Many real-time PCR thermal cyclers require an internal ROX reference dye for fluorescent signal normalization and correction of well-to-well optical variations. Bio-Rad's ROX Passive Reference Dye allows PCR reagents that do not contain ROX to be used on all ROX-dependent real-time PCR systems.
As the excitation optics of different instrument platforms vary, the optimal ROX reference dye concentration must be matched to each real-time PCR cycler. This ROX reference dye is formulated as a 50x concentrated stock solution for use on the following instruments:
- ABI PRISM 7000, 7300, 7700, and 7900 systems
This ROX reference dye should be treated as a 750x concentrated solution for systems that use 580–585 nm excitation for passive reference dye, such as the following instruments:
- Stratagene Mx4000, Mx3000P, or Mx3005P systems
- ABI PRISM 7500 sequence detection system
Note: An internal ROX reference dye is not required for use with any Bio-Rad real-time PCR detection system.