SsoFast™ EvaGreen® Supermix with Low ROX

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Overview

Sso7d Fusion Protein Technology

Bio-Rad’s patented* Sso7d fusion protein technology was first employed in the iProof™ high fidelity DNA polymerase product line. The unique Sso7d fusion polymerase in the SsoFast™ Supermixes enables fast cycling without affecting PCR sensitivity, efficiency, and reproducibility. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Furthermore, this polymerase is significantly more resistant to PCR inhibitors, making the SsoFast  supermixes ideal choices for applications such as direct qPCR, without the need for sample preparation.

Fusing the double-stranded DNA binding protein Sso7d to DNA polymerases provides a powerful sliding grip on the replicated DNA.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.



Broad Dynamic Range Under Fast Cycling Conditions

The fusion polymerase in SsoFast™ EvaGreen® Supermix with Low ROX demonstrates a broad dynamic range under fast cycling conditions using the ABI 7500 fast Real-Time PCR System.

SsoFast EvaGreen Supermix with Low ROX generates consistent qPCR results on the ABI 7500 fast Real-Time PCR System. Tenfold serial dilutions from 10 ng to 100 fg of cDNA were used in each 20 µl reaction.

cDNA from human spleen was used to detect α-tubulin (α-tubulin efficiency = 105.8%, r = 0.996; total qPCR run time = 39 min, not including melt curve).



cDNA from human liver was used to detect TIP (TIP efficiency = 92.7%, r = 0.992; total qPCR run time = 42 min, not including melt curve).



Broad Dynamic Range Over Six Orders of Magnitude

SsoFast™ EvaGreen® Supermix with low ROX generates linear results over six orders of magnitide on the ABI 7500 fast real-time PCR system.

Tenfold serial dilutions of 100 ng to 100 fg of cDNA from HeLa total RNA were used in each 20 µl reaction to detect 18S rRNA. 18S rRNA efficiency = 98.6%, r2 = 0.999.





NEW PRODUCT
SsoAdvanced™ Universal Supermixes

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance under all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration
SsoAdvanced Universal Supermixes

SsoFast EvaGreen Supermix with Low ROX is a ROX-blended version of our high-performing SsoFast EvaGreen Supermix, which employs the patented Sso7d fusion protein technology* for use in real-time PCR applications. This novel, engineered polymerase, combined with optimized buffer and EvaGreen dye, produces robust qPCR results in less time with increased speed, reliability, and sensitivity under fast thermal cycling conditions.

Key Features and Benefits

  • Fast qPCR results — Sso7d fusion polymerase's stringent hot-start and rapid polymerization kinetics provide consistent qPCR results under fast cycling conditions (approximately 40 min)
  • Preblended with ROX — streamlines use with real-time PCR systems that require ROX for fast runs
  • Minimal PCR inhibition — highly tolerant polymerase and EvaGreen dye exhibit low PCR inhibition and provide better performance with fast cycling protocols
  • Single-copy detection — higher concentrations generate larger fluorescent signals to provide increased sensitivity
  • Broad dynamic range — yields linear results over 6 orders of magnitude on real-time PCR systems

Applications and Uses

  • Dye-based qPCR detection
  • PCR and real-time PCR analysis
  • ROX-dependent real-time PCR detection
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications

Related Products

Select the best real-tme PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase

Key Features of Bio-Rad Real-Time PCR Supermixes

SsoFast™
Supermixes

SsoAdvanced™ Universal Supermixes

Sso7d fusion protein technology

Yes

Yes

Sensitive detection of low-level targets

Efficiency with difficult target sequences

Specificity

Tolerance to PCR inhibitors

Broad range of reaction conditions

Fast cycling

Instrument compatibility

Select instruments

Any instrument


Related Categories

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 6 months
Customer guarantee (minimum usage time) at –20°C 12 months
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SsoFast™ EvaGreen® Supermix with Low ROX, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725210
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 200 x 20 µl reactions

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$170.00
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SsoFast™ EvaGreen® Supermix with Low ROX, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725211
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 500 x 20 µl reactions

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$375.00
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SsoFast™ EvaGreen® Supermix with Low ROX, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725212
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 1,000 x 20 µl reactions

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$705.00
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SsoFast™ EvaGreen® Supermix with Low ROX, 2,000 x 20 µl rxns, 20 ml (1 x 20 ml)

1725213
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 2,000 x 20 µl reactions

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$1,380.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure, Rev E Click to download [ Add to Cart (Free) ]
5919 SsoFast EvaGreen Supermix with Low ROX Flier, Rev B Click to download
10016588 Instruction Manual, SsoFast™ EvaGreen® Supermix With Low ROX, Rev. B Click to download
6773 Real-Time PCR Supermixes Flier, Ver A Click to download