The iScript Select cDNA synthesis kit is a sensitive reverse transcription system offering flexible priming strategies. Unlike our iScript cDNA synthesis kit and iScript reverse transcription supermix for RT-qPCR, this kit comes with multiple reaction components (5x buffer, oligo[dT], random primers, proprietary gene-specific primer enhancer solution, and water) that must be combined during reaction setup.
Key Features and Benefits
- Have flexibility in mRNA priming strategy — oligo(dT), random primer, and gene-specific primer enhancer solution provided in separate tubes
- Synthesize long cDNAs up to 8 kb — quality controlled for reliable synthesis of cDNA >6 kb in length
- Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
- Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription