iScript Select cDNA Synthesis Kit

iScript Select cDNA Synthesis Kit

A high-fidelity reverse transcription kit that also provides flexibility in primer setup (individual tubes of oligo(dT) and random primers).

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Overview

The iScript Select cDNA Synthesis Kit is a high-fidelity, sensitive reverse transcription system that provides ultimate flexibility. Unlike our other iScript cDNA Synthesis products, the reaction components for this kit are packaged in individual tubes, enabling flexible priming options and customization of reaction setup for specific application needs.

Key Features and Benefits

  • Achieve high-fidelity — unique reverse transcription kit minimizes introduction of base pair errors
  • Synthesize long cDNAs — efficiently synthesize cDNA greater than 8 kb
  • Save time and reduce errors during setup — less hands on time and less pipetting steps compared to protocols for other high-fidelity reverse transcriptase kits
  • Detect low-level target genes and conserve RNA — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription

 

High-Fidelity

high-fidelity-sign

iScript Select cDNA Synthesis Kit is designed for applications requiring high-fidelity, such as cloning and NGS. This kit introduces fewer base pair errors than other commonly used reverse transcription kits.

Ideal RT for Many Applications

The combination of iScript Select Kit performance and its packaging of various priming options in individual tubes enables this reagent to perform optimally across many different applications.

A versatile reverse transcription kit serves all of your RT-PCR needs

  • Cloning

    Cloning

  • RT-qPCR

    RT-qPCR

  • NGS

    NGS

  • Sequencing

    Sequencing

 

Selector Tools

Use our selectors to find the best reagents, plates, tubes, seals, and accessories for your PCR and qPCR experiments.

Choose the best cDNA synthesis kit for your application, with formulations optimized for ease, yield, flexibility, or gDNA clearance.

Specifications

Storage at –20°C
Guaranteed for 12 months at –20°C in a constant temperature
freezer (multiple freeze thaws not recommended)

Ordering

items

Use the filters below to refine results!

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1708896
25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
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1708897
100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
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1708897BUN
500 x 20 µl reactions, includes 5x reaction mix, iScript Reverse Transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

Documents

Number Description Options
10001023
Instruction Manual, iScript™ Select cDNA Synthesis Kit, Rev E
7075
iScript™ Select cDNA Synthesis Kit Flier
7076
iScript™ Select cDNA Synthesis Kit Has Highest Fidelity as Determined by Next-Generation Sequencing
7037
iScript High-Fidelity Cloning Applications Protocol
6746
iScript Reverse Transcription Kits Flier
[ Add to Cart (Free) ]
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
5211
iProof High-Fidelity DNA Polymerase Flier, Rev C
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
6090
Amplification Reagents and Plastics Brochure