SsoAdvanced™ Universal SYBR® Green Supermix

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SsoAdvanced Universal SYBR Green Supermix

Overview

Exclusive Sso7d Fusion Polymerase Technology to Empower Your PCR

Sso7d, a 7 kD protein obtained from Sulfolobus solfataricus, binds to dsDNA without any sequence specificity.

Sso7d is covalently linked to a DNA polymerase and is able to stabilize the polymerase-template complex without compromising the structural integrity, thermal stability, or catalytic activity of the enzyme.

The unique structure of this Sso7d fusion polymerase provides several significant advantages over ordinary DNA polymerase:

  • Increased processivity
  • Effective amplification in GC-rich regions or strong secondary structure
  • Efficient amplification of longer products
  • Superior tolerance to PCR inhibitors
Sso7D Technology
PCR Inhibitor Tolerance

Superior qPCR Performance in the Presence of an Inhibitor

An ADAR qPCR assay with an input of 5 ng cDNA was performed using SsoAdvanced™ Universal SYBR® Green Supermix and SYBR GreenER® qPCR Supermix Universal with and without Eagle’s minimum essential medium (EMEM) supplemented with 10% fetal bovine serum. 10, 20, and 40% EMEM supplemented with 10% fetal bovine serum was spiked in the cDNA to act as an inhibitor. SsoAdvanced Universal SYBR® Green Supermix showed less Cq delay. Cq, quantification cycle.



GC-Rich and AT-Rich qPCR

Excellent qPCR Efficiency and Sensitivity Even with Difficult Target Sequences

qPCR reactions were performed with GC- and AT-rich assays using SsoAdvanced™ Universal SYBR® Green Supermix. Great linearity and efficiency was achieved in both assays, indicating exceptional performance even with challenging assays.

61% GC-rich TIP assay with an input of 50 ng–5 pg gDNA produced standard curve R2 = 0.997, efficiency = 102%, and slope = –3.27



71% AT-rich ADAR sequence with an input of 10 ng–10 pg cDNA produced standard curve R2 = 0.999, efficiency = 92%, and slope = –3.52.



PrimePCR Assay Compatibility

Validated to Generate Best-Quality Data with a PrimePCR Assay

A PrimePCR assay targeting β2 microglobulin with an input of 100 ng–1 pg gDNA was performed using SsoAdvanced™ Universal SYBR® Green Supermix. Results demonstrated outstanding reproducibility and sensitivity over six orders of magnitude. RFU, relative fluorescence units.



Sensitivity and Specificity

Maximum PCR Sensitivity and Specificity

A PrimePCR™ Assay targeting β2 microglobulin, using 250, 125, 62.5, 31.25, 15.625, or 7.8125 cells per 20 µl reaction was performed using SsoAdvanced™ Universal SYBR® Green Supermix. Results show a linear response to varying target concentrations, indicating reliable and effective discrimination of twofold dilutions.



A melt curve analysis of the above reactions shows uniform curves with single peaks, confirming the absence of nonspecific PCR products. RFU, relative fluorescence units.



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Real-Time PCR Reagents App

In-depth product information, performance data, interactive tutorials, videos, and more.

SsoAdvanced™ Universal SYBR® Green Supermix is an exclusive high-performance real-time PCR reagent based on Bio-Rad's patented* Sso7d fusion protein polymerase technology and advanced buffer formulation.

This supermix is uniquely formulated to provide higher processivity, increased PCR inhibitor tolerance, and robust performance with challenging templates and targets in SYBR® Green-based qPCR. It delivers superior performance for a wide range of applications on any qPCR platform and is validated to ensure optimal results with Bio-Rad’s PrimePCR™ Gene Expression Assays.

SsoAdvanced™ Universal SYBR® Green Supermix allows you to:

  • Achieve superior real-time PCR results under various experimental conditions — for example, primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the highly processive Sso7d fusion polymerase has increased resistance to various PCR inhibitors, enhancing sensitivity and overall performance
  • Shorten time to results while maintaining qPCR data quality — the Sso7d fusion polymerase and optimized buffer provide rapid polymerization kinetics and instant polymerase activation
  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system

Applications and Uses of SsoAdvanced™ Universal SYBR® Green Supermix

Instrument Compatibility

SsoAdvanced™ Universal SYBR® Green Supermix is compatible with all commercially available and all Bio-Rad Real-Time qPCR Systems.

