The ReadyPrep protein extraction kit (total protein) provides a simple, rapid, and reproducible method for preparation of total cellular protein extracts from a wide variety of biological samples. Use of this kit generates protein samples that can be applied directly to a variety of applications, including IEF and 2-D gel separation.
The ReadyPrep protein extraction kit (total protein):
- Includes a strongly chaotropic extraction solution containing the zwitterionic detergent ASB-14
- Provides reagents for up to 20 extractions, each using 50–100 mg of cells or tissue
- Can easily be scaled up to accommodate larger amounts of cells or tissue
- Generates total protein samples in about 45 minutes