Sample Loading Buffers and Reagents

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Overview

Premixed Sample Buffer Composition
Buffer Formulation Applications
4x Laemmli sample buffer 277.8 mM Tris-HCI, pH 6.8, 4.4% LDS, 44.4% (v/v) glycerol,
0.02% bromophenol blue
SDS-PAGE
2x Laemmli sample buffer 65.8 mM Tris-HCI, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol,
0.01% bromophenol blue
SDS-PAGE
Native sample buffer 62.5 mM Tris-HCI, pH 6.8, 40% (v/v) glycerol,
0.01% bromophenol blue
Native PAGE
Tricine sample buffer 204 mM Tris-HCI, pH 6.8, 2% SDS, 40.8% (v/v) glycerol,
0.04% Coomassie Brilliant Blue G-250
Peptide and small protein SDS-PAGE
TBE-urea sample buffer 89 mM Tris-HCI, pH 8.3, 89 mM boric acid, 2 mM EDTA,
7 M urea, 12% Ficoll, 0.01% bromophenol blue,
0.02% xylene cyanole FF
Denaturing ssDNA, RNA
IEF sample buffer 50% (v/v) glycerol IEF
Zymogram sample buffer 65.8 mM Tris-HCI, pH 6.8, 26.3% (w/v) glycerol, 4.2% SDS, 0.01% bromophenol blue Protease analysis
5X Nucleic acid sample buffer 50 mM Tris-HCI, pH 8.0, 25% (w/v) glycerol, 5 mM EDTA,
0.2% bromophenol blue, 0.2% xylene cyanole FF
Nondenaturing dsDNA, TBE gels

Sample Loading Buffers

Premixed loading buffers remove variables that cause lane-to-lane running anomalies. No preparation is required, saving valuable time. Bio-Rad premixed sample buffers are available for numerous applications, including native PAGE, SDS-PAGE, peptide analysis, analytical IEF, nucleic acid sample preparation (denaturing and nondenaturing), and zymogram gel sample preparation.

Bio-Rad electrophoresis-purity reagents are quality controlled to ensure reproducible results and are available as individual reagents or as premixed buffers. For more information, request bulletin 2317.

Premixed Electrophoresis Buffers

Simplify and standardize your sample loading with premixed sample buffers. The concentrated formulas allow these buffers to be used with both liquid and lyophilized samples. All premixed sample buffers are tested to ensure quality and consistency.

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2x Laemmli Sample Buffer

1610737
30 ml, premixed protein sample buffer for SDS-PAGE

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$14.00
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Native Sample Buffer

1610738
30 ml, premixed protein sample buffer for native PAGE

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$16.00
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Tricine Sample Buffer

1610739
30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE

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$14.00
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4x Laemmli Sample Buffer

1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE

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$15.00
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IEF Sample Buffer

1610763
30 ml, protein sample buffer, 50% glycerol, for use in IEF with both liquid and lyophilized samples

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$14.00
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Zymogram Sample Buffer

1610764
30 ml, protein sample buffer, 62.5 mM Tris-HCl, pH 6.8, 25% glycerol, 4% SDS, 0.01% bromophenol blue, for use in protease analysis

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$16.00
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XT Sample Buffer

1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels

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$13.00
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XT Reducing Agent

1610792
1 ml, 20x reducing agent for use with all Criterion XT gels

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$23.00
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Dithiothreitol (DTT)

1610610
1 g, dithiothreitol (DTT) reducing agent

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$27.00
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Dithiothreitol (DTT)

1610611
5 g, dithiothreitol (DTT) reducing agent

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$79.00
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2-Mercaptoethanol

1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

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$23.00
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Tributylphosphine (TBP)

1632101
0.6 ml, 200 mM, reducing agent for use in protein sample preparation applications

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$43.00
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EDTA

1610729
500 g, ≥99% pure EDTA (disodium ethylenediaminetetraacetate dihydrate) powder

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$70.00
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Iodoacetamide

1632109
30 g, alkylating agent for use in protein sample preparation applications

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$140.00
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Number Description Options
4006027 Instruction Manual, Native Sample Buffer, Rev F Click to download
4106111 Instruction Manual, Zymogram Sample Buffer, Rev B Click to download
4106115 Instruction Manual, TBE-Urea Sample Buffer, Rev B Click to download
4006030 Instruction Manual, Tricine Sample Buffer, Rev E Click to download
4006028 Instruction Manual, Laemmli Sample Buffer, Rev J Click to download
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]