Trans-Blot® Turbo™ Transfer System

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Overview

Superior Transfer Efficiency

Higher sensitivity and better transfer efficiency is seen with the Trans-Blot® Turbo™ system in comparison to other blotting techniques. This data set demonstrates the successful transfer of the 1.25 ng protein band only with the Trans-Blot Turbo system. Serial dilutions of transferrin were separated on a Criterion™ TGX™ 4–20% gel and transferred using four different blotting techniques. All images are adjusted to the same settings.




Trans-Blot® Turbo™ System Tour
Trans-Blot® Turbo™ System Instructional Video


Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.

Trans-Blot® Turbo™ System vs. Traditional Blotting Techniques


See how the Trans-Blot Turbo system measures up against traditional blotting techniques.

Trans-Blot® Turbo™ System vs. iBlot


See how the Trans-Blot Turbo system performs compared to the iBlot dry blotting system.

Transfer in 3, 7, or 10 Min

Turbo Transfers with Trans-Blot® Turbo™ Transfer Packs

  • 3-minute protocol — a single Mini-PROTEAN® TGX™ gel (for proteins with MW 5–150 kD) can be transferred in as little as 3 min
  • 7-minute protocol — up to 4 mini or 2 midi gels with mixed-molecular weight proteins (MW 5–150 kD) can be efficiently transferred in 7 min
  • 10-minute protocol — up to 4 mini or 2 midi gels with high-molecular weight proteins (MW 25–300+ kD) can be efficiently transferred in 10 min

E. coli lysate (6 µg) was diluted two-fold. Samples were separated with Mini-PROTEAN TGX gels, transferred with the Trans-Blot Turbo system, stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.


Better Transfer of Different Types of Proteins

2x Protein Transfer

Quantitation performed on equivalent 2-D gels transferred with the Trans-Blot® Turbo™ and the iBlot systems demonstrated twice the number of proteins transferred and detected with the Trans-Blot Turbo system. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. Duplicate gels were transferred with the Trans-Blot Turbo and iBlot systems, both using manufacturers’ recommended 7 min protocol. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system.


Blotter Comparison
 
  Tank Semi-dry iBlot Trans-Blot® Turbo™ system
Speed of transfer •• ••• ••••
Flexibility •• •• •••
Transfer efficiency even for high MW ••• •• •••
Throughput 2 mini gels 6 mini/3 midi gels 2 mini/1 midi gel 4 mini/2 midi gels
Power requirement External power supply (PowerPac™ HC) External power supply (PowerPac™ HC) Integrated power supply Integrated power supply
User interface None None Preset protocols only Preset protocols and easy customization

The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. The system enables blot transfer of protein in as little as 3 minutes without sacrificing performance when compared with traditional tank protein blotting. The Trans-Blot Turbo Transfer Packs include an optimized buffer, membrane, and filter paper combination that provides superior blot transfers when paired with the Trans-Blot Turbo blotting apparatus. Protein blotting consumables are available in a ready-to-use, pre-packaged stack or in bulk quantities that require assembly.

Features and Benefits of the Trans-Blot Turbo Transfer System

  • Rapid blot transfer 3 min transfers of mini or midi gels
  • High throughput — can transfer 1–4 mini or 1–2 midi gels in a single run
  • Greater transfer efficiencyhigher transfer efficiency than other protein blotting methods
  • Flexible design — allows the user to customize transfer conditions and is compatible with traditional semi-dry consumables
  • Environmentally friendly — environmentally safe consumables eliminate disposal cost

Watch this short video to learn more about the Trans-Blot Turbo system.

Applications and Uses of the Trans-Blot Turbo Transfer System

This protein blotting system is a powerful tool in the western blotting workflow, enabling the visualization and identification of proteins for downstream analysis. The Trans-Blot Turbo transfer system can be used in the following applications:

More Information

Trans-Blot Turbo transfer packs come with either PVDF or nitrocellulose membranes:

Select the Trans-Blot Turbo RTA transfer kit for a low-fluorescence membrane option.

Use Bio-Rad’s Precision Plus Protein™ WesternC™ standards and Clarity™ Western ECL substrate for chemiluminescence detection on both film and digital imagers. See how the Trans-Blot Turbo transfer system fits into the V3 Western Workflow™ to streamline your western blotting process.

See the ordering information for Mini-PROTEAN® TGX™ precast gels and Criterion™ TGX™ precast gels to select the best gel for your application.

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Trans-Blot® Turbo™ Transfer Starter System

1704155
Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit

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$3,085.00
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Trans-Blot® Turbo™ Mini PVDF Transfer Packs

1704156
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system

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$170.00
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Trans-Blot® Turbo™ Midi PVDF Transfer Packs

1704157
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system

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$200.00
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Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs

1704158
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system

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$150.00
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Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs

1704159
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system

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$180.00
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Trans-Blot Turbo RTA Mini Nitrocellulose Transfer Kit
Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit, for 40 blots

1704270
Ready-to-assemble transfer kit includes 40 mini-sized nitrocellulose membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

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$275.00
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Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit
Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit, for 40 blots

1704271
Ready-to-assemble transfer kit includes 40 midi-sized nitrocellulose membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

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$360.00
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Trans-Blot Turbo RTA Mini PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit, for 40 blots

1704272
Ready-to-assemble transfer kit includes 40 mini-sized PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

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$295.00
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Trans-Blot Turbo RTA Midi PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit, for 40 blots

1704273
Ready-to-assemble transfer kit includes 40 midi-sized PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

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$400.00
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Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Mini LF PVDF Transfer Kit, for 40 blots

1704274
Ready-to-assemble transfer kit includes 40 mini-sized LF PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

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$295.00
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Trans-Blot Turbo RTA Midi LF PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Midi LF PVDF Transfer Kit, for 40 blots

1704275
Ready-to-assemble transfer kit includes 40 midi-sized LF PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

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$400.00
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Trans-Blot® Turbo™ Cassette

1704151
Pkg of 1, cassette, to hold 1 midi or up to 2 mini blotting sandwiches, for use with Trans-Blot Turbo transfer system

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$725.00
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Trans-Blot® Turbo™ Base

1704152
Blotting instrument base, does not include any cassettes

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$1,625.00
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Number Description Options
10039859 Trans-Blot Turbo Instrument Protocol, Rev A Click to download
10020688 Instruction Manual, Trans-Blot® Turbo™ Blotting System, Rev F Click to download
10016505 Trans-Blot® Turbo™ Transfer System Quick Start Guide, Rev D Click to download [ Add to Cart (Free) ]
10019593 Trans-Blot® Turbo™ Transfer System Transfer Pack Quick Start Guide, Rev E Click to download [ Add to Cart (Free) ]
10026778 Trans-Blot Turbo Transfer System RTA Transfer Kits Quick Start Guide, Rev D Click to download
6487 Transferring High Molecular Weight Proteins on the Trans-Blot Turbo Transfer System Quick Start Guide, Rev A Click to download [ Add to Cart (Free) ]
6039 Trans-Blot® Turbo™ Transfer System Brochure, Rev E Click to download [ Add to Cart (Free) ]
5535 Mini-PROTEAN® Electrophoresis System Brochure, Rev F Click to download [ Add to Cart (Free) ]
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
6088 V3 Western Workflow with Gel Doc EZ Imager Brochure, Rev B Click to download [ Add to Cart (Free) ]
RP0051 Stain-Free Approach for Western Blotting Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download [ Add to Cart (Free) ]
6147 Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B Click to download [ Add to Cart (Free) ]
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide, Rev B Click to download [ Add to Cart (Free) ]