ReadyPrep™ Protein Extraction Kit (Total Protein)

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ReadyPrep Protein Extraction Kit (Total Protein)




The ReadyPrep protein extraction kit (total protein) provides a simple, rapid, and reproducible method for preparation of total cellular protein extracts from a wide variety of biological samples. Use of this kit generates protein samples that can be applied directly to a variety of applications, including IEF and 2-D gel separation.

The ReadyPrep protein extraction kit (total protein):

  • Includes a strongly chaotropic extraction solution containing the zwitterionic detergent ASB-14
  • Provides reagents for up to 20 extractions, each using 50–100 mg of cells or tissue
  • Can easily be scaled up to accommodate larger amounts of cells or tissue
  • Generates total protein samples in about 45 minutes

 

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ReadyPrep™ Protein Extraction Kit (Total Protein)

1632086
20 preps, general purpose protein preparation kit, includes strong detergent ASB-14

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Tributylphosphine (TBP)

1632101
0.6 ml, 200 mM, reducing agent for use in protein sample preparation applications

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ReadyPrep™ 2-D Rehydration/Sample Buffer 1

1632083
10 ml, protein solubilization reagent, includes 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 0.001% Bromophenol Blue

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Number Description Options
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download
4110159 Instruction Manual, ReadyPrep Protein Extraction Kit (Total Protein), Rev A Click to download

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