Real-Time PCR Detection Systems for In Vitro Diagnostics (IVD)

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Real-Time PCR Detection Systems for In Vitro Diagnostics (IVD)


CFX96™ IVD Real-Time PCR Systems offer the ultimate flexibility in commercial assay selection or rapid assay development in a clinical laboratory setting. The instruments offer precise quantification and target discrimination for up to five targets, with accurate thermal cycler performance and powerful easy-to-use software.


CFX96 Real-Time PCR Detection System—IVD* CFX96 Deep Well
Real-Time PCR Detection System—IVD*
10–50 µl reaction volumes 10–125 µl reaction volumes
Up to 5-target multiplexing for reduced sample and reagent use
Quickly and accurately analyze and validate data using the intuitive CFX Manager™ IVD Software
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Resources

Consumables PCR plastics, including plates, tubes, tube strips, and seals
Documentation User Manual Data Sheet User Manual Data Sheet

Specifications

Platform CFX96™ Real-Time PCR
Detection System–IVD		 CFX96™ Deep Well Real-Time
PCR Detection System–IVD

Supported Format 96-well plate
8-well strips

Thermal Cycler

  C1000™ Thermal Cycler
Maximum ramp rate 5°C/sec 2.5°C/sec
Average ramp rate 3.3°C/sec 2.0°C/sec
Heating and cooling
method		 Peltier
Lid Heats up to 105°C

Temperature

Range 0–100°C
Accuracy ±0.2°C of programmed target at 90°C
Uniformity ±0.4°C well-to-well within 10 sec of arrival at 90°C

Gradient

Operational range 30–100°C
Programmable span 1–24°C

Optical Detection

  CFX96 Optical Reaction Module CFX96 Deep Well Optical Reaction Module
Excitation 6 filtered LEDs
Detection 6 filtered photodiodes
Range of
excitation/emission
wavelengths		 450–730 nm
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Calibrated dye FAM, SYBR® Green, HEX, VIC, Quasar 670, Quasar 705, ROX, TEX 615, Texas Red, Cy5, Cy5.5, CAL Fluor Gold 540, CAL Fluor Orange 560, CAL Fluor Red 610

Scan Time

All channels 12 sec
FAM/SYBR® Green only 3 sec

Software

Operating systems Windows 7, Windows 8
Multiplex analysis Up to 5 targets per well
Laboratory information
system (LIS) Compatible		 Yes

System

Licensed for real-time PCR Yes
Sample capacity 12 x 8-tube strips
1 x 96-well plate (fast)
Sample size 10–50 µl (10–25 µl
recommended)		 10–125 µl
Sample throughput 4 to 5 x 96-well plates per day
Communication interface USB 2.0
Electrical approvals IEC, CE
Dimensions (W x D x H) 33 x 46 x 36 cm (12 x 18 x 14 in.)
Weight 21 kg (47 lb)

*Legal Notice:

The CFX96 Real-Time PCR Detection System—IVD is intended for use in the fields of research and human in vitro diagnostics. The system is CE-IVD marked in compliance with the European Union diagnostic medical device manufacturing standards.

Europe: For in vitro diagnostic use. The CFX96 Real-Time PCR Detection System—IVD and CFX96 Deep Well Real-Time PCR Detection System—IVD meet the requirements of the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–registered CFX96 Real-Time PCR Detection System—IVD and CFX96 Deep Well IVD Real-Time Detection PCR System—IVD Instruments are for distribution and use in specific European countries only (Austria, Belgium, Switzerland, Germany, Denmark, Spain, Finland, France, United Kingdom, Greece, Ireland, Italy, Liechtenstein, Luxembourg, Monaco, Netherlands, Portugal, Sweden).

USA: The CFX96 Real-Time Detection System—IVD and CFX96 Deep Well Real-Time Detection System—IVD are not available for sale in the U.S.A.

No rights are granted by Bio-Rad for the use of melt curve analysis in the field of human in vitro diagnostics. In addition, it is the responsiblity of the purchaser to obtain any intellectual property rights which may be required for its specific applications.