Related Products

Bio-Rad offers complete workflow solutions for real-time PCR:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.



* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 3 months
Customer guarantee (minimum usage time) at –20°C 12 months
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SsoAdvanced™ Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725270
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions

1725270 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5270_1361992326.xml 1725270 02/27/13 11:09 AM en_US true internet/product_SKU 2018-02-27 06:49:38 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5270_1361992326.xml /default/main/bio-rad/WORKAREA/default jcrucyqa 2017-11-14 07:07:37 true jcrucyqa templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5270_1361992326.xml LSR N /default/main/bio-rad en 02/20/27 11:26 AM 1519172644938 2018-02-27 06:49:38 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5270_1361992326.xml 1725270-ssoadvanced-universal-sybr-green-supermix-200-x-20-ul-rxns-2-ml-2-x-1-ml 1 1725270 SsoAdvanced Universal SYBR Green Supermix SsoAdvanced™ Universal SYBR<sup>®</sup> Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml) 2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 200 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-sybr-green-supermix-172-5270-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-sybr-green-supermix-172-5270-view.jpg Molly Gregg SsoAdvanced Universal SYBR Green Supermix 2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 200 x 20 &micro;l reactions 172-5270, 1725270, ssoadvanced, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase, rox 02/27/13 11:09 AM 02/20/27 11:26 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>, stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">superior inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>, specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">sensitivity</a>, efficiency, or reproducibility.</p> <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix lets you:</p> <ul> <li><strong>Use any real-time PCR system</strong> &mdash; the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system</li> <li><strong>Achieve superior real-time PCR results under various conditions</strong> &mdash; our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges</li> <li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised samples</strong> &mdash; the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">increased resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">better sensitivity</a> and overall performance</li> <li><strong>Decrease time to results without compromising qPCR data quality</strong> &mdash; the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation</li> <li><strong>Obtain better results with Bio-Rad's <a style="font-weight: bold;" href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance-tutorial.html');">PrimePCR&trade; assays</a></strong> &mdash; real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance</li> </ul> <h2 class="descHeader">Applications and Uses of SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</h2> <ul> <li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li> <li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li> <li><a href="http://www.bio-rad.com/evportal/en/US/LSR/PathwayLanding/PHP-4D41C030-E3D3-4382-A49C-A1A565D28D0E/homepage/PrimePCR_Pathways">Pathway analysis</a></li> <li>Absolute quantification</li> <li>Chromatin immunoprecipitation (ChIP) qPCR</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Characterization of genetically modified organisms (GMO)</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="font-size: 12px; font-weight: bold;">Number of 20 &mu;l Reactions</td> <td style="font-size: 12px; font-weight: bold;">Total Volume</td> <td style="font-size: 12px; font-weight: bold;">Number of Vials</td> <td style="font-size: 12px; font-weight: bold;">Catalog #</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;&nbsp;200</td> <td>&nbsp;&nbsp;2 ml</td> <td>&nbsp;&nbsp;2 x 1 ml</td> <td><strong style="font-size: 12px;">#172-5270</strong></td> </tr> <tr> <td>&nbsp;&nbsp;&nbsp;500</td> <td>&nbsp;&nbsp;5 ml</td> <td>&nbsp;&nbsp;5 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5271">#172-5271</a></td> </tr> <tr class="pd_colorbackground"> <td>1,000</td> <td>10 ml</td> <td>10 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5272">#172-5272</a></td> </tr> <tr> <td>2,500</td> <td>25 ml</td> <td>&nbsp;&nbsp;5 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5274">#172-5274</a></td> </tr> <tr class="pd_colorbackground"> <td>5,000</td> <td>50 ml</td> <td>10 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5275">#172-5275</a></td> </tr> </tbody> </table> <p>&nbsp;</p> <h2 class="descHeader">Instrument Compatibility</h2> <p>The SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is compatible with all commercially available and all <a href="/evportal/destination/product?catID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad real-time qPCR systems</a>.</p> <h2 class="descHeader">Related Products</h2> <p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H424VY">SsoAdvanced universal probes supermix</a>, which is available in several formats (200&ndash;5,000 reactions).</p> <p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p> <ul> <li><a href="/evportal/destination/product?catID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA isolation kits</a></li> <li><a href="/evportal/destination/product?catID=M87EPGE8Z">Reverse transcription kits</a></li> <li><a href="/evportal/destination/product?catID=M87F1J8UU">Real-time PCR supermixes</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf">troubleshooting reactions</a>.</p> <p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p> <p> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#pcrInhibitorTolerance').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#gcRich').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-gc-rich.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#geneCosRapidTrans').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/duplex-reaction.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#betterSensitivity').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html'); }); }); // ]]></script> </p> /webroot/web/html/lsr/products/SKUs 172-5270.html /webroot/web/html/lsr/products/SKUs/172-5270.html Product expiration (after shipping date) 2026 12 months 1 0 Storage at 4°C (after thawing) 2806 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 6286 templatedata/internet/documentation/data/LSR/Global/English/Literature/6286_en.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725270&CartQuote=QT&quantity= false true false $170.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725270&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725270&quantity= 1725270
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SsoAdvanced™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions

