SsoFast™ EvaGreen® Supermixes

Print
SsoFast EvaGreen Supermix

Overview

Sso7d Fusion Protein Technology
Bio-Rad’s patented* Sso7d fusion protein technology was first employed in the iProof™ high fidelity DNA polymerase product line. The unique Sso7d fusion polymerase in the SsoFast™ supermixes enables fast cycling without affecting PCR sensitivity, efficiency, and reproducibility. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Furthermore, this polymerase is significantly more resistant to PCR inhibitors, making the SsoFast  supermixes ideal choices for applications such as direct qPCR, without the need for sample preparation.
 
Fusing the double-stranded DNA binding protein Sso7d to DNA polymerases provides a powerful sliding grip on the replicated DNA.
 
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
Performance

The unique fusion polymerase in SsoFast EvaGreen supermix delivers extreme speed and generates exceptional qPCR results in less than 30 min. Tenfold serial dilutions of 10 ng to 100 ag of cDNA from human spleen were used in each 20 µl reaction to detect 18S RNA. 18S efficiency = 101.8%, r = 0.997. Total qPCR run time = 29 min. RFU, relative fluorescence units.

Sensitivity

SsoFast EvaGreen supermix provides extreme sensitivity in detection of a single copy of target gene. The ZAP70 gene was amplified and detected from fivefold serial dilutions of 10 ng to 80 pg () and 3.2 pg () of human genomic DNA. ZAP70 efficiency = 102.7%, r = 0.991. Insert shows the standard curve for the various dilutions. RFU, relative fluorescence units.

Reproducibility

Exceptional reproducibility can be achieved with SsoFast EvaGreen supermix. Efficient discrimination and reliable quantification can be obtained from 1.33-fold serial dilutions of input template. The CBP gene was amplified from varying amounts of human genomic DNA (5 ng to 500 pg). From left to right: () 5 ng, 2.83 ng, 1.60 ng, 903 pg, and 511 pg; () 3.76 ng, 2.13 ng, 1.20 ng, and 679 pg. CBP efficiency = 96.5%, r = 0.996. Insert is a magnified view showing robust discrimination and reproducible amplification. RFU, relative fluorescence units.

Bio-Rad introduces the SsoFast™ EvaGreen® supermix, a member of our next generation of real-time PCR supermixes using our patented Sso7d fusion protein technology, delivering a reagent that provides effective performance in a wide range of qPCR applications. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.

Key Features and Benefits

  • Fast qPCR results and high performance — the Sso7d fusion polymerase and optimized buffer deliver fast reaction times via instant polymerase activation and rapid polymerization kinetics to generate exceptional qPCR results in less than 30 min
  • Minimal inhibition of PCR — the polymerase's increased resistance to PCR inhibitors ensures maximum efficiency, sensitivity, and reproducibility, and provides increased fluorescence compared to SYBR® Green
  • Single copy detection — data illustrate high sensitivity with amplification and detection from a single copy of target gene
  • Robust discrimination and reproducibility — efficient discrimination and reliable quantification can be obtained from 1.33-fold serial dilutions of input template

Applications and Uses

  • Dye-based qPCR detection
  • PCR and real-time PCR analysis
  • Nucleic acid amplification and expression profiling
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications
Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 6 months
Customer guarantee (minimum usage time) at –20°C 12 months
SsoFast™ EvaGreen® Supermix

172-5200
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

List Price:   $153.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoFast™ EvaGreen® Supermix

172-5201
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 500 x 20 µl reactions

List Price:   $343.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoFast™ EvaGreen® Supermix

172-5202
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

List Price:   $650.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoFast™ EvaGreen® Supermix

172-5203
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,000 x 20 µl reactions

List Price:   $1,276.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoFast™ EvaGreen® Supermix

172-5204
25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions

List Price:   $1,506.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoFast™ EvaGreen® Supermix

172-5205
50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions

List Price:   $2,829.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
(Discontinued) SsoFast™ EvaGreen® Control Assay

172-5996
Predesigned kit for verifying performance of real-time PCR systems or training new users, includes template DNA, primers, supermix, water

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
10014647 Instruction Manual, SsoFast EvaGreen Supermix, Rev D Click to download
5816 SsoFast EvaGreen Supermix Flier, Rev B Click to download
5936 SsoFast EvaGreen Supermix Stability for Automated qPCR, Rev A Click to download
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]
10018778 SsoFast EvaGreen Control Assay Instructions, Rev A Click to download
5973 SsoFast EvaGreen Control Assay Flier, Rev A Click to download
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download [ Add to Cart (Free) ]