Skirted 96-Well PCR Plates

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Skirted 96-Well PCR Plates

Overview

Hard-Shell® PCR Plate Compatibility Chart

Hard-Shell PCR plates represent an innovative concept in PCR plate design. The skirt and deck are molded from a rigid thermostable polymer. This hard shell prevents the distortion and shrinkage that may occur when regular single-component polypropylene PCR plates are exposed to the high temperatures of thermal cycling or heat sealing. Bio-Rad offers a variety of Hard-Shell 96-well and 384-well PCR plates.

Hard-Shell 96-Well PCR Plates

 
High-Profile
96-Well
Semi-Skirted Plates

Low-Profile
96-Well Skirted Plates

Low-Profile
96-Well
Semi-Skirted Plates

96-Well
480 Plates
Catalog number HSS-9xxx HSP-9xxx HSL-9xxx HSR-99xx
Well height High-profile Low-profile Low-profile Low-profile
Skirt Semi-skirted Full-skirted Semi-skirted Semi-skirted
ANSI/SBS standard dimensions Yes Yes Yes Yes
Thermal Cycler Compatibility
Bio-Rad® C1000™, C1000 Touch™, S1000™  
Bio-Rad® T100™      
Bio-Rad® DNA Engine®, DNA Engine Tetrad® and Tetrad 2, DNA Engine Dyad®, Dyad Disciple™    
Bio-Rad® iCycler®      
Applied Biosystems
0.2 ml tube cyclers
(2720, 9700, Veriti)
     
Applied Biosystems
0.1 ml tube cyclers
(9800 fast, Veriti fast)
     
Eppendorf Mastercycler series  
Real-Time PCR Detection System Compatibility
Bio-Rad® CFX Connect™, CFX96™, CFX96 Touch™    
Bio-Rad® CFX96 Touch Deep Well™    
Bio-Rad® iCycler iQ®, iQ™5, MyiQ™, MyiQ™2      
Bio-Rad® Chromo4™    
Bio-Rad® DNA Engine Opticon® and Opticon 2  
 
Applied Biosystems
standard
systems
(7300, 7500, 7900HT)
     
Applied Biosystems
fast systems
(7500 fast, ViiA 7 fast)
     
Roche LightCycler 480      
Eppendorf Mastercycler ep realplex  
Stratagene Mx series      
Other Instrument Compatibility
Applied Biosystems DNA sequencers
(3700, 3730, etc.)
     
Idaho Technology LightScanner      

Recommended   Compatible



Hard-Shell 384-Well PCR Plates

 
384-Well
Standard Plates

384-Well
480 Plates
Catalog number HSP-3xxx HSR-48xx
Well height Standard 384-well height Standard 384-well height
Skirt Full-skirted Full-skirted
ANSI/SBS standard dimensions Yes Yes
Thermal Cycler Compatibility
Bio-Rad C1000, C1000 Touch, S1000
Bio-Rad DNA Engine, DNA Engine Tetrad and Tetrad 2, DNA Engine Dyad, Dyad Disciple
Applied Biosystems 384-well cyclers
Eppendorf Mastercycler series
Real-Time PCR Detection System Compatibility
Bio-Rad® CFX384™, CFX384 Touch™
Applied Biosystems 384-well systems
(7900HT, ViiA 7)
Roche LightCycler 480  
Other Instrument Compatibility
Idaho Technology LightScanner  

Recommended   Compatible

High-Profile vs. Low-Profile

 

There are two common well heights for PCR tubes and plates:

  1. High-profile (full-height; around 20.7 mm) wells are “standard” and fit in most classical thermal cyclers and real-time PCR detection systems, such as Bio-Rad's iCycler®, iQ™ series real-time PCR detection systems, and Applied Biosystems' regular PCR systems and DNA sequencers.
  2. Low-profile (around 15.5 mm) wells are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR. They have been recommended for newer instruments such as Bio-Rad's C1000 Touch™ and S1000™ thermal cyclers, CFX series real-time PCR detection systems, and Applied Biosystems' fast PCR systems.

Instruments with adjustable lids can usually use both high-profile and low-profile wells. These instruments include Bio-Rad's 1000-series and DNA Engine® series thermal cyclers.

Amplification Reagents and Plastics Brochure
Download the Amplification Reagents and Plastics Brochure for more information on Bio-Rad products for gene expression analysis:
  • Product performance data
  • Quick and easy product selection tables
  • Ordering information

Use the links below to jump to particular guide sections:



    Cell Lysis


    RNA Isolation


    Reverse Transcription


    Real-Time PCR Reagents


    PCR Plastic Consumables


    PrimePCR™ Assays
    and Panels


    PCR Run and Data Analysis


Instrument Compatibility of PCR Plastic Consumables

Bio-Rad offers a large selection of thin-wall polypropylene PCR tubes and microplates precisely manufactured for optimal fit and cycling performance in Bio-Rad thermal cyclers and real-time systems. The variety of sealing methods, including caps, adhesive seals, and heat sealing films and foils, provides researchers with an array of sealing options to complement all thermal cycling workflows.


