Silver Stains

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Overview

Specifications
Product Time Number of gels Shelf life Storage Lowest Sensitivity
Silver Stain 2 hours 48 mini gels 1 year Ambient 0.6 – 1.2 ng
Silver Stain Plus 1.5 hours 40 mini gels 1 year 4°C 0.6 – 1.2 ng
Dodeca Silver Stain 3 hours 12 large format gels 1 year   0.25 – 0.5 ng
Reproducibility of Dodeca Silver Stain

A set of 12 large format gels was stained with the Dodeca silver stain kit in the large Dodeca stainer. The gels contained mouse liver samples (80 µg) prepared with the ReadyPrep protein extraction (soluble/insoluble) kit.

The samples were cup loaded on 24 cm ReadyStrip IPG strips (pH 5–8) and run with the PROTEAN IEF cell at 110,000 V-hr, then applied to 12% Tris-HCl PROTEAN Plus precast gels and run in the second dimension in the PROTEAN Plus Dodeca cell at ~2,900 V-hr. One-third of a Precision Plus Protein unstained standard plug was applied to the right side of each gel for molecular weight determination.

Silver Stain Results

SDS-PAGE stained with A, Coomassie Brilliant Blue R-250, and B, Bio-Rad's silver stain. Samples were run on 12% polyacryl-amide gels 0.75 mm thick. Lane 1, bovine brain homogenate soluble fraction, 20 µI; lane 2, bovine brain homogenate soluble fraction, 10 µI; lane 3, Bio-Rad's SDS-PAGE low molecular weight standards, 1/40 dilution; lane 4, Bio-Rad's SDS-PAGE standards, 1/80; lane 5, Bio-Rad's SDS-PAGE standards, 1/160.

Silver Stain

Gel stained with Dodeca silver stain. The sample was mouse liver run on a 12% Tris-HCl PROTEAN Plus precast gel.

Silver staining is a highly sensitive method for detecting proteins and nucleic acids in polyacrylamide slab gels. Bio-Rad silver stain kits are 10–50-fold more sensitive than Coomassie Brilliant Blue R–250 for proteins (detection is ~0.1 ng/mm2) and 2–5-fold more sensitive than ethidium bromide for single- and double-stranded DNA and RNA. Bio-Rad offers three types of silver stain kits, each having unique features for your experimental needs.

Bio-Rad silver stain kit — This original silver stain kit, derived from the method of Merril et al. (1981), is ideal for staining polysaccharides, lipoproteins, nucleic acids, and highly glycosylated proteins.

Silver Stain Plus kit — Silver Stain Plus stain is the most sensitive and easiest-to-use silver stain. The Silver Stain Plus kit is derived from the method developed by Gottlieb and Chavko (1987). The chemistry has been modified for both protein and nucleic acid staining in polyacrylamide and agarose gels. The Silver Stain Plus Kit can detect as little as 0.6 ng of protein and DNA and requires only three steps, allowing visualization of your sample is less than 2 hours.

Dodeca silver stain kits — Dodeca silver stain kits are designed especially for use with Dodeca stainers. The kits are available in two sizes, designed to fill the small or large Dodeca stainer to maximum capacity. Both kits have been optimized to deliver high-sensitivity, convenient, and reproducible staining of large numbers of gels. The kits are based on the method described by Sinha et al. (2001), in which protein-bound silver ions are chemically reduced to form visible metallic silver. The Dodeca silver stain kits detect nanogram levels of proteins in polyacrylamide gels in less than 3 hours.

References

Gottlieb M and Chavko M (1987). Silver staining of native and denatured eukaryotic DNA in agarose gels. Anal Biochem 165, 33–37.

Merril CR et al. (1981). Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211, 1437–1438.

Sinha P et al. (2001). A new silver staining apparatus and procedure for matrix-assisted laser desorption/ionization-time of flight analysis of proteins after two-dimensional electrophoresis. Proteomics 1, 835–840.

Dodeca Silver Stain Kit

161-0480
Kit for silver staining polyacrylamide gels with the large Dodeca stainer (165-3400), for 12 large gels

List Price:   $696.00
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Hazardous  
Dodeca Silver Stain Kit

161-0481
Kit for silver staining polyacrylamide gels with the small Dodeca stainer (165-3401), for 12 large or 24 midi gels

List Price:   $497.00
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Image Developer Concentrate

161-0472
2.5 ml, replacement component of Dodeca silver stain kits

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Sensitizer Concentrate

161-0473
700 ml, replacement component of Dodeca silver stain kits

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Sensitizer Concentrate

161-0474
1 L, replacement component of Dodeca silver stain kits

List Price:   $220.00
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Background Reducer Concentrate

161-0475
75 ml, replacement component of Dodeca silver stain kits

List Price:   $68.00
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Background Reducer Concentrate

161-0476
125 ml, replacement component of Dodeca silver stain kits

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Silver Reagent Concentrate

161-0477
140 ml, replacement component of Dodeca silver stain kits

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Silver Reagent Concentrate

161-0478
200 ml, replacement component of Dodeca silver stain kits

List Price:   $170.00
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Development Buffer Concentrate

161-0479
700 ml, replacement component of Dodeca silver stain kits

List Price:   $138.00
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Development Buffer Concentrate

161-0482
1 L, replacement component of Dodeca silver stain kits

List Price:   $194.00
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Silver Stain Plus Kit

161-0449
Kit for detection of nanogram quatities of DNA and protein in polyacrylamide and agarose gels, for 13 full-size or 40 mini gels

List Price:   $205.00
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Development Accelerator Reagent

161-0448
50 g, replacement component of the Silver Stain Plus kit (#161-0449)

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Fixative Enhancer Concentrate

161-0461
1 L, replacement component of the Silver Stain Plus kit (#161-0449)

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Silver Complex Solution

161-0462
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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Reduction Moderator Solution

161-0463
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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Image Development Reagent

161-0464
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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Silver Stain Kit

161-0443
Polysaccharides and glycosylated proteins staining kit, includes oxidizer and silver reagent concentrates, developer, for 24 full-size or 48 mini gels

List Price:   $180.00
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Silver Stain Developer

161-0450
115 g, replacement component of the silver stain kit (#161-0443)

List Price:   $22.00
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Silver Stain Developer

161-0447
4 x 115 g, replacement component of the silver stain kit (#161-0443)

List Price:   $90.00
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Oxidizer Concentrate

161-0444
480 ml, replacement component of the silver stain kit (#161-0443)

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Hazardous  
Silver Reagent Concentrate

161-0445
480 ml, replacement component of the silver stain kit (#161-0443)

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Number Description Options
2971 Dodeca Silver Stain Kit Product Information Sheet, Rev B Click to download
1089 The Bio-Rad Silver Stain, Rev E Click to download
LIT442 Instruction Manual, Silver Stain Plus, Rev E Click to download
4110150 Instruction Manual, Dodeca Silver Stain Kit, Rev B Click to download
LIT34 Instruction Manual, Bio-Rad Silver Stain Click to download