SsoFast™ EvaGreen® Supermix with Low ROX

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SsoFast EvaGreen Supermix With Low ROX

Overview

Sso7d Fusion Protein Technology
Bio-Rad’s patented* Sso7d fusion protein technology was first employed in the iProof™ high fidelity DNA polymerase product line. The unique Sso7d fusion polymerase in the SsoFast™ supermixes enables fast cycling without affecting PCR sensitivity, efficiency, and reproducibility. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Furthermore, this polymerase is significantly more resistant to PCR inhibitors, making the SsoFast  supermixes ideal choices for applications such as direct qPCR, without the need for sample preparation.
 
Fusing the double-stranded DNA binding protein Sso7d to DNA polymerases provides a powerful sliding grip on the replicated DNA.
 
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
Broad Dynamic Range Under Fast Cycling Conditions

The fusion polymerase in SsoFast™ EvaGreen® supermix with low ROX demonstrates a broad dynamic range under fast cycling conditions using the ABI 7500 fast real-time PCR system.


 

SsoFast EvaGreen supermix with low ROX generates consistent qPCR results on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions from 10 ng to 100 fg of cDNA were used in each 20 µl reaction. A, cDNA from human spleen was used to detect α-tubulin (α-tubulin efficiency = 105.8%, r = 0.996; total qPCR run time = 39 min, not including melt curve); B, cDNA from human liver was used to detect TIP (TIP efficiency = 92.7%, r = 0.992; total qPCR run time = 42 min, not including melt curve).

Broad Dynamic Range Over Six Orders of Magnitude

SsoFast™ EvaGreen® supermix with low ROX generates linear results over six orders of magnitide on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions of 100 ng to 100 fg of cDNA from HeLa total RNA were used in each 20 µl reaction to detect 18S rRNA. 18S rRNA efficiency = 98.6%, r2 = 0.999.

SsoFast™ EvaGreen® supermix with low ROX is a ROX-blended version of the high-performing SsoFast EvaGreen supermix that employs the patented Sso7d fusion protein technology for use in real-time PCR applications. This novel, engineered polymerase, combined with optimized buffer and EvaGreen dye, produces robust qPCR results in less time with increased speed, reliability, and sensitivity under fast thermal cycling conditions.

Key Features and Benefits

  • Fast qPCR results — Sso7d fusion polymerase's stringent hot-start and rapid polymerization kinetics provide consistent qPCR results under fast cycling conditions (approximately 40 min)
  • Preblended with ROX — streamlined use with real-time PCR systems that require ROX for fast runs
  • Minimal PCR inhibition — highly tolerant polymerase and EvaGreen dye exhibit low PCR inhibition and provide better performance with fast cycling protocols
  • Single copy detection — higher concentrations generate larger fluorescent signals to provide increased sensitivity
  • Broad dynamic range — data illustrate linear results over 6 orders of magnitude on real-time PCR systems

Applications and Uses

  • Dye-based qPCR detection
  • PCR and real-time PCR analysis
  • Nucleic acid amplification and expression profiling
  • ROX-dependent real-time PCR detection
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications
Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 6 months
Customer guarantee (minimum usage time) at –20°C 12 months
SsoFast EvaGreen Supermix with Low ROX

172-5210
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 200 x 20 µl reactions

List Price:   $153.00
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SsoFast EvaGreen Supermix with Low ROX

172-5211
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 500 x 20 µl reactions

List Price:   $343.00
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SsoFast EvaGreen Supermix with Low ROX

172-5212
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 1,000 x 20 µl reactions

List Price:   $650.00
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SsoFast EvaGreen Supermix with Low ROX

172-5213
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 2,000 x 20 µl reactions

List Price:   $1,276.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
5919 SsoFast EvaGreen Supermix with Low ROX Flier, Rev B Click to download
10016588 Instruction Manual, SsoFast™ EvaGreen® Supermix With Low ROX, Rev. B Click to download