_blank">Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol</a></li> </ul> /webroot/web/html/lsr/products/antibody/sku_view/description/global starbright-blue-700-goat-anti-rabbit-igg-400-desc.html /webroot/web/html/lsr/products/antibody/sku_view/description/global/starbright-blue-700-goat-anti-rabbit-igg-400-desc.html Shelf life 2149 1 year at &ndash;20&deg;C lyophilized; 6 months at 4&deg;C after resuspension 1 0 Recommended dilution 3036 1:2,500&ndash;1:5,000 1 0 Cross-adsorbed species 3038 Bovine, goat, human, mouse, and rat 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004161&CartQuote=QT&quantity= false true false $103.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004161&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12004161&quantity= 12004161
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StarBright™ Blue 700 Goat Anti-Rabbit IgG, 5 x 400 µl

12004160
Pkg of 5, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 Fluorophore; reconstitute to 400 µl

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Number Description Options
10000066837 StarBright™ Blue 700 Fluorescent Secondary Antibodies Instruction Manual, Ver B Click to download
10000078050 Instruction Manual, StarBright™ Blue 700 Secondary Antibodies and hFAB™ Rhodamine Anti‑Housekeeping Protein Primary Antibodies Click to download
10000068973 Fluorescent Western Blotting with StarBright™ Blue 700 Secondary Antibodies Selection Guide, Ver A Click to download
6911 StarBright™ Blue 700 Secondary Antibodies Flier Click to download
M1721053 EIA Grade, Goat Anti-Rabbit IgG, Human IgG Adsorbed Instruction Manual (discontinued product, PDF only) Click to download

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