S3e™ Cell Sorter

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S3e Cell Sorter

Overview

Automated Drop Delay Measurement and Monitoring

Optimal Cell Sorting Performance
and Purity

Obtaining optimal sort purity and maximizing recovery requires accurate drop delay determination, or measurement of the distance between the laser point of analysis and the last connected drop.

Historically, the drop delay process has been labor intensive and has required bead counting on a microscope or image processing with a camera.

ProDrop™ Technology automates drop delay determination and accurately calculates drop delay values, resulting in less opportunity for error and leading to better recovery. The exact drop delay value is calculated by measuring the number of ProLine™ Calibration Beads in the waste stream during the QC process.

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S3e™ Biosafety System Class I

Easy access to the S3™ and S3e Cell Sorters:

 

Easy access to the S3 cell sorter

The S3e Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier and accessible to both novices and experts.

This compact system is equipped with either one or two lasers and up to four fluorescence detectors plus forward- and side-scatter detectors.

Features and Benefits of the S3e Cell Sorter

  • Hands-free nozzle tip alignment — features the fully automatic AutoGimbal™ System, driven completely by software
  • Simplified instrument setup — fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 1–4 color sorting with minimal training
  • Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system
  • 2-way cell sorting — sort two different defined populations at the same time
  • High speed and high purity — sort cells fast while maintaining high sensitivity and purity
  • Intuitive interface — user-friendly ProSort™ Software enables effortless instrument control and sort logic definition

Among the many innovations of the S3e Cell Sorter, two key state-of-the-art advancements are ProDrop™ Technology and the AutoGimbal System:

ProDrop Technology produces extremely accurate and precise results by:

  • Automating drop delay calculation and droplet break-off monitoring
  • Allowing drop delay calculation through direct counting of bead events
  • Simplifying one of the most complex and error-prone aspects of cell sorting

The highly sophisticated AutoGimbal System automates alignment of the nozzle tip and stream to optics by:

  • Providing hands-free alignment at all points on the stream and consistency in stream position from day to day and user to user
  • Combining picomotor fine motion positioning, imaging, and response to software algorithms using camera images

The affordable S3e Cell Sorter provides exceptional sort purity, recovery, and yield without compromising performance and sensitivity.

The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry, or for cells labeled with fluorescent markers such as antibodies labeled with PE, PE tandems, and other fluorophores excited by the 488, 561, and 640 nm lasers.

More Information

Custom-designed to work specifically with the S3™ and S3e Cell Sorters, the optional S3e™ Biosafety System Class I (145-1078) provides protection for both the user and the environment from aerosols created while sorting.

The biosafety system is an affordable alternative to the large and expensive traditional biosafety hoods offered for cell sorters.

Bio-Rad now offers antibodies for flow cytometry through AbDSerotec.com

AbD Serotec®
A Bio-Rad Company

Thousands of antibodies tested for flow cytometry are available from AbD Serotec, a Bio-Rad company.

Find flow cytometry antibodies for use with the S3e Cell Sorter and other platforms by searching AbD Serotec's database for parameters such as target species, host, isotype, or clone. A number of associated isotype controls, kits, reagents, and additional resources for flow cytometry are also available from AbDSerotec.com.

Order primary antibodies for flow cytometry online or contact your Bio-Rad or AbD Serotec sales representative for more information.

See All Available Antibodies for Flow Cytometry

 
Droplet frequency 37–43 kHz
Sorting type True jet-in-air for high-performance sorting
Sorting rate No hardware limitations for sort rate, limited only by droplet frequency and application
Sorting purity >99% pure
Nozzle size 100 µm
Sorting direction 2-way sorting
Sorting collection Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Lasers Single laser: 488 nm 100 mW
Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
Dual laser: 488 and 561 or 640 nW 100 mW
Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
Sensitivity <125 MESF for FITC and PE
Temperature range Sample and collection temperature control system: 4–37°C
Peltier solid state system
Fluidics Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format FCS 3.1
Dimensions (W x D x H) 70 x 65 x 65 cm (2.3 x 2.1 x 2.1 ft)
S3e™ Cell Sorter (488 nm)

145-1005
S3e Cell Sorter, 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Cell Sorter (488/561 nm)

145-1006
S3e Cell Sorter, 488 and 561 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Cell Sorter (488/640 nm)

145-1008
S3e Cell Sorter, 488 and 640 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

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S3e™ Biosafety System Class I

145-1078
Biosafety System Class I for S3™ and S3e Cell Sorters, includes HEPA filter, fan blower, base, aluminum frame, 30 mil vinyl panels with magnetic attachment

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S3e™ Cell Sorter (488 nm) with S3e Biosafety System Class I

145-1029
S3e Cell Sorter, 488 nm 100 mW laser, 2 fluorescence detectors, with S3e Biosafety System Class I

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S3e™ Cell Sorter (488/561 nm) with S3e Biosafety System Class I

145-1030
S3e Cell Sorter, 488 nm and 561 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

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S3e™ Cell Sorter (488/640 nm) with S3e Biosafety System Class I

145-1032
S3e Cell Sorter, 488 nm and 640 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

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Number Description Options
6614 S3e™ Cell Sorter Brochure, Rev B Click to download
6329 S3 Cell Sorter Flier, Rev B Click to download
6325 S3 Cell Sorter Brochure, Rev D Click to download [ Add to Cart (Free) ]
10032007 S3™ and S3e™ Cell Sorters Quick Guide, Rev D Click to download
10031105 Instruction Manual, S3™ and S3e™ Cell Sorters, Rev D Click to download
6404 S3™ Biosafety System Class I Flier, Rev A Click to download
6503 S3e™ Biosafety System Class I Brochure, Rev B Click to download
10030825 ProFlow™ Sort Grade 8x Sheath Fluid Product Insert, Rev B Click to download
10032248 ProFlow™ Sort Grade Water Product Insert, Rev A Click to download
10033040 ProLine™ Rainbow Beads Product Insert, Rev A Click to download
10041517 ProLine Universal Calibration Beads Product Insert, Rev A Click to download
10033232 Instruction Manual, S3™ Biosafety System Class I, Rev B Click to download
10041735 S3™ Filter Block (A or B) with Dichroic Filter Holders Product Insert, Rev A Click to download
10041736 S3™ Individual Filter Holder Product Insert, Rev A Click to download
10041737 S3™ Individual Dichroic Filter Holder Product Insert, Rev A Click to download
6609 Isolation of Stably Transfected Fluorescent Cells with the S3™ Cell Sorter Application Note, Rev A Click to download [ Add to Cart (Free) ]
6681 Discriminating Copy Number Variation from Heterogeneous Samples Using the S3™ Cell Sorter and the QX200™ Droplet Digital™ PCR System Application Note, Rev A Click to download
6697 S3e™ Cell Sorter Flier, Rev A Click to download