Thermal Cyclers
 
MJ Mini™ Gradient Thermal Cycler

T100™ Thermal Cycler

DNA Engine® Thermal Cycler Family

C1000 Touch™/S1000™ Thermal Cyclers
Tubes
0.5 ml individual tubes   96–deep well
reaction module
only
0.2 ml individual tubes
High-profile tube strips
Low-profile tube strips  
48- and 96-Well Plates
Hard-Shell® low-profile 96-well semi-skirted PCR plates      
Hard-Shell high-profile 96-well semi-skirted PCR plates  
Hard-Shell low-profile 96-well skirted PCR plates    
Multiplate™ high-profile unskirted PCR plates
Multiplate low-profile unskirted PCR plates  
iQ™ 96-well semi-skirted PCR plates  
   
Lids
Domed cap strips
Optical flat cap strips
Adhesive Seals
Microseal® 'A' film  
Microseal 'B' adhesive seals
 
Microseal 'C' optical seals  
Microseal 'F' foil  
Heat Sealer and Heat Sealing Films and Foils
PX1™ PCR plate sealer  
Optically clear heat seal  
Pierceable foil heat seal  
Peelable foil heat seal  


Real-Time PCR Detection Systems
  MiniOpticon System
MiniOpticon™ System
MyiQ2 and iCycler iQ Systems
iCycler iQ®, iQ™5,
MyiQ™, and MyiQ™2 Systems
Chromo4 System
Chromo4™ System
DNA Engine Opticon 2 System
DNA Engine Opticon® 2 System

CFX96 Touch™, CFX96 Touch Deep Well™, CFX384
Touch™
, and CFX Connect™ Systems
Tubes
High-profile individual tubes   CFX96 Touch
Deep Well only
Low-profile tube strips  
48- and 96-Well Plates
Hard-Shell
low-profile 96-well semi-skirted PCR plates
       
Hard-Shell high-profile 96-well semi-skirted PCR plates     CFX96 Touch
Deep Well only
Hard-Shell low-profile 96-well skirted PCR plates    
Multiplate
high-profile unskirted PCR plates
  CFX96 Touch
Deep Well only
Multiplate low-profile unskirted PCR plates  
iQ 96-well semi-skirted PCR plates      
384-Well Plates
Hard-Shell 384-well skirted PCR plates        
Lids and Adhesive Seals
Optical flat cap strips
Microseal 'B'
adhesive seals
 
Microseal 'C' optical seals  
Heat Sealer and Optically Clear Heat Seal
PX1 PCR plate sealer  
Optically clear heat seal  




Skirted PCR plates are the most stable type of microplate. A full skirt reduces plate distortion and ensures that the plate sits flat. These plates are ideal for automated handling and other robotic procedures. Skirted PCR plates are compatible with a smaller number of instruments than semi-skirted plates.

Our skirted PCR plates are compatible with Bio-Rad’s C1000 Touch™ and S1000™ Thermal Cyclers and all CFX series real-time PCR systems.

Use the PCR Plastics Consumables Selector Tool to determine individual plate compatibility with other instruments.

Several types of skirted PCR plates are available to suit different needs.

  • Hard-Shell® Low-Profile Skirted 96-Well PCR Plates, including Hard-Shell plates for the Roche LightCycler 480, have a patented* two-component design engineered to remain warp-free, permitting precise positioning for automation, and have black lettering for easy identification. Hard-Shell plates are compatible with heat, pressure, and adhesive sealing.
  • Concord™ Polycarbonate 96-Well PCR Plates are breathable plastic, so reaction volumes should be ≥ 20 µl. As they do not have raised rims, Chill-out® Liquid Wax is the best sealing option.
  • Microseal® Low-Profile Skirted 96-Well PCR Plates are single-component polypropylene plates with locator holes and flat vertical sidewalls for secure handling and easy bar coding. The footprint and well spacing meet ANSI/SBS microplate standards. Raised rims provide compatibility with most sealing systems and a tight seal. Barcoded, clear, white, black and colored plates are available.

Features and Benefits of Skirted PCR Plates

  • Skirted plates are low-profile permitting less reaction mix entering the gas phase, resulting in smaller changes in reaction conditions, as well as reduced condensate
  • Plates are free of DNase, RNase, and human DNA

Product Options

Skirted 96-Well PCR Plates

Reaction Volume

Available Colors

Barcoding

Hard-Shell Low-Profile 96-Well PCR Plates

5–125 µl
(200 µl maximum)

Clear, white, or black wells
Clear, white, black, red, blue, green, or yellow shells

Optional

Concord Polycarbonate 96-Well PCR Plates

20–150 µl
(210 µl maximum)

Clear

No

Microseal Low-Profile 96-Well PCR Plates

5–125 µl
(220 µl maximum)

Clear, white, black, pink, blue, or green

Optional

Related Products

The semi-automated PX1™ PCR Plate Sealer delivers reliable uniform sealing of a range of seal types on different styles of microplates. This programmable sealer minimizes sample evaporation and provides consistency.