1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725271 02/27/13 11:09 AM en_US true internet/product_SKU 2018-02-27 06:49:41 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml /default/main/bio-rad/WORKAREA/default jcihuc6i 2017-11-14 07:07:37 true jcihuc6i templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml LSR N /default/main/bio-rad en 02/27/23 11:15 AM 1519353028915 2018-02-27 06:49:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725271-ssoadvanced-universal-sybr-green-supermix-500-x-20-ul-rxns-5-ml-5-x-1-ml 1 1725271 SsoAdvanced Universal SYBR Green Supermix SsoAdvanced™ Universal SYBR<sup>®</sup> Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml) 5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX Normalization Dyes, for 500 x 20 &micro;l reactions 5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX Normalization Dyes, for 500 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-sybr-green-supermix-172-5271-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-sybr-green-supermix-172-5271-view.jpg Molly Gregg SsoAdvanced Universal SYBR Green Supermix 5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX Normalization Dyes, for 500 x 20 &micro;l reactions 172-5271, 1725271, ssoadvanced, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase, rox 02/27/13 11:09 AM 02/27/23 11:15 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>, stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">superior inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>, specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">sensitivity</a>, efficiency, or reproducibility.</p> <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix lets you:</p> <ul> <li><strong>Use any real-time PCR system</strong> &mdash; the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system</li> <li><strong>Achieve superior real-time PCR results under various conditions</strong> &mdash; our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges</li> <li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised samples</strong> &mdash; the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">increased resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">better sensitivity</a> and overall performance</li> <li><strong>Decrease time to results without compromising qPCR data quality</strong> &mdash; the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation</li> <li><strong>Obtain better results with Bio-Rad's <a style="font-weight: bold;" href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance-tutorial.html');">PrimePCR&trade; assays</a></strong> &mdash; real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance</li> </ul> <h2 class="descHeader">Applications and Uses of SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</h2> <ul> <li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li> <li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li> <li><a href="http://www.bio-rad.com/evportal/en/US/LSR/PathwayLanding/PHP-4D41C030-E3D3-4382-A49C-A1A565D28D0E/homepage/PrimePCR_Pathways">Pathway analysis</a></li> <li>Absolute quantification</li> <li>Chromatin immunoprecipitation (ChIP) qPCR</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Characterization of genetically modified organisms (GMO)</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="font-size: 12px; font-weight: bold;">Number of 20 &mu;l Reactions</td> <td style="font-size: 12px; font-weight: bold;">Total Volume</td> <td style="font-size: 12px; font-weight: bold;">Number of Vials</td> <td style="font-size: 12px; font-weight: bold;">Catalog #</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;&nbsp;200</td> <td>&nbsp;&nbsp;2 ml</td> <td>&nbsp;&nbsp;2 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5270">#172-5270</a></td> </tr> <tr> <td>&nbsp;&nbsp;&nbsp;500</td> <td>&nbsp;&nbsp;5 ml</td> <td>&nbsp;&nbsp;5 x 1 ml</td> <td><strong style="font-size: 12px;">#172-5271</strong></td> </tr> <tr class="pd_colorbackground"> <td>1,000</td> <td>10 ml</td> <td>10 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5272">#172-5272</a></td> </tr> <tr> <td>2,500</td> <td>25 ml</td> <td>&nbsp;&nbsp;5 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5274">#172-5274</a></td> </tr> <tr class="pd_colorbackground"> <td>5,000</td> <td>50 ml</td> <td>10 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5275">#172-5275</a></td> </tr> </tbody> </table> <p>&nbsp;</p> <h2 class="descHeader">Instrument Compatibility</h2> <p>The SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is compatible with all commercially available and all <a href="/evportal/destination/product?catID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad real-time qPCR systems</a>.</p> <h2 class="descHeader">Related Products</h2> <p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H424VY">SsoAdvanced universal probes supermix</a>, which is available in several formats (200&ndash;5,000 reactions).</p> <p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p> <ul> <li><a href="/evportal/destination/product?catID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA isolation kits</a></li> <li><a href="/evportal/destination/product?catID=M87EPGE8Z">Reverse transcription kits</a></li> <li><a href="/evportal/destination/product?catID=M87F1J8UU">Real-time PCR supermixes</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf">troubleshooting reactions</a>.</p> <p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p> <p> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#pcrInhibitorTolerance').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#gcRich').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-gc-rich.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#geneCosRapidTrans').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/duplex-reaction.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#betterSensitivity').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html'); }); }); // ]]></script> </p> /webroot/web/html/lsr/products/SKUs 172-5271.html /webroot/web/html/lsr/products/SKUs/172-5271.html Product expiration (after shipping date) 2026 12 months 1 0 Storage at 4°C (after thawing) 2806 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725271&CartQuote=QT&quantity= false true false $376.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725271&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725271&quantity= 1725271
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SsoAdvanced™ Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725272
10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 1,000 x 20 µl reactions

