Running Buffers and Reagents

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Reduce preparation time and eliminate a source of error by using Bio-Rad’s range of premixed protein electrophoresis running buffers. There are no reagents to weigh or filter; just dilute with distilled deionized water. A wide variety of premixed Tris buffers (such as Tris/glycine/SDS, Tris/glycine, and Tris/tricine/SDS) and high-purity, electrophoresis-grade powders (such as Tris, glycine, tricine, and urea) are available for protein electrophoresis and can be used with both handcast gels and Bio-Rad’s precast gels (see the protein gel selection guide).

Features and Benefits of Running Buffers and Reagents for Protein Electrophoresis

  • Premixed buffers – all electrophoresis-purity reagents used are quality controlled for reproducible results; save preparation time while avoiding potential error due to incorrect buffer concentration
  • Variety of sizes – electrophoresis running buffers and reagents are available in packages ranging from 250 ml bottles to 5 L cubes for different throughput needs
  • Bulk 5 L boxes offer economy and convenience; the boxes feature easy-pouring spouts and are compact and stackable to save bench space

Applications and Uses of Running Buffers and Reagents for Protein Electrophoresis

Bio-Rad’s high-quality premixed electrophoresis running buffers are used for

More Information

Compare protein electrophoresis buffers to select the most appropriate buffers for your workflow.

To choose the best gel for your application, see the gel selection guide. Choices include the innovative TGX™ SDS-PAGE system with precast Mini-Protean® TGX™ and Mini-Protean® TGX Stain-Free™ Gels, and midi-sized Criterion™ TGX™ and Criterion™ TGX Stain-Free™ Gels. For hand casting, TGX™ FastCast™ and TGX Stain-Free™ FastCast™ Acrylamide Solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels.

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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10x Tris/Glycine/SDS
10x Tris/Glycine/SDS

1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$28.00
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10x Tris/Glycine/SDS

1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$96.00
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10x Tris/Glycine Buffer for Western Blots and Native Gels
10x Tris/Glycine Buffer for Western Blots and Native Gels

1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$28.00
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10x Tris/Glycine Buffer for Western Blots and Native Gels
10x Tris/Glycine Buffer for Western Blots and Native Gels

1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$96.00
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10x Tris/Tricine/SDS Running Buffer, 1 L

1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$88.00
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10x IEF Anode Buffer, 250 ml

1610761
Pkg of 1, 250 ml, electrophoresis buffer, 7 mM phosphoric acid, for use in isoelectric focusing

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$11.00
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10x IEF Cathode Buffer, 250 ml
10x IEF Cathode Buffer, 250 ml

1610762
Pkg of 1, 250 ml, electrophoresis buffer, 20 mM lysine, 20 mM arginine, for use in isoelectric focusing

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$58.00
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10x Zymogram Renaturation Buffer, 125 ml
10x Zymogram Renaturation Buffer, 125 ml

1610765
Pkg of 1, 125 ml, 10x premixed electrophoresis buffer, contains 25% Triton X-100, for use with Zymogram Gels

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$13.00
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10x Zymogram Development Buffer, 125 ml
10x Zymogram Development Buffer, 125 ml

1610766
Pkg of 1, 10x buffer, 1x final concentration contains 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM CaCl2, 0.02% Brij-35, for use with Zymogram Gels

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$14.00
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XT MOPS Running Buffer
XT MOPS Running Buffer

1610788
Pkg of 1, 500 ml, electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels

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$64.00
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XT MES Running Buffer

1610789
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels

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$64.00
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XT Tricine Running Buffer

1610790
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Tris-Acetate Gels

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$64.00
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20x XT MOPS Running Buffer Kit
20x XT MOPS Running Buffer Kit

1610793
Pkg of 1, electrophoresis buffer kit, includes 500 ml XT MOPS Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml XT Reducing Agent

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$98.00
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XT MES Buffer Kit

1610796
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT MES Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent

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$98.00
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XT Tricine Buffer Kit

1610797
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent

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$98.00
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Glycine, 250 g
Glycine, 250 g

1610717
Pkg of 1, 250 g, glycine (aminoacetic acid) powder

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$24.00
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Glycine, 1 kg
Glycine, 1 kg

1610718
Pkg of 1, 1 kg, glycine (aminoacetic acid) powder

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$54.00
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Glycine, 2 kg
Glycine, 2 kg

1610724
Pkg of 1, 2 kg, glycine (aminoacetic acid) powder

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$115.00
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Tricine

1610713
Pkg of 1, 500 g, Tricine (N-Tris[hydroxymethyl]methylglycine) powder

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$220.00
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Tris

1610716
Pkg of 1, 500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

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$33.00
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Tris

1610719
Pkg of 1, 1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

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Urea

1610730
Pkg of 1, 250 g, urea (carbamide)

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$27.00
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Urea

1610731
Pkg of 1, 1 kg, urea (carbamide)

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$78.00
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2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download