ReadyPrep™ Protein Extraction Kit (Membrane II)



Fractionation of Proteins by Cellular Location

Differences in 2-D patterns using ReadyPrep signal (A), membrane I (B), and membrane II (C) kits. Mouse liver samples were extracted using the recommended protocols for each kit. Purified protein (~450 µg) was focused on 17 cm pH 3–10 nonlinear ReadyStrip IPG strips and run on 8–16% PROTEAN II precast gels for the second dimension. Overall spot patterns differ for A, B, and C even though all three kits isolate membrane proteins, indicating each kit isolates different types of membrane proteins.

The ReadyPrep protein extraction kit (membrane II) applies sodium carbonate extraction methods (Fujiki et al. 1982, Molloy et al. 2000) to fractionate and isolate membrane proteins.

The ReadyPrep protein extraction kit (membrane II):

  • Isolates complex proteins that contain more than two transmembrane domains or those that are resistant to extraction by the ReadyPrep protein extraction (membrane I) kit
  • Requires ultracentrifugation
  • Includes a strongly chaotropic, IEF-compatible rehydration/sample buffer that contains the detergent ASB-14 to efficiently solubilize proteins


Fujiki Y et al. (1982). Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biology 93, 97–102.
Molloy MP et al. (2000). Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem 267, 2871–2881.


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ReadyPrep™ Protein Extraction Kit (Membrane II), 10 preps
ReadyPrep™ Protein Extraction Kit (Membrane II), 10 preps

10 preps, includes 2 x 5 ml 2-D Rehydration/Sample Buffer 1, 50 ml lysis buffer, 125 ml membrane protein concentrating reagent, 0.6 ml 200 mM tributylphosphine (TBP), 1 storage vial, instruction manual; for 10 extractions of 100–200 mg of cells or tissue

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4110157 Instruction Manual, ReadyPrep™ Protein Extraction Kit (Membrane II), Rev A Click to download