1725272 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5272_1362002187.xml 1725272 02/27/13 12:23 PM en_US true internet/product_SKU 2018-02-27 06:49:39 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5272_1362002187.xml /default/main/bio-rad/WORKAREA/default jcrucysi 2017-11-14 07:07:37 true jcrucysi templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5272_1362002187.xml LSR N /default/main/bio-rad en 02/27/23 01:55 PM 1519353028958 2018-02-27 06:49:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5272_1362002187.xml 1725272-ssoadvanced-universal-sybr-green-supermix-1000-x-20-ul-rxns-10-ml-10-x-1-ml 1 1725272 SsoAdvanced Universal SYBR Green Supermix SsoAdvanced™ Universal SYBR<sup>®</sup> Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) 10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 1,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-sybr-green-supermix-172-5272-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-sybr-green-supermix-172-5272-view.jpg Molly Gregg SsoAdvanced Universal SYBR Green Supermix 10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 1,000 x 20 &micro;l reactions 172-5272, 1725272, ssoadvanced, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase, rox 02/27/13 12:23 PM 02/27/23 01:55 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>, stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">superior inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>, specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">sensitivity</a>, efficiency, or reproducibility.</p> <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix lets you:</p> <ul> <li><strong>Use any real-time PCR system</strong> &mdash; the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system</li> <li><strong>Achieve superior real-time PCR results under various conditions</strong> &mdash; our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges</li> <li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised samples</strong> &mdash; the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">increased resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">better sensitivity</a> and overall performance</li> <li><strong>Decrease time to results without compromising qPCR data quality</strong> &mdash; the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation</li> <li><strong>Obtain better results with Bio-Rad's <a style="font-weight: bold;" href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance-tutorial.html');">PrimePCR&trade; assays</a></strong> &mdash; real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance</li> </ul> <h2 class="descHeader">Applications and Uses of SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</h2> <ul> <li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li> <li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li> <li><a href="http://www.bio-rad.com/evportal/en/US/LSR/PathwayLanding/PHP-4D41C030-E3D3-4382-A49C-A1A565D28D0E/homepage/PrimePCR_Pathways">Pathway analysis</a></li> <li>Absolute quantification</li> <li>Chromatin immunoprecipitation (ChIP) qPCR</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Characterization of genetically modified organisms (GMO)</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="font-size: 12px; font-weight: bold;">Number of 20 &mu;l Reactions</td> <td style="font-size: 12px; font-weight: bold;">Total Volume</td> <td style="font-size: 12px; font-weight: bold;">Number of Vials</td> <td style="font-size: 12px; font-weight: bold;">Catalog #</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;&nbsp;200</td> <td>&nbsp;&nbsp;2 ml</td> <td>&nbsp;&nbsp;2 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5270">#172-5270</a></td> </tr> <tr> <td>&nbsp;&nbsp;&nbsp;500</td> <td>&nbsp;&nbsp;5 ml</td> <td>&nbsp;&nbsp;5 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5271">#172-5271</a></td> </tr> <tr class="pd_colorbackground"> <td>1,000</td> <td>10 ml</td> <td>10 x 1 ml</td> <td><strong style="font-size: 12px;">#172-5272</strong></td> </tr> <tr> <td>2,500</td> <td>25 ml</td> <td>&nbsp;&nbsp;5 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5274">#172-5274</a></td> </tr> <tr class="pd_colorbackground"> <td>5,000</td> <td>50 ml</td> <td>10 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5275">#172-5275</a></td> </tr> </tbody> </table> <p>&nbsp;</p> <h2 class="descHeader">Instrument Compatibility</h2> <p>The SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is compatible with all commercially available and all <a href="/evportal/destination/product?catID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad real-time qPCR systems</a>.</p> <h2 class="descHeader">Related Products</h2> <p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H424VY">SsoAdvanced universal probes supermix</a>, which is available in several formats (200&ndash;5,000 reactions).</p> <p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p> <ul> <li><a href="/evportal/destination/product?catID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA isolation kits</a></li> <li><a href="/evportal/destination/product?catID=M87EPGE8Z">Reverse transcription kits</a></li> <li><a href="/evportal/destination/product?catID=M87F1J8UU">Real-time PCR supermixes</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf">troubleshooting reactions</a>.</p> <p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p> <p> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#pcrInhibitorTolerance').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#gcRich').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-gc-rich.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#geneCosRapidTrans').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/duplex-reaction.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#betterSensitivity').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html'); }); }); // ]]></script> </p> /webroot/web/html/lsr/products/SKUs 172-5272.html /webroot/web/html/lsr/products/SKUs/172-5272.html Product expiration (after shipping date) 2026 12 months 1 0 Storage at 4°C (after thawing) 2806 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725272&CartQuote=QT&quantity= false true false $721.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725272&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725272&quantity= 1725272
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SsoAdvanced™ Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725274
25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 2,500 x 20 µl reactions

