Size Exclusion Chromatography Kit

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Size Exclusion Chromatography Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Hemoglobin/vitamin B12 sample mixture 1
Bio-Gel® P-60 columns with caps 8
Disposable plastic transfer pipets 10
Column chromatography buffer, 50 ml 1
Sample collection tubes, 5 ml 100
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Size Exclusion Flowchart
Size Exclusion Flowchart
Key Kit Features
  • Separate a mixture of biomolecules
  • Determine the number of components in a mixture
  • Study the chemical and physical properties of biomolecules
  • Apply the principles of chromatography
  • Complete in one 45 minute lab session
Curriculum Fit
Curriculum Fit - Size Exclusion Chromatography Kit
Molecules are separated by size
Series of 6 size exclusion chromatography columns showing the separation of Vitamin B12 and hemoglobin

Series of 6 size exclusion chromatography columns showing the separation of Vitamin B12 and hemoglobin.

PowerPoint Presentation for Workshops and for Teaching

Size Exclusion Chromatography Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Size Exclusion Chromatography Kit and includes instructions for using the kit.

Size Exclusion Chromatography Kit Download PowerPoint presentation
Size Exclusion Chromatography Kit

The Size Exclusion Chromatography Kit teaches the basic principles of size exclusion chromatography, a type of column chromatography used to separate solubilized molecules by their size or molecular weight.

Features and Benefits

With this kit, students are able to:

  • Separate a mixture of colored biomolecules
  • Determine the number of components in a mixture
  • Study the chemical and physical properties of biomolecules
  • Apply the principles of chromatography

How It Works

In this laboratory activity, a mixture of colored molecules in solution, hemoglobin (reddish-brown; molecular mass of 65,000 Da) and vitamin B12 (pink; molecular mass of 1,350 Da), is applied to a chromatography column containing microscopic porous beads. The contrasting colors are easily visible as the molecules pass through the column. The larger molecules bypass and flow around the beads. They are excluded from entering the pores by their size and flow through the column with relative speed. Smaller molecules enter the porous beads and are impeded in their flow through the column. The solutes' different rates of travel through the column lead to effective separation.

More Information

  • One 45 min laboratory activity
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Hemoglobin/vitamin B12 sample mixture 1
Bio-Gel® P-60 columns with caps 8
Disposable plastic transfer pipets 10
Column chromatography buffer, 50 ml 1
Sample collection tubes, 5 ml 100
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Size Exclusion Chromatography Kit

166-0008EDU
Basic column-chromatography classroom study kit, includes Bio-Gel P-60 columns, sample mixture, disposable pipets, buffer, collection tubes, curriculum, for 32 students; education use only

List Price:   $102.50
EDU Price:   $82.00   What is this?
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Number Description Options
3056 Size Exclusion Chromatography Kit, Biotechnology Explorer, Quick Guide Click to download
4006103 Instruction Manual, Size Exclusion Chromatography Kit, Biotechnology Explorer, Rev C Please login to download this document [ Add to Cart (Free) ]