Science of Opioid Dependence Kit

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Science of Opioid Dependence Kit


The Science of Opioid Dependence Kit puts your students in the roles of researcher and doctor as they explore genetic and environmental links to opioid dependence. In this kit, your students will design a human genetic research study; they will conduct experiments using DNA gel electrophoresis to determine whether a mutation in a human dopamine receptor gene influences opioid dependence.

Features and Benefits

  • Curriculum connections to genetics, neurobiology, DNA structure, PCR, and statistical analysis
  • Alignment with AP Biology Big Idea 3
  • Five activities with optional timelines and branch points
  • Reagents and consumables for 8 lab stations (32 students)

Students will

  • Explore the links between opioid dependence, genetics, the neurobiology of reward pathways, and environment factors
  • Design a human genetic research study and experiments
  • Analyze pre-amplified patient PCR samples by DNA gel electrophoresis
  • Review class and published allele frequency data with optional chi-squared analysis

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