iScript™ Select cDNA Synthesis Kit

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Overview

Broad Dynamic Range

The iScript™ Select cDNA synthesis kit performs reliably over six orders of magnitude using any first-strand priming approach. Human total RNA from 1 pg to 1 µg was reverse transcribed using the iScript Select cDNA synthesis kit and the first-strand primers indicated above. The resulting cDNA was amplified in triplicate using iQ™ SYBR® Green supermix with β-actin primers and detected using the iCycler iQ® real-time detection system, RFU, relative fluorescence units.

Long cDNA Synthesis

The iScript Select cDNA synthesis kit facilitates synthesis of cDNA longer than 6 kb. First-strand cDNA was produced from 1 µg total RNA using the iScript Select cDNA synthesis kit and the provided oligo(dT) primer mix. A 2 µl aliquot of the 20 µl cDNA synthesis reaction was subjected to 35 PCR cycles using a proofreading high-fidelity polymerase and human adenomatous polyposis coli (APC) primer sets. From a 50 µl PCR reaction, 10 µl was analyzed on a 1% agarose gel. Lane 1, 1 kb ladder; lanes 2 and 3, 6.4 kb APC PCR product; lanes 4 and 5, 8.2 kb APC PCR product.

The iScript Select cDNA synthesis kit is a sensitive reverse transcription system offering flexible priming strategies. Unlike our iScript cDNA synthesis kit and iScript reverse transcription supermix for RT-qPCR, this kit comes with multiple reaction components (5x buffer, oligo[dT], random primers, proprietary gene-specific primer enhancer solution, and water) that must be combined during reaction setup.

Key Features and Benefits

  • Have flexibility in mRNA priming strategy — oligo(dT), random primer, and gene-specific primer enhancer solution provided in separate tubes
  • Synthesize long cDNAs up to 8 kb — quality controlled for reliable synthesis of cDNA >6 kb in length
  • Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
iScript™ Select cDNA Synthesis Kit

170-8896
25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

List Price:   $135.00
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iScript™ Select cDNA Synthesis Kit

170-8897
100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

List Price:   $430.00
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iScript™ Select cDNA Synthesis Kit

170-8897BUN
500 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

List Price:   $2,035.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
10001023 Instruction Manual, iScript Select cDNA Synthesis Kit, Rev B Click to download
2894 iScript cDNA Synthesis Kits Flier, Rev F Click to download
5438 Convenient Multiplex PCR Assays With iQ Multiplex Powermix, Rev A Click to download