ReadyPrep™ Reagents for Extraction and Solubilization

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ReadyPrep Reagents for Extraction and Solubilization

Overview

ReadyPrep Buffers
Product Name Description Volume Catalog #
ReadyPrep 2-D rehydration/sample buffer 1 7 M urea, 2 M thiourea, 1% (w/v) ASB-14 detergent, 40 mM Tris base, 0.001% Bromophenol Blue 10 ml 163–2083
ReadyPrep 2-D starter kit rehydration/sample buffer 8 M urea, 2% CHAPS, 50 mM DTT, 0.2% Bio-Lyte 3/10 ampholyte, 0.001% Bromophenol Blue 10 ml 163–2106
ReadyPrep sequential extraction kit reagent 1 40 mM Tris base 50 ml 163–2102
ReadyPrep sequential extraction kit reagent 2 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte 10 ml 163–2103
ReadyPrep sequential extraction kit reagent 3 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3–10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte 10 ml 163–2104
Protein Solubilization buffer (PSB) Urea, thiourea, CHAPS, NDSB 201 50 ml 163–2145
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A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation). Some of the premixed buffer and reagent components of these kits are also available separately for use in customized protein extraction and solubilization protocols.

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ReadyPrep™ 2-D Rehydration/Sample Buffer 1

1632083
10 ml, protein solubilization reagent, includes 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 0.001% Bromophenol Blue

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ReadyPrep™ 2-D Starter Kit Rehydration/Sample Buffer

1632106
10 ml, protein sample buffer, includes 8 M urea, 2% CHAPS, 50 mM DTT, 0.2% Bio-Lyte® 3/10 ampholyte, 0.001% Bromophenol Blue

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ReadyPrep™ Sequential Extraction Kit Reagent 1

1632102
50 ml, 40 mM Tris base, for protein preparation and fractionation buffers; component of kit #163-2100

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ReadyPrep™ Sequential Extraction Kit Reagent 2

1632103
10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100

3 1632103 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2103_0921120738361.xml 1632103 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:59 1632103-readyprep-sequential-extraction-kit-reagent-2 1632103 ReadyPrep Sequential Extraction Kit Reagent 2 ReadyPrep&#153; Sequential Extraction Kit Reagent 2 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2103_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2103_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2103_feature.jpg preview RepEx 163-2103 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Protein-Sample-Preparation/Protein-Fractionation N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632103&CartQuote=QT&quantity= 1432 false true false $39.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632103&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632103&quantity= 1632103
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ReadyPrep™ Sequential Extraction Kit Reagent 3

1632104
10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100

4 1632104 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2104_0921120738380.xml 1632104 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:59 1632104-readyprep-sequential-extraction-kit-reagent-3 1632104 ReadyPrep Sequential Extraction Kit Reagent 3 ReadyPrep&#153; Sequential Extraction Kit Reagent 3 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2104_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2104_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2104_feature.jpg preview RepEx 163-2104 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Protein-Sample-Preparation/Protein-Fractionation N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632104&CartQuote=QT&quantity= 1432 true true false $39.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632104&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632104&quantity= 1632104
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Protein Solubilization Buffer (PSB)

1632145
Pkg of 1, strongly chaotropic protein solubilization buffer, contains NDSB 201, urea, thiourea, and CHAPS, makes 50 ml of solution

5 1632145 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Extraction-/163-2145_0921120743035.xml 1632145 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcrucyyajcrucyyb" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcrucyyajcrucyyb"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="25" DayOfWeek="4" Year="2018" Hour="0" Minute="38" Second="51" ZoneOffsetMillis="0" Time="1516840731851">Thu Jan 25 00:38:51 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Extraction-/163-2145_0921120743035.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="25" DayOfWeek="4" Year="2018" Hour="0" Minute="38" Second="51" ZoneOffsetMillis="0" Time="1516840731851">Thu Jan 25 00:38:51 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:07 PM en_US jcrucy95 2017-11-14 07:08:59 true true internet/product_SKU LSR N en 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:59 1632145-protein-solubilization-buffer-psb 1632145 Protein Solubilization Buffer (PSB) Protein Solubilization Buffer (PSB) Pkg of 1, strongly chaotropic protein solubilization buffer, contains NDSB 201, urea, thiourea, and CHAPS, makes 50 ml of solution Pkg of 1, strongly chaotropic protein solubilization buffer, contains NDSB 201, urea, thiourea, and CHAPS, makes 50 ml of solution Pkg of 1, strongly chaotropic protein solubilization buffer, contains NDSB 201, urea, thiourea, and CHAPS, makes 50 ml of solution /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2145_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2145_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2145_feature.jpg RepEx 163-2145 Pkg of 1, strongly chaotropic protein solubilization buffer, contains NDSB 201, urea, thiourea, and CHAPS, makes 50 ml of solution 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Protein-Sample-Preparation/Protein-Extraction- N 0 1632145, protein, solubilization, buffer, psb, strong, chaotropic, ndsb, 201, urea, thiourea, chaps, extraction, fractionation false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632145&CartQuote=QT&quantity= 1432 true true false $138.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632145&AddToDefaultWishList=true&quantity= true false true 12161700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632145&quantity= 1632145
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ReadyPrep™ Proteomics Grade Water

1632091
500 ml, ultrapure water for use with ReadyPrep fractionation and general purpose cleanup kits

6 1632091 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Experion-Automated-Electrophor/163-2091_0921120319815.xml 1632091 09/21/09 12:03 PM N en 09/21/19 12:03 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:10 1632091-readyprep-proteomics-grade-water 1632091 ReadyPrep Proteomics Grade Water ReadyPrep™ Proteomics Grade Water 500 ml, ultrapure water for use with ReadyPrep fractionation and general purpose cleanup kits 500 ml, ultrapure water for use with ReadyPrep fractionation and general purpose cleanup kits 500 ml, ultrapure water for use with ReadyPrep fractionation and general purpose cleanup kits /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2091_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2091_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2091_feature.jpg preview RepEx 163-2091 500 ml, ultrapure water for use with ReadyPrep fractionation and general purpose cleanup kits 1632091, readyprep, proteomics, grade, water, ultrapure, fractionation, general, purpose, cleanup 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Experion-Automated-Electrophor N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632091&CartQuote=QT&quantity= 1432 false true false $43.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632091&AddToDefaultWishList=true&quantity= true false true 41116100 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632091&quantity= 1632091
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4006167 Instruction Manual, ReadyPrep™ Sequential Extraction Kit, Rev B Click to download

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