Related Categories

Online Resources

PCR Plastics Selector

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear

HSP9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear

HSP9601B
8 packs of HSP9601 (50 plates per pack), a total of 400 plates, white shell/clear well PCR plate, rigid 2-component design

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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, red/clear

HSP9611
Pkg of 50, red shell/clear well PCR plate, rigid 2-component design

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$245.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, yellow/clear

HSP9621
Pkg of 50, yellow shell/clear well PCR plate, rigid 2-component design

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$245.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, blue/clear

HSP9631
Pkg of 50, blue shell/clear well PCR plate, rigid 2-component design

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$245.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, blue/white

HSP9635
Pkg of 50, blue shell/white well PCR plate, rigid 2-component design

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$265.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, green/clear

HSP9641
Pkg of 50, green shell/clear well PCR plate, rigid 2-component design

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$245.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, green/white

HSP9645
Pkg of 50, green shell/white well PCR plate, rigid 2-component design

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$265.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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$265.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, black/clear

HSP9661
Pkg of 50, black shell/clear well PCR plate for higher fluorescent signals, rigid 2-component design

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$245.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, black/white

HSP9665
Pkg of 50, black shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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$265.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, black/black

HSP9666
Pkg of 50, black shell/black well PCR plate, rigid 2-component design

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$265.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded

HSP9901
Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

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$255.00
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white, barcoded

HSP9955
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

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$270.00
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Hard-Shell® 96-Well PCR Plates, low profile, clear/clear, barcoded

HSR9901
Pkg of 25, clear shell/clear well PCR plate for Roche LightCycler 480 Real-Time PCR System, barcoded, rigid 2-component design

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$115.00
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Hard-Shell® 96-Well PCR Plates Kit, low profile, clear/clear, barcoded

HSR9901K
Kit for use with Roche LightCycler 480 Real-Time PCR System, includes 100 barcoded clear shell/clear well PCR plates (4 packs of HSR9901) and 100 Microseal® 'C' Optical Seals (MSC1001)

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$665.00
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Hard-Shell® 96-Well PCR Plates, low profile, clear/white, barcoded

HSR9905
Pkg of 25, clear shell/white well PCR plate for Roche LightCycler 480 Real-Time PCR System, barcoded, rigid 2-component design

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$115.00
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Hard-Shell® 96-Well PCR Plates Kit, low profile, clear/white, barcoded

HSR9905K
Kit for use with Roche LightCycler 480 Real-Time PCR System, includes 100 barcoded clear shell/white well PCR plates (4 packs of HSR9905) and 100 Microseal® 'C' Optical Seals (MSC1001)

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$665.00
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Microseal® 96-Well PCR Plates, low profile, skirted, clear

MSP9601
Pkg of 50, skirted PCR plate, one-component polypropylene, natural

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$245.00
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Microseal® 96-Well PCR Plates, low profile, skirted, clear, barcoded

MSP9605
Pkg of 50, skirted PCR plate, one-component polypropylene, barcoded, 8-digit sequential, Code 39, natural

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$255.00
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Microseal® 96-Well PCR Plates, low profile, skirted, pink

MSP9611
Pkg of 50, skirted PCR plate, one-component polypropylene, pink

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$255.00
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Microseal® 96-Well PCR Plates, low profile, skirted, blue

MSP9631
Pkg of 50, skirted PCR plate, one-component polypropylene, blue

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$255.00
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Microseal® 96-Well PCR Plates, low profile, skirted, green

MSP9641
Pkg of 50, skirted PCR plate, one-component polypropylene, green

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$255.00
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Microseal® 96-Well PCR Plates, low profile, skirted, white

MSP9651
Pkg of 50, skirted PCR plate, one-component polypropylene, white

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$255.00
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Microseal® 96-Well PCR Plates, low profile, skirted, black

MSP9661
Pkg of 50, skirted PCR plate, one-component polypropylene, black

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$255.00
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Concord™ 96-Well PCR Plates, polycarbonate, low profile, clear

CON9601
Pkg of 25, 96-well PCR plate, natural

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$130.00
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Number Description Options
06366 Instruction Manual, Hard-Shell 96-Well Thin-Wall PCR Plates, Rev C Click to download
10012853 Hard-Shell® 96-Well PCR Plates Product Insert, Rev E Click to download
6090 Amplification Reagents and Plastics Brochure, Rev E Click to download [ Add to Cart (Free) ]
10023997 Instruction Manual, PX1 PCR Plate Sealer, Rev A Click to download