SsoFast™ EvaGreen® Supermixes

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Overview

Sso7d Fusion Protein Technology

Bio-Rad’s patented* Sso7d fusion protein technology was first employed in the iProof™ high fidelity DNA polymerase product line. The unique Sso7d fusion polymerase in the SsoFast™ Supermixes enables fast cycling without affecting PCR sensitivity, efficiency, and reproducibility. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Furthermore, this polymerase is significantly more resistant to PCR inhibitors, making the SsoFast  supermixes ideal choices for applications such as direct qPCR, without the need for sample preparation.

Fusing the double-stranded DNA binding protein Sso7d to DNA polymerases provides a powerful sliding grip on the replicated DNA.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.



Performance

The unique fusion polymerase in SsoFast EvaGreen Supermix delivers extreme speed and generates exceptional qPCR results in less than 30 min.

Tenfold serial dilutions of 10 ng to 100 ag of cDNA from human spleen were used in each 20 µl reaction to detect 18S RNA. 18S efficiency = 101.8%, r = 0.997. Total qPCR run time = 29 min. RFU, relative fluorescence units.



Sensitivity

SsoFast EvaGreen Supermix provides extreme sensitivity in detection of a single copy of target gene.

The ZAP70 gene was amplified and detected from fivefold serial dilutions of 10 ng to 80 pg () and 3.2 pg () of human genomic DNA. ZAP70 efficiency = 102.7%, r = 0.991. Insert shows the standard curve for the various dilutions. RFU, relative fluorescence units.



Reproducibility

Exceptional reproducibility can be achieved with SsoFast EvaGreen Supermix.

Efficient discrimination and reliable quantification can be obtained from 1.33-fold serial dilutions of input template. The CBP gene was amplified from varying amounts of human genomic DNA (5 ng to 500 pg). From left to right: () 5 ng, 2.83 ng, 1.60 ng, 903 pg, and 511 pg; () 3.76 ng, 2.13 ng, 1.20 ng, and 679 pg. CBP efficiency = 96.5%, r = 0.996. Insert is a magnified view showing robust discrimination and reproducible amplification. RFU, relative fluorescence units..





NEW PRODUCT
SsoAdvanced™ Universal Supermixes

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance under all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration
SsoAdvanced Universal Supermixes

SsoFast EvaGreen Supermix utilizes our patented Sso7d fusion protein technology* for high performance in a wide range of qPCR applications. The dsDNA-binding protein Sso7d stabilizes the polymerase-template complex, increasing processivity and reducing reaction times compared with conventional DNA polymerases without affecting PCR sensitivity, efficiency, or reproducibility. EvaGreen formulation provides increased fluorescence compared to SYBR® Green.

Key Features and Benefits

  • Fast qPCR results and high performance — instant activation and rapid polymerization kinetics generate exceptional qPCR results in less than 30 min
  • Minimal PCR inhibition — increased resistance to PCR inhibitors ensures maximum efficiency, sensitivity, and reproducibility
  • Single-copy detection — provides high sensitivity with amplification and detection from a single copy of a target gene
  • Robust discrimination and reproducibility — achieves efficient discrimination and reliable quantification from 1.33-fold serial dilutions of template

Applications and Uses

  • Dye-based qPCR detection
  • Gene expression analysis
  • Genotyping studies

Related Products

Select the best real-time PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase

Key Features of Bio-Rad Real-Time PCR Supermixes

SsoFast™
Supermixes

SsoAdvanced™ Universal Supermixes

Sso7d fusion protein technology

Yes

Yes

Sensitive detection of low-level targets

Efficiency with difficult target sequences

Specificity

Tolerance to PCR inhibitors

Broad range of reaction conditions

Fast cycling

Instrument compatibility

Select instruments

Any instrument


Related Categories

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 6 months
Customer guarantee (minimum usage time) at –20°C 12 months
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SsoFast™ EvaGreen® Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725200
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

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$180.00
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SsoFast™ EvaGreen® Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725201
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 500 x 20 µl reactions

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$395.00
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SsoFast™ EvaGreen® Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725202
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

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$745.00
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SsoFast™ EvaGreen® Supermix, 2,000 x 20 µl rxns, 20 ml (1 x 20 ml)

1725203
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,000 x 20 µl reactions

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$1,465.00
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SsoFast™ EvaGreen® Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725204
25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions

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$1,730.00
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SsoFast™ EvaGreen® Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725205
50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions

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$3,240.00
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(Discontinued) SsoFast™ EvaGreen® Control Assay

1725996
Predesigned kit for verifying performance of real-time PCR systems or training new users, includes template DNA, primers, supermix, water

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure, Rev E Click to download [ Add to Cart (Free) ]
10014647 Instruction Manual, SsoFast EvaGreen Supermix, Rev D Click to download
5816 SsoFast™ EvaGreen® Supermix Flier, Rev B Click to download
5936 SsoFast EvaGreen Supermix Stability for Automated qPCR, Rev A Click to download
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]
10018778 SsoFast™ EvaGreen Control Assay Instructions, Rev A Click to download
5973 SsoFast EvaGreen Control Assay Flier, Rev A Click to download
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download
6773 Real-Time PCR Supermixes Flier, Ver A Click to download