1725274 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5274_1362002284.xml 1725274 02/27/13 12:23 PM en_US true internet/product_SKU 2018-02-27 06:49:40 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5274_1362002284.xml /default/main/bio-rad/WORKAREA/default jcrucyqz 2017-11-14 07:07:37 true jcrucyqz templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5274_1362002284.xml LSR N /default/main/bio-rad en 02/27/23 01:57 PM 1519353029004 2018-02-27 06:49:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5274_1362002284.xml 1725274-ssoadvanced-universal-sybr-green-supermix-2500-x-20-ul-rxns-25-ml-5-x-5-ml 1 1725274 SsoAdvanced Universal SYBR Green Supermix SsoAdvanced™ Universal SYBR<sup>®</sup> Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml) 25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 2,500 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-sybr-green-supermix-172-5274-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-sybr-green-supermix-172-5274-view.jpg Molly Gregg SsoAdvanced Universal SYBR Green Supermix 25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 2,500 x 20 &micro;l reactions 172-5274, 1725274, ssoadvanced, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase, rox 02/27/13 12:23 PM 02/27/23 01:57 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>, stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">superior inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>, specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">sensitivity</a>, efficiency, or reproducibility.</p> <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix lets you:</p> <ul> <li><strong>Use any real-time PCR system</strong> &mdash; the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system</li> <li><strong>Achieve superior real-time PCR results under various conditions</strong> &mdash; our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges</li> <li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised samples</strong> &mdash; the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance.html');">increased resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sensitivity.html');">better sensitivity</a> and overall performance</li> <li><strong>Decrease time to results without compromising qPCR data quality</strong> &mdash; the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation</li> <li><strong>Obtain better results with Bio-Rad's <a style="font-weight: bold;" href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/pcr-inhibitor-tolerance-tutorial.html');">PrimePCR&trade; assays</a></strong> &mdash; real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance</li> </ul> <h2 class="descHeader">Applications and Uses of SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</h2> <ul> <li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li> <li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li> <li><a href="http://www.bio-rad.com/evportal/en/US/LSR/PathwayLanding/PHP-4D41C030-E3D3-4382-A49C-A1A565D28D0E/homepage/PrimePCR_Pathways">Pathway analysis</a></li> <li>Absolute quantification</li> <li>Chromatin immunoprecipitation (ChIP) qPCR</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Characterization of genetically modified organisms (GMO)</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="font-size: 12px; font-weight: bold;">Number of 20 &mu;l Reactions</td> <td style="font-size: 12px; font-weight: bold;">Total Volume</td> <td style="font-size: 12px; font-weight: bold;">Number of Vials</td> <td style="font-size: 12px; font-weight: bold;">Catalog #</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;&nbsp;200</td> <td>&nbsp;&nbsp;2 ml</td> <td>&nbsp;&nbsp;2 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5270">#172-5270</a></td> </tr> <tr> <td>&nbsp;&nbsp;&nbsp;500</td> <td>&nbsp;&nbsp;5 ml</td> <td>&nbsp;&nbsp;5 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5271">#172-5271</a></td> </tr> <tr class="pd_colorbackground"> <td>1,000</td> <td>10 ml</td> <td>10 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5272">#172-5272</a></td> </tr> <tr> <td>2,500</td> <td>25 ml</td> <td>&nbsp;&nbsp;5 x 5 ml</td> <td><strong style="font-size: 12px;">#172-5274</strong></td> </tr> <tr class="pd_colorbackground"> <td>5,000</td> <td>50 ml</td> <td>10 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5275">#172-5275</a></td> </tr> </tbody> </table> <p>&nbsp;</p> <h2 class="descHeader">Instrument Compatibility</h2> <p>The SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is compatible with all commercially available and all <a href="/evportal/destination/product?catID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad real-time qPCR systems</a>.</p> <h2 class="descHeader">Related Products</h2> <p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H424VY">SsoAdvanced universal probes supermix</a>, which is available in several formats (200&ndash;5,000 reactions).</p> <p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p> <ul> <li><a href="/evportal/destination/product?catID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA isolation kits</a></li> <li><a href="/evportal/destination/product?catID=M87EPGE8Z">Reverse transcription kits</a></li> <li><a href="/evportal/destination/product?catID=M87F1J8UU">Real-time PCR supermixes</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf">troubleshooting reactions</a>.</p> <p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p> <p> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#pcrInhibitorTolerance').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#gcRich').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-gc-rich.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#geneCosRapidTrans').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/duplex-reaction.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#betterSensitivity').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html'); }); }); // ]]></script> </p> /webroot/web/html/lsr/products/SKUs 172-5274.html /webroot/web/html/lsr/products/SKUs/172-5274.html Product expiration (after shipping date) 2026 12 months 1 0 Storage at 4°C (after thawing) 2806 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725274&CartQuote=QT&quantity= false true false $1,669.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725274&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725274&quantity= 1725274
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SsoAdvanced™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725275
50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 5,000 x 20 µl reactions

