SsoAdvanced™ PreAmp Supermix

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SsoAdvanced PreAmp Supermix

Overview

Optimal Unbiased Preamplification of up to 100 Targets

Number of qPCR Assays

Cq cycle quantification of targets in the preamp pool

SsoAdvanced™ PreAmp Supermix provides optimal unbiased preamplification of up to 100 SYBR® Green–based targets. A, preamplification of 100 PrimePCR™ Assays (SYBR® Green) with a ΔΔCq of <0.75. B, same data as A but showing range of Cq values (expression levels) of targets in the preamplification pool. Cq, quantification cycle.

Superior Unbiased Preamplification

Number of qPCR Assays

Cq cycle quantification of targets in the preamp pool

SsoAdvanced™ PreAmp Supermix provides superior unbiased preamplification of 24 probe-based qPCR assays compared to Life Technologies’ TaqMan PreAmp Master Mix.  A, preamplification of 24 PrimePCR™ probe-based cancer gene targets using SsoAdvanced PreAmp Supermix followed by qPCR using SsoAdvanced Universal Probes Supermix; average absolute ΔΔCq = 0.21 (1 of 24 >0.75). B, preamplification of 24 TaqMan probe-based cancer gene targets using TaqMan PreAmp Master Mix followed by qPCR using TaqMan Gene Expression Master Mix; average absolute ΔΔCq = 0.48 (6 of 24 > 0.75). Ideal ΔΔCq = 0.00. Cq, quantification cycle.

Optimal Preamplification of Low cDNA Input

Number of qPCR Assays

Cq cycle quantification of targets in the preamp pool

NEUROG2

SsoAdvanced™ PreAmp Supermix provides optimal preamplification of low cDNA input. Preamplification of a panel of 22 neuronal stem cell targets treated with retinoic acid to alter the stemness of NTERA2 cell lines with cDNA inputs of 10 ng, 1 ng, and 100 pg per preamplification reaction across 6 time points (days). A 1 µg no-preamplification control was used in the experiment. Results from NANOG (A, a high expressor), PAX6 (B, a medium expressor), and NEUROG2 (C, a low expressor) show consistent expression levels regardless of the cDNA input.

SsoAdvanced PreAmp Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for unbiased, target-specific preamplification of cDNA and genomic DNA. This PreAmp Supermix preamplifies DNA templates using up to 100 PrimePCR™ PreAmp SYBR® Green or probe-based qPCR assays, custom-designed SYBR® Green and probe-based assays (5’ nuclease), and TaqMan Assays.

  • Limited template DNA — start with as little as 100 pg of cDNA or genomic DNA from any precious or limited sample such as stem cells, laser capture microdissections, and formalin-fixed paraffin-embedded tissues (FFPE)
  • Versatile usability and applications — perform real-time or end-point PCR (SNP genotyping) on up to 100 SYBR® Green or probe-based targets with a simple, streamlined workflow
  • Compatible with various assaysPrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Assays

Preamplification of Targets without Bias Is Key

To obtain valid real-time PCR and end-point PCR data from limited samples, preamplification is often required. Some methods have been shown to introduce bias across the transcriptome/genome when comparing preamplified and nonpreamplified samples. The SsoAdvanced PreAmp Supermix is the first supermix that truly provides unbiased preamplification reactions regardless of the targets present in the reaction master mix, enabling more data to be obtained from a limited source.

Product Comparison

Product ΔΔCq* Chemistry Reactions Price**
SsoAdvanced PreAmp Supermix 90% within ± 0.75 SYBR® Green and probes 50 x 50 µl Least expensive
TaqMan PreAmp Master Mix 90% within ± 1.5 Probes only 40 x 50 µl Most expensive
PerfeCTa PreAmp 5x Supermix 90% within ± 1.5 SYBR® Green and probes 40 x 50 µl More expensive

* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.

Simple Workflow

Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.

Simple Workflow for SsoAdvanced PreAmp Supermix

Recommended RT-qPCR Reagents and Assays

Reverse Transcription

qPCR Supermixes

PreAmp and qPCR Assays

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 3 months
Customer guarantee (minimum usage time) at –20°C 12 months
SsoAdvanced PreAmp Supermix
SsoAdvanced™ PreAmp Supermix

172-5160
1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

List Price:   $625.00
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Number Description Options
6576 SsoAdvanced™ PreAmp Supermix and PrimePCR™ PreAmp Assays Flier, Rev A Click to download
10041826 SsoAdvanced PreAmp Supermix Product Insert, Rev A Click to download