Running Buffers and Reagents

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Overview

Protein Electrophoresis Buffers
Buffer 1 x Formulation Applications
10x Tris/glycine/SDS 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 General SDS-PAGE
10x Tris/glycine 25mM Tris, 192 mM glycine, pH 8.3 Native PAGE
10x Tris/Tricine/SDS 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 Peptide SDS-PAGE
10x IEF anode buffer 7 mM phosphoric acid Analytical isoelectric focusing
10x IEF cathode buffer 20 mM lysine, 20 mM arginine Analytical isoelectric focusing
10x zymogram renaturation buffer 2.5% Triton X-100 Protease analysis; renatures enzymes after electrophoresis
10x zymogram development buffer 50mM Tris-HCI, pH 7.5, 200 mM naCl, 5 mM CaCl2, 0.02% Brij 35 Protease analysis; activates enzymes after elecrophoresis
Nucleic Acid Electrophoresis Buffers
10x TBE 89 mM Tris, 89 mM boric acid 2 mM EDTA, pH 8.3 Nucleic acid electrophoresis/sequencing; polyacrylamide or agaose gels
10x TBE extended range 130 mM Tris, 45 mM boric acid, 2.5 mM EDTA Nucleic acid electrophoresis/sequencing; polyacrylamide or agaose gels; extends the buffer capacity for longer DNA sequencing runs
50x TAE 40mM Tris, 20 mM acetic acid, 1 mM EDA pH 8.0 Nucleic acid electrophoresis/sequencing; polyacrylamide or agaose gels

Reduce preparation time and eliminate a source of error by using Bio-Rad’s range of premixed protein electrophoresis running buffers. There are no reagents to weigh or filter; just dilute with distilled deionized water. A wide variety of premixed Tris buffers (such as Tris/glycine/SDS, Tris/glycine, and Tris/tricine/SDS) and high-purity, electrophoresis-grade powders (such as Tris, glycine, tricine, and urea) are available for protein electrophoresis and can be used with both handcast gels and precast gels.

Features and Benefits of Running Buffers and Reagents for Protein Electrophoresis

  • Premixed buffers – all electrophoresis-purity reagents used are quality controlled for reproducible results; save preparation time while avoiding potential error due to incorrect buffer concentration
  • Variety of sizes – electrophoresis running buffers and reagents are available in packages ranging from 250 ml bottles to 5 L cubes for different throughput needs
  • Bulk 5 L boxes offer economy and convenience; the boxes feature easy-pouring spouts and are compact and stackable to save bench space

Applications and Uses of Running Buffers and Reagents for Protein Electrophoresis

Bio-Rad’s high-quality premixed electrophoresis running buffers are used for

More Information

Compare protein electrophoresis buffers to select the most appropriate buffers for your workflow.

To select the best gel for your application, see the gel ordering guide for the Mini-PROTEAN® TGX™ precast gels and the gel ordering guide for the Criterion™ TGX precast gels.

10x Tris/Glycine/SDS
10x Tris/Glycine/SDS

161-0732
1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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10x Tris/Glycine/SDS

161-0772
5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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10x Tris/Glycine

161-0734
1 L, 10x premixed electrophoresis buffer

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10x Tris/Glycine

161-0771
5 L cube, 10x premixed electrophoresis buffer

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10x Tris/Tricine/SDS

161-0744
1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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10x IEF Anode Buffer

161-0761
250 ml, electrophoresis buffer, 7 mM phosphoric acid, for use in isoelectric focusing

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10x IEF Cathode Buffer

161-0762
250 ml, electrophoresis buffer, 20 mM lysine, 20 mM arginine, for use in isoelectric focusing

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10x Zymogram Renaturation Buffer

161-0765
125 ml, 10x premixed electrophoresis buffer, contains 2.5% Triton X-100, for use with Zymogram gels

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10x Zymogram Development Buffer

161-0766
125 ml, 10x premixed electrophoresis buffer, contains 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM CaCl2, for use with Zymogram gels

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XT MOPS Running Buffer

161-0788
500 ml, 20x electrophoresis buffer for use with Criterion XT Bis-Tris gels

List Price:   $61.00
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XT MES Running Buffer

161-0789
500 ml, 20x electrophoresis buffer for use with Criterion XT Bis-Tris gels

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XT Tricine Running Buffer

161-0790
500 ml, 20x electrophoresis buffer for use with Criterion XT Tris-Acetate gels

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XT MOPS Buffer Kit

161-0793
Electrophoresis buffer kit, includes 500 ml 20x XT MOPS running buffer, 10 ml 4x XT sample buffer, 1 ml 20x XT reducing agent

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XT MES Buffer Kit

161-0796
Electrophoresis buffer kit, includes 500 ml 20x XT MES running buffer, 10 ml 4x XT sample buffer, 1 ml 20x XT reducing agent

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XT Tricine Buffer Kit

161-0797
Electrophoresis buffer kit, includes 500 ml 20x XT Tricine running buffer, 10 ml 4x XT sample buffer, 1 ml 20x XT reducing agent

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Glycine

161-0717
250 g, glycine (aminoacetic acid) powder

List Price:   $23.00
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Glycine

161-0718
1 kg, glycine (aminoacetic acid) powder

List Price:   $50.00
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Glycine

161-0724
2 kg, glycine (aminoacetic acid) powder

List Price:   $99.00
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Tricine

161-0713
500 g, Tricine (N-Tris[hydroxymethyl]methylglycine) powder

List Price:   $189.00
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Tris

161-0716
500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

List Price:   $32.00
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Tris

161-0719
1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

List Price:   $79.00
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Urea

161-0730
250 g, urea (carbamide)

List Price:   $26.00
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Urea

161-0731
1 kg, urea (carbamide)

List Price:   $71.00
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Number Description Options
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]