1725275 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5275_1362002377.xml 1725275 02/27/13 01:58 PM en_US true internet/product_SKU 2018-02-27 06:49:39 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5275_1362002377.xml /default/main/bio-rad/WORKAREA/default j9q3h4h0 2017-10-27 13:56:05 true j9q3h4h0 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5275_1362002377.xml LSR N /default/main/bio-rad en 02/27/23 01:58 PM 1519353029063 2018-02-27 06:49:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5275_1362002377.xml 1725275-ssoadvanced-universal-sybr-green-supermix-5000-x-20-ul-rxns-50-ml-10-x-5-ml 1 1725275 SsoAdvanced Universal SYBR Green Supermix SsoAdvanced™ Universal SYBR<sup>®</sup> Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml) 50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 5,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-sybr-green-supermix-172-5275-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-sybr-green-supermix-172-5275-view.jpg Molly Gregg SsoAdvanced Universal SYBR Green Supermix 50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, SYBR<sup>&reg;</sup> Green I, ROX normalization dyes, for 5,000 x 20 &micro;l reactions 172-5275, 1725275, ssoadvanced, universal, sybr, green, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase, rox 02/27/13 01:58 PM 02/27/23 01:58 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a>Sso7d</a>, stabilizes the polymerase-template complex, providing <a>superior inhibitor tolerance</a>, increased <a>processivity</a>, specificity, and greater speed without affecting PCR <a>sensitivity</a>, efficiency, or reproducibility.</p> <p>SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix lets you:</p> <ul> <li><strong>Use any real-time PCR system</strong> &mdash; the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system</li> <li><strong>Achieve superior real-time PCR results under various conditions</strong> &mdash; our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges</li> <li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised samples</strong> &mdash; the Sso7d fusion polymerase has <a>increased resistance</a> to a wide variety of PCR inhibitors, which helps provide <a>better sensitivity</a> and overall performance</li> <li><strong>Decrease time to results without compromising qPCR data quality</strong> &mdash; the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation</li> <li><strong>Obtain better results with Bio-Rad's <a style="font-weight: bold;">PrimePCR&trade; assays</a></strong> &mdash; real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance</li> </ul> <h2 class="descHeader">Applications and Uses of SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</h2> <ul> <li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li> <li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li> <li><a href="http://www.bio-rad.com/evportal/en/US/LSR/PathwayLanding/PHP-4D41C030-E3D3-4382-A49C-A1A565D28D0E/homepage/PrimePCR_Pathways">Pathway analysis</a></li> <li>Absolute quantification</li> <li>Chromatin immunoprecipitation (ChIP) qPCR</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Characterization of genetically modified organisms (GMO)</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="font-size: 12px; font-weight: bold;">Number of 20 &mu;l Reactions</td> <td style="font-size: 12px; font-weight: bold;">Total Volume</td> <td style="font-size: 12px; font-weight: bold;">Number of Vials</td> <td style="font-size: 12px; font-weight: bold;">Catalog #</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;&nbsp;200</td> <td>&nbsp;&nbsp;2 ml</td> <td>&nbsp;&nbsp;2 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5270">#172-5270</a></td> </tr> <tr> <td>&nbsp;&nbsp;&nbsp;500</td> <td>&nbsp;&nbsp;5 ml</td> <td>&nbsp;&nbsp;5 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5271">#172-5271</a></td> </tr> <tr class="pd_colorbackground"> <td>1,000</td> <td>10 ml</td> <td>10 x 1 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5272">#172-5272</a></td> </tr> <tr> <td>2,500</td> <td>25 ml</td> <td>&nbsp;&nbsp;5 x 5 ml</td> <td><a style="font-weight: bold;" href="/evportal/destination/commerce/sku_detail?productID=172-5274">#172-5274</a></td> </tr> <tr class="pd_colorbackground"> <td>5,000</td> <td>50 ml</td> <td>10 x 5 ml</td> <td><strong style="font-size: 12px;">#172-5275</strong></td> </tr> </tbody> </table> <p>&nbsp;</p> <h2 class="descHeader">Instrument Compatibility</h2> <p>The SsoAdvanced&trade; universal SYBR<sup>&reg;</sup> Green supermix is compatible with all commercially available and all <a href="/evportal/destination/product?catID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad real-time qPCR systems</a>.</p> <h2 class="descHeader">Related Products</h2> <p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H424VY">SsoAdvanced universal probes supermix</a>, which is available in several formats (200&ndash;5,000 reactions).</p> <p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p> <ul> <li><a href="/evportal/destination/product?catID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA isolation kits</a></li> <li><a href="/evportal/destination/product?catID=M87EPGE8Z">Reverse transcription kits</a></li> <li><a href="/evportal/destination/product?catID=M87F1J8UU">Real-time PCR supermixes</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf">troubleshooting reactions</a>.</p> <p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p> <p>&nbsp;</p> /webroot/web/html/lsr/products/SKUs 172-5275.html /webroot/web/html/lsr/products/SKUs/172-5275.html Product expiration (after shipping date) 2026 12 months 1 0 Storage at 4°C (after thawing) 2806 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725275&CartQuote=QT&quantity= false true false $3,167.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725275&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725275&quantity= 1725275
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Number Description Options
10031339 Instruction Manual, SsoAdvanced™ Universal SYBR® Green Supermix, Rev A Click to download
10000076382 SsoAdvanced™ Universal SYBR® Green Supermix Product Insert Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier, Ver A Click to download [ Add to Cart (Free) ]
6629 High-Throughput Real-Time PCR Solutions Brochure, Ver B Click to download
6773 Real-Time PCR Supermixes Flier, Ver A